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Q2NKI2 (VAX1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ventral anterior homeobox 1
Gene names
Name:Vax1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length338 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Transcription factor that may function in dorsoventral specification of the forebrain. Required for axon guidance and major tract formation in the developing forebrain. May contribute to the differentiation of the neuroretina, pigmented epithelium and optic stalk. Ref.1 Ref.2 Ref.4 Ref.5

Subcellular location

Nucleus By similarity.

Developmental stage

At 9.5 dpc, expressed in the forebrain, through the optic stalk and ending in the proximal portion of the optic vesicle. At 10.5 dpc, expressed in the optic stalk, outer layer of the optic cup, and the primordium of the pigment epithelium. In the pigment epithelium, down-regulated after 11.5 dpc, and expression restricted to the optic stalk and the ventral forebrain. Ref.1 Ref.2

Sequence similarities

Belongs to the EMX homeobox family.

Contains 1 homeobox DNA-binding domain.

Sequence caution

The sequence AAI11819.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   DomainHomeobox
   LigandDNA-binding
   Molecular functionDevelopmental protein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processaxon guidance

Inferred from mutant phenotype Ref.4. Source: MGI

brain development

Inferred from mutant phenotype Ref.4PubMed 10601036. Source: MGI

camera-type eye development

Inferred from genetic interaction PubMed 15905411. Source: MGI

cell differentiation

Inferred from mutant phenotype PubMed 15590934. Source: MGI

central nervous system development

Inferred from mutant phenotype Ref.4PubMed 10601036. Source: MGI

negative regulation of neuroblast proliferation

Inferred from mutant phenotype PubMed 15590934. Source: MGI

negative regulation of transcription from RNA polymerase II promoter

Inferred from genetic interaction PubMed 15905411. Source: MGI

nervous system development

Inferred from mutant phenotype PubMed 15280216. Source: MGI

neuron migration

Inferred from mutant phenotype PubMed 15280216PubMed 15590934. Source: MGI

palate development

Inferred from mutant phenotype Ref.4. Source: MGI

skeletal muscle cell differentiation

Inferred from mutant phenotype PubMed 22147266. Source: MGI

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from direct assay PubMed 17043310. Source: MGI

   Molecular_functionchromatin DNA binding

Inferred from direct assay PubMed 15905411. Source: MGI

sequence-specific DNA binding transcription factor activity

Inferred from mutant phenotype Ref.4. Source: MGI

transcription regulatory region sequence-specific DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q2NKI2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q2NKI2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     212-338: PSLPALGAGA...KEGAEKKALD → RTARGSTDCQ...GLRALLPDQQ
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 338338Ventral anterior homeobox 1
PRO_0000240523

Regions

DNA binding100 – 15960Homeobox
Compositional bias216 – 27257Ala-rich

Natural variations

Alternative sequence212 – 338127PSLPA…KKALD → RTARGSTDCQPRSLQPAGAV AARLCRQPPVLRPVDDGWFA SREFARTLSPLPELLGLRAL LPDQQ in isoform 2.
VSP_019397

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 27, 2006. Version 2.
Checksum: 072986819244BB81

FASTA33835,167
        10         20         30         40         50         60 
MFGKPDKMDV RCHSDTEAAR VSKNAHKESR EIKGAEGSLP AAFLKEPQGA FSGSGASEDC 

        70         80         90        100        110        120 
NKSKSNSSAD PDYCRRILVR DAKGSIREII LPKGLDLDRP KRTRTSFTAE QLYRLEMEFQ 

       130        140        150        160        170        180 
RCQYVVGRER TELARQLNLS ETQVKVWFQN RRTKQKKDQG KDSELRSVVS ETAATCSVLR 

       190        200        210        220        230        240 
LLEQGRLLSP PGLPALLPPC ATGALGSALR GPSLPALGAG AAAGSAAAAA AAAAATAPGP 

       250        260        270        280        290        300 
AGAASQHQPA VGGAPGPGPA GPGGLHAGAP TASHGLFSLP VPSLLGSVAS RLSSAPLTMA 

       310        320        330 
GSLAGNLQEL SARYLSSSAF EPYSRTNNKE GAEKKALD 

« Hide

Isoform 2 [UniParc].

Checksum: 150DB6713DD86CE7
Show »

FASTA27630,487

References

« Hide 'large scale' references
[1]"Vax1 is a novel homeobox-containing gene expressed in the developing anterior ventral forebrain."
Hallonet M., Hollemann T., Wehr R., Jenkins N.A., Copeland N.G., Pieler T., Gruss P.
Development 125:2599-2610(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, DEVELOPMENTAL STAGE.
Tissue: Brain.
[2]"Expression of the Vax family homeobox genes suggests multiple roles in eye development."
Ohsaki K., Morimitsu T., Ishida Y., Kominami R., Takahashi N.
Genes Cells 4:267-276(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1), FUNCTION, DEVELOPMENTAL STAGE.
Strain: BALB/c.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-338 (ISOFORM 2).
[4]"The homeodomain protein vax1 is required for axon guidance and major tract formation in the developing forebrain."
Bertuzzi S., Hindges R., Mui S.H., O'Leary D.D., Lemke G.
Genes Dev. 13:3092-3105(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"A novel mechanism for the transcriptional regulation of Wnt signaling in development."
Vacik T., Stubbs J.L., Lemke G.
Genes Dev. 25:1783-1795(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF064554 mRNA. Translation: AAC36319.1.
AB020495 Genomic DNA. Translation: BAA76866.1.
BC111818 mRNA. Translation: AAI11819.1. Different initiation.
CCDSCCDS29933.1. [Q2NKI2-1]
RefSeqNP_033527.1. NM_009501.1. [Q2NKI2-1]
UniGeneMm.23801.

3D structure databases

ProteinModelPortalQ2NKI2.
SMRQ2NKI2. Positions 102-157.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ2NKI2.

Proteomic databases

PRIDEQ2NKI2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000172821; ENSMUSP00000134642; ENSMUSG00000006270. [Q2NKI2-1]
GeneID22326.
KEGGmmu:22326.
UCSCuc008ibg.1. mouse. [Q2NKI2-1]

Organism-specific databases

CTD11023.
MGIMGI:1277163. Vax1.

Phylogenomic databases

eggNOGNOG323848.
GeneTreeENSGT00750000117644.
HOGENOMHOG000049217.
HOVERGENHBG007558.
InParanoidQ2NKI2.
KOK09318.
OMAASEDCNK.
PhylomeDBQ2NKI2.
TreeFamTF319504.

Gene expression databases

BgeeQ2NKI2.
CleanExMM_VAX1.
GenevestigatorQ2NKI2.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR000047. HTH_motif.
[Graphical view]
PfamPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSPR00031. HTHREPRESSR.
SMARTSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio302557.
PROQ2NKI2.
SOURCESearch...

Entry information

Entry nameVAX1_MOUSE
AccessionPrimary (citable) accession number: Q2NKI2
Secondary accession number(s): O88880
Entry history
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: June 27, 2006
Last modified: July 9, 2014
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot