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Q2NKI2

- VAX1_MOUSE

UniProt

Q2NKI2 - VAX1_MOUSE

Protein

Ventral anterior homeobox 1

Gene

Vax1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 2 (27 Jun 2006)
      Previous versions | rss
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    Functioni

    Transcription factor that may function in dorsoventral specification of the forebrain. Required for axon guidance and major tract formation in the developing forebrain. May contribute to the differentiation of the neuroretina, pigmented epithelium and optic stalk.4 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi100 – 15960HomeoboxPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. chromatin DNA binding Source: MGI
    2. sequence-specific DNA binding transcription factor activity Source: MGI
    3. transcription regulatory region sequence-specific DNA binding Source: InterPro

    GO - Biological processi

    1. axon guidance Source: MGI
    2. brain development Source: MGI
    3. camera-type eye development Source: MGI
    4. cell differentiation Source: MGI
    5. central nervous system development Source: MGI
    6. negative regulation of neuroblast proliferation Source: MGI
    7. negative regulation of transcription from RNA polymerase II promoter Source: MGI
    8. nervous system development Source: MGI
    9. neuron migration Source: MGI
    10. palate development Source: MGI
    11. skeletal muscle cell differentiation Source: MGI
    12. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ventral anterior homeobox 1
    Gene namesi
    Name:Vax1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 19

    Organism-specific databases

    MGIiMGI:1277163. Vax1.

    Subcellular locationi

    Nucleus PROSITE-ProRule annotation

    GO - Cellular componenti

    1. nucleus Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 338338Ventral anterior homeobox 1PRO_0000240523Add
    BLAST

    Proteomic databases

    PRIDEiQ2NKI2.

    PTM databases

    PhosphoSiteiQ2NKI2.

    Expressioni

    Developmental stagei

    At 9.5 dpc, expressed in the forebrain, through the optic stalk and ending in the proximal portion of the optic vesicle. At 10.5 dpc, expressed in the optic stalk, outer layer of the optic cup, and the primordium of the pigment epithelium. In the pigment epithelium, down-regulated after 11.5 dpc, and expression restricted to the optic stalk and the ventral forebrain.2 Publications

    Gene expression databases

    BgeeiQ2NKI2.
    CleanExiMM_VAX1.
    GenevestigatoriQ2NKI2.

    Structurei

    3D structure databases

    ProteinModelPortaliQ2NKI2.
    SMRiQ2NKI2. Positions 102-157.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi216 – 27257Ala-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the EMX homeobox family.Curated
    Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Homeobox

    Phylogenomic databases

    eggNOGiNOG323848.
    GeneTreeiENSGT00750000117644.
    HOGENOMiHOG000049217.
    HOVERGENiHBG007558.
    InParanoidiQ2NKI2.
    KOiK09318.
    OMAiASEDCNK.
    PhylomeDBiQ2NKI2.
    TreeFamiTF319504.

    Family and domain databases

    Gene3Di1.10.10.60. 1 hit.
    InterProiIPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR000047. HTH_motif.
    [Graphical view]
    PfamiPF00046. Homeobox. 1 hit.
    [Graphical view]
    PRINTSiPR00031. HTHREPRESSR.
    SMARTiSM00389. HOX. 1 hit.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 1 hit.
    PROSITEiPS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q2NKI2-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MFGKPDKMDV RCHSDTEAAR VSKNAHKESR EIKGAEGSLP AAFLKEPQGA    50
    FSGSGASEDC NKSKSNSSAD PDYCRRILVR DAKGSIREII LPKGLDLDRP 100
    KRTRTSFTAE QLYRLEMEFQ RCQYVVGRER TELARQLNLS ETQVKVWFQN 150
    RRTKQKKDQG KDSELRSVVS ETAATCSVLR LLEQGRLLSP PGLPALLPPC 200
    ATGALGSALR GPSLPALGAG AAAGSAAAAA AAAAATAPGP AGAASQHQPA 250
    VGGAPGPGPA GPGGLHAGAP TASHGLFSLP VPSLLGSVAS RLSSAPLTMA 300
    GSLAGNLQEL SARYLSSSAF EPYSRTNNKE GAEKKALD 338
    Length:338
    Mass (Da):35,167
    Last modified:June 27, 2006 - v2
    Checksum:i072986819244BB81
    GO
    Isoform 2 (identifier: Q2NKI2-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         212-338: PSLPALGAGA...KEGAEKKALD → RTARGSTDCQ...GLRALLPDQQ

    Note: No experimental confirmation available.

