Q2NK31 (POTA_AYWBP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Spermidine/putrescine import ATP-binding protein PotA
EC=3.6.3.31

Gene names

Name:	potA
Ordered Locus Names:	AYWB_095

Organism

Aster yellows witches'-broom phytoplasma (strain AYWB) [Complete proteome] [HAMAP]

Taxonomic identifier

322098 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Acholeplasmatales › Acholeplasmataceae › Candidatus Phytoplasma › Candidatus Phytoplasma asteris › Aster yellows witches'-broom phytoplasma

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Part of the ABC transporter complex PotABCD involved in spermidine/putrescine import. Responsible for energy coupling to the transport system By similarity.
Catalytic activity	ATP + H2O + polyamine(Out) = ADP + phosphate + polyamine(In).
Subunit structure	The complex is composed of two ATP-binding proteins (PotA), two transmembrane proteins (PotB and PotC) and a solute-binding protein (PotD) Potential.
Subcellular location	Cell membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the ABC transporter superfamily. Spermidine/putrescine importer (TC 3.A.1.11.1) family. [View classification] Contains 1 ABC transporter domain.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell membrane Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	ATP catabolic process Inferred from electronic annotation. Source: GOC
Cellular_component	ATP-binding cassette (ABC) transporter complex Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW putrescine-importing ATPase activity Inferred from electronic annotation. Source: InterPro spermidine-importing ATPase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 417	417	Spermidine/putrescine import ATP-binding protein PotA		PRO_0000286190
Regions
	Domain	5 – 308	304	ABC transporter
	Nucleotide binding	37 – 44	8	ATP Potential
	Region	105 – 177	73	Insert

Sequences

	Sequence		Length	Mass (Da)
	Q2NK31 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 6EBD58D36DE4C5D8	FASTA	417	47,776
	10 20 30 40 50 60 MKTLIILKDL TKVFDNQLIL RGINLEIKQN EFVTLLGPSG CGKTTILRIL GGFENPSSGE 70 80 90 100 110 120 VLFQGKSILN VAAHKRPINT VFQKYALFPH LNVFENVAFG LRLKDFNSKI KDNLDINETH 130 140 150 160 170 180 YKTSKANLSK TFHQELTKDL AQEKTTQITN NFNNQIEALT KDFEAKQTAL KCKKINKKEQ 190 200 210 220 230 240 EEQIQKEVLK YIKIMGLQGL EKRTIEQLSG GQQQRVAIAR ALINKPQVLL LDEPLSNLDL 250 260 270 280 290 300 KLKQEMQYEL KEIQKNSGIT FLFVTHDQEE AFTMSDKVVV MNNGEIQQIG SPEDIYNEPA 310 320 330 340 350 360 NRFVAQFVGE SNLIKGVMKD DFLVHFDNQT FNCVDKGFRK EENVDIVIRP EDIDIVYQGK 370 380 390 400 410 GLITGIVQSI IFKGVHWEID VKTAQRTYII HTTDHVELNK KIDITFNPED IHVMEIW

References

[1]

"Living with genome instability: the adaptation of phytoplasmas to diverse environments of their insect and plant hosts." Bai X., Zhang J., Ewing A., Miller S.A., Jancso Radek A., Shevchenko D.V., Tsukerman K., Walunas T., Lapidus A., Campbell J.W., Hogenhout S.A. J. Bacteriol. 188:3682-3696(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AYWB.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000061 Genomic DNA. Translation: ABC65212.1.
RefSeq	YP_456291.1. NC_007716.1.
3D structure databases
ProteinModelPortal	Q2NK31.
ModBase	Search...
Protein-protein interaction databases
STRING	322098.AYWB_095.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABC65212; ABC65212; AYWB_095.
GeneID	3866049.
KEGG	ayw:AYWB_095.
PATRIC	21004106. VBIAstYel136969_0114.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3842.
KO	K11072.
OMA	KENIHIM.
ProtClustDB	CLSK343826.
Enzyme and pathway databases
BioCyc	CPHY322098:GJ7E-98-MONOMER.
Family and domain databases
InterPro	IPR003593. AAA+_ATPase. IPR017879. ABC_Sperm/Put_ATP-bd_PotA. IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR008995. Mo/tungstate-bd_C_term_dom. IPR027417. P-loop_NTPase. IPR013611. Transp-assoc_OB_typ2. [Graphical view]
Pfam	PF00005. ABC_tran. 1 hit. PF08402. TOBE_2. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
SUPFAM	SSF50331. MOP_like. 1 hit. SSF52540. SSF52540. 1 hit.
PROSITE	PS00211. ABC_TRANSPORTER_1. 1 hit. PS50893. ABC_TRANSPORTER_2. 1 hit. PS51305. POTA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

POTA_AYWBP

Accession

Primary (citable) accession number: Q2NK31

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	February 7, 2006
Last modified:	May 29, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program