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Q2NK31

- POTA_AYWBP

UniProt

Q2NK31 - POTA_AYWBP

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Protein

Spermidine/putrescine import ATP-binding protein PotA

Gene
potA, AYWB_095
Organism
Aster yellows witches'-broom phytoplasma (strain AYWB)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Part of the ABC transporter complex PotABCD involved in spermidine/putrescine import. Responsible for energy coupling to the transport system By similarity.

Catalytic activityi

ATP + H2O + polyamine(Out) = ADP + phosphate + polyamine(In).

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi37 – 448ATP Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. putrescine-importing ATPase activity Source: InterPro
  3. spermidine-importing ATPase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCPHY322098:GJ7E-98-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Spermidine/putrescine import ATP-binding protein PotA (EC:3.6.3.31)
Gene namesi
Name:potA
Ordered Locus Names:AYWB_095
OrganismiAster yellows witches'-broom phytoplasma (strain AYWB)
Taxonomic identifieri322098 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesAcholeplasmatalesAcholeplasmataceaeCandidatus PhytoplasmaCandidatus Phytoplasma asteris
ProteomesiUP000001934: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. ATP-binding cassette (ABC) transporter complex Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 417417Spermidine/putrescine import ATP-binding protein PotAPRO_0000286190Add
BLAST

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (PotA), two transmembrane proteins (PotB and PotC) and a solute-binding protein (PotD) Reviewed prediction.

Protein-protein interaction databases

STRINGi322098.AYWB_095.

Structurei

3D structure databases

ProteinModelPortaliQ2NK31.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 308304ABC transporterAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni105 – 17773InsertAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3842.
KOiK11072.
OMAiTDHVELN.
OrthoDBiEOG6T7N3V.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR017879. ABC_Sperm/Put_ATP-bd_PotA.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR008995. Mo/tungstate-bd_C_term_dom.
IPR027417. P-loop_NTPase.
IPR013611. Transp-assoc_OB_typ2.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
PF08402. TOBE_2. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF50331. SSF50331. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51305. POTA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2NK31-1 [UniParc]FASTAAdd to Basket

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MKTLIILKDL TKVFDNQLIL RGINLEIKQN EFVTLLGPSG CGKTTILRIL    50
GGFENPSSGE VLFQGKSILN VAAHKRPINT VFQKYALFPH LNVFENVAFG 100
LRLKDFNSKI KDNLDINETH YKTSKANLSK TFHQELTKDL AQEKTTQITN 150
NFNNQIEALT KDFEAKQTAL KCKKINKKEQ EEQIQKEVLK YIKIMGLQGL 200
EKRTIEQLSG GQQQRVAIAR ALINKPQVLL LDEPLSNLDL KLKQEMQYEL 250
KEIQKNSGIT FLFVTHDQEE AFTMSDKVVV MNNGEIQQIG SPEDIYNEPA 300
NRFVAQFVGE SNLIKGVMKD DFLVHFDNQT FNCVDKGFRK EENVDIVIRP 350
EDIDIVYQGK GLITGIVQSI IFKGVHWEID VKTAQRTYII HTTDHVELNK 400
KIDITFNPED IHVMEIW 417
Length:417
Mass (Da):47,776
Last modified:February 7, 2006 - v1
Checksum:i6EBD58D36DE4C5D8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000061 Genomic DNA. Translation: ABC65212.1.
RefSeqiWP_011412379.1. NC_007716.1.
YP_456291.1. NC_007716.1.

Genome annotation databases

EnsemblBacteriaiABC65212; ABC65212; AYWB_095.
GeneIDi3866049.
KEGGiayw:AYWB_095.
PATRICi21004106. VBIAstYel136969_0114.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000061 Genomic DNA. Translation: ABC65212.1 .
RefSeqi WP_011412379.1. NC_007716.1.
YP_456291.1. NC_007716.1.

3D structure databases

ProteinModelPortali Q2NK31.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 322098.AYWB_095.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABC65212 ; ABC65212 ; AYWB_095 .
GeneIDi 3866049.
KEGGi ayw:AYWB_095.
PATRICi 21004106. VBIAstYel136969_0114.

Phylogenomic databases

eggNOGi COG3842.
KOi K11072.
OMAi TDHVELN.
OrthoDBi EOG6T7N3V.

Enzyme and pathway databases

BioCyci CPHY322098:GJ7E-98-MONOMER.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR003593. AAA+_ATPase.
IPR017879. ABC_Sperm/Put_ATP-bd_PotA.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR008995. Mo/tungstate-bd_C_term_dom.
IPR027417. P-loop_NTPase.
IPR013611. Transp-assoc_OB_typ2.
[Graphical view ]
Pfami PF00005. ABC_tran. 1 hit.
PF08402. TOBE_2. 1 hit.
[Graphical view ]
SMARTi SM00382. AAA. 1 hit.
[Graphical view ]
SUPFAMi SSF50331. SSF50331. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEi PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51305. POTA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Living with genome instability: the adaptation of phytoplasmas to diverse environments of their insect and plant hosts."
    Bai X., Zhang J., Ewing A., Miller S.A., Jancso Radek A., Shevchenko D.V., Tsukerman K., Walunas T., Lapidus A., Campbell J.W., Hogenhout S.A.
    J. Bacteriol. 188:3682-3696(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AYWB.

Entry informationi

Entry nameiPOTA_AYWBP
AccessioniPrimary (citable) accession number: Q2NK31
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: February 7, 2006
Last modified: September 3, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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