    Show »
    Length:276
    Mass (Da):30,487
    Checksum:i150DB6713DD86CE7
    GO

    Sequence cautioni

    The sequence AAI11819.1 differs from that shown. Reason: Erroneous initiation.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei212 – 338127PSLPA…KKALD → RTARGSTDCQPRSLQPAGAV AARLCRQPPVLRPVDDGWFA SREFARTLSPLPELLGLRAL LPDQQ in isoform 2. 1 PublicationVSP_019397Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF064554 mRNA. Translation: AAC36319.1.
    AB020495 Genomic DNA. Translation: BAA76866.1.
    BC111818 mRNA. Translation: AAI11819.1. Different initiation.
    CCDSiCCDS29933.1. [Q2NKI2-1]
    RefSeqiNP_033527.1. NM_009501.1. [Q2NKI2-1]
    UniGeneiMm.23801.

    Genome annotation databases

    EnsembliENSMUST00000172821; ENSMUSP00000134642; ENSMUSG00000006270. [Q2NKI2-1]
    GeneIDi22326.
    KEGGimmu:22326.
    UCSCiuc008ibg.1. mouse. [Q2NKI2-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF064554 mRNA. Translation: AAC36319.1 .
    AB020495 Genomic DNA. Translation: BAA76866.1 .
    BC111818 mRNA. Translation: AAI11819.1 . Different initiation.
    CCDSi CCDS29933.1. [Q2NKI2-1 ]
    RefSeqi NP_033527.1. NM_009501.1. [Q2NKI2-1 ]
    UniGenei Mm.23801.

    3D structure databases

    ProteinModelPortali Q2NKI2.
    SMRi Q2NKI2. Positions 102-157.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q2NKI2.

    Proteomic databases

    PRIDEi Q2NKI2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000172821 ; ENSMUSP00000134642 ; ENSMUSG00000006270 . [Q2NKI2-1 ]
    GeneIDi 22326.
    KEGGi mmu:22326.
    UCSCi uc008ibg.1. mouse. [Q2NKI2-1 ]

    Organism-specific databases

    CTDi 11023.
    MGIi MGI:1277163. Vax1.

    Phylogenomic databases

    eggNOGi NOG323848.
    GeneTreei ENSGT00750000117644.
    HOGENOMi HOG000049217.
    HOVERGENi HBG007558.
    InParanoidi Q2NKI2.
    KOi K09318.
    OMAi ASEDCNK.
    PhylomeDBi Q2NKI2.
    TreeFami TF319504.

    Miscellaneous databases

    NextBioi 302557.
    PROi Q2NKI2.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q2NKI2.
    CleanExi MM_VAX1.
    Genevestigatori Q2NKI2.

    Family and domain databases

    Gene3Di 1.10.10.60. 1 hit.
    InterProi IPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR000047. HTH_motif.
    [Graphical view ]
    Pfami PF00046. Homeobox. 1 hit.
    [Graphical view ]
    PRINTSi PR00031. HTHREPRESSR.
    SMARTi SM00389. HOX. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 1 hit.
    PROSITEi PS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Vax1 is a novel homeobox-containing gene expressed in the developing anterior ventral forebrain."
      Hallonet M., Hollemann T., Wehr R., Jenkins N.A., Copeland N.G., Pieler T., Gruss P.
      Development 125:2599-2610(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, DEVELOPMENTAL STAGE.
      Tissue: Brain.
    2. "Expression of the Vax family homeobox genes suggests multiple roles in eye development."
      Ohsaki K., Morimitsu T., Ishida Y., Kominami R., Takahashi N.
      Genes Cells 4:267-276(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1), FUNCTION, DEVELOPMENTAL STAGE.
      Strain: BALB/c.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-338 (ISOFORM 2).
    4. "The homeodomain protein vax1 is required for axon guidance and major tract formation in the developing forebrain."
      Bertuzzi S., Hindges R., Mui S.H., O'Leary D.D., Lemke G.
      Genes Dev. 13:3092-3105(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "A novel mechanism for the transcriptional regulation of Wnt signaling in development."
      Vacik T., Stubbs J.L., Lemke G.
      Genes Dev. 25:1783-1795(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiVAX1_MOUSE
    AccessioniPrimary (citable) accession number: Q2NKI2
    Secondary accession number(s): O88880
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 27, 2006
    Last sequence update: June 27, 2006
    Last modified: October 1, 2014
    This is version 79 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3