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Protein

Spermidine/putrescine import ATP-binding protein PotA

Gene

potA

Organism
Aster yellows witches'-broom phytoplasma (strain AYWB)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Part of the ABC transporter complex PotABCD involved in spermidine/putrescine import. Responsible for energy coupling to the transport system.UniRule annotation

Catalytic activityi

ATP + H2O + polyamine(Out) = ADP + phosphate + polyamine(In).UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi37 – 448ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. putrescine-importing ATPase activity Source: InterPro
  3. spermidine-importing ATPase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCPHY322098:GJ7E-98-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Spermidine/putrescine import ATP-binding protein PotAUniRule annotation (EC:3.6.3.31UniRule annotation)
Gene namesi
Name:potAUniRule annotation
Ordered Locus Names:AYWB_095
OrganismiAster yellows witches'-broom phytoplasma (strain AYWB)
Taxonomic identifieri322098 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesAcholeplasmatalesAcholeplasmataceaeCandidatus PhytoplasmaCandidatus Phytoplasma asteris
ProteomesiUP000001934: Chromosome

Subcellular locationi

Cell membrane UniRule annotation; Peripheral membrane protein UniRule annotation

GO - Cellular componenti

  1. ATP-binding cassette (ABC) transporter complex Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 417417Spermidine/putrescine import ATP-binding protein PotAPRO_0000286190Add
BLAST

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (PotA), two transmembrane proteins (PotB and PotC) and a solute-binding protein (PotD).UniRule annotation

Protein-protein interaction databases

STRINGi322098.AYWB_095.

Structurei

3D structure databases

ProteinModelPortaliQ2NK31.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 308304ABC transporterUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni105 – 17773InsertAdd
BLAST

Sequence similaritiesi

Belongs to the ABC transporter superfamily. Spermidine/putrescine importer (TC 3.A.1.11.1) family. [View classification]UniRule annotation
Contains 1 ABC transporter domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG3842.
KOiK11072.
OMAiTDHVELN.
OrthoDBiEOG6T7N3V.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR017879. ABC_Sperm/Put_ATP-bd_PotA.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR008995. Mo/tungstate-bd_C_term_dom.
IPR027417. P-loop_NTPase.
IPR013611. Transp-assoc_OB_typ2.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
PF08402. TOBE_2. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF50331. SSF50331. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51305. POTA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2NK31-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKTLIILKDL TKVFDNQLIL RGINLEIKQN EFVTLLGPSG CGKTTILRIL
60 70 80 90 100
GGFENPSSGE VLFQGKSILN VAAHKRPINT VFQKYALFPH LNVFENVAFG
110 120 130 140 150
LRLKDFNSKI KDNLDINETH YKTSKANLSK TFHQELTKDL AQEKTTQITN
160 170 180 190 200
NFNNQIEALT KDFEAKQTAL KCKKINKKEQ EEQIQKEVLK YIKIMGLQGL
210 220 230 240 250
EKRTIEQLSG GQQQRVAIAR ALINKPQVLL LDEPLSNLDL KLKQEMQYEL
260 270 280 290 300
KEIQKNSGIT FLFVTHDQEE AFTMSDKVVV MNNGEIQQIG SPEDIYNEPA
310 320 330 340 350
NRFVAQFVGE SNLIKGVMKD DFLVHFDNQT FNCVDKGFRK EENVDIVIRP
360 370 380 390 400
EDIDIVYQGK GLITGIVQSI IFKGVHWEID VKTAQRTYII HTTDHVELNK
410
KIDITFNPED IHVMEIW
Length:417
Mass (Da):47,776
Last modified:February 7, 2006 - v1
Checksum:i6EBD58D36DE4C5D8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000061 Genomic DNA. Translation: ABC65212.1.
RefSeqiYP_456291.1. NC_007716.1.

Genome annotation databases

EnsemblBacteriaiABC65212; ABC65212; AYWB_095.
GeneIDi3866049.
KEGGiayw:AYWB_095.
PATRICi21004106. VBIAstYel136969_0114.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000061 Genomic DNA. Translation: ABC65212.1.
RefSeqiYP_456291.1. NC_007716.1.

3D structure databases

ProteinModelPortaliQ2NK31.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi322098.AYWB_095.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABC65212; ABC65212; AYWB_095.
GeneIDi3866049.
KEGGiayw:AYWB_095.
PATRICi21004106. VBIAstYel136969_0114.

Phylogenomic databases

eggNOGiCOG3842.
KOiK11072.
OMAiTDHVELN.
OrthoDBiEOG6T7N3V.

Enzyme and pathway databases

BioCyciCPHY322098:GJ7E-98-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR017879. ABC_Sperm/Put_ATP-bd_PotA.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR008995. Mo/tungstate-bd_C_term_dom.
IPR027417. P-loop_NTPase.
IPR013611. Transp-assoc_OB_typ2.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
PF08402. TOBE_2. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF50331. SSF50331. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51305. POTA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Living with genome instability: the adaptation of phytoplasmas to diverse environments of their insect and plant hosts."
    Bai X., Zhang J., Ewing A., Miller S.A., Jancso Radek A., Shevchenko D.V., Tsukerman K., Walunas T., Lapidus A., Campbell J.W., Hogenhout S.A.
    J. Bacteriol. 188:3682-3696(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AYWB.

Entry informationi

Entry nameiPOTA_AYWBP
AccessioniPrimary (citable) accession number: Q2NK31
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: February 7, 2006
Last modified: January 7, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.