Reviewed, UniProtKB/Swiss-Prot Q2NK04 (PYRG_AYWBP)

Names and origin

Protein names

Recommended name:
    CTP synthase
    EC=6.3.4.2
Alternative name(s):
    UTP--ammonia ligase
    CTP synthetase

Gene names

Name:	pyrG
Ordered Locus Names:	AYWB_122

Organism

Aster yellows witches'-broom phytoplasma (strain AYWB) [Complete proteome] [HAMAP]

Taxonomic identifier

322098 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Acholeplasmatales › Acholeplasmataceae › Candidatus Phytoplasma › Candidatus Phytoplasma asteris

Protein attributes

Sequence length	536 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen By similarity.
Catalytic activity	ATP + UTP + NH3 = ADP + phosphate + CTP. HAMAP MF_01227
Enzyme regulation	Allosterically activated by GTP, when glutamine is the substrate. Inhibited by CTP By similarity.
Pathway	Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2. HAMAP MF_01227
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the CTP synthase family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW CTP synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 536	536	CTP synthase HAMAP MF_01227		PRO_0000266056
Regions
	Domain	306 – 536	231	Glutamine amidotransferase type-1
	Region	1 – 260	260	Aminator domain HAMAP MF_01227
Sites
	Active site	388	1	Nucleophile By similarity
	Active site	512	1	By similarity
	Active site	514	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2NK04-1 [UniParc]. Last modified December 12, 2006. Version 2. Checksum: F2C6E114400F2ADE	FASTA	536	60,798
	10 20 30 40 50 60 MNNKDLKTKF IFITGGVVSS LGKGITAASI GQILKNRGLK VSIQKLDPYI NVDPGTMSPY 70 80 90 100 110 120 QHGEVFVTDD GAETDLDLGH YERFLDENMS KKSNVTAGQI YQSVINKERE GKYLGKTVQV 130 140 150 160 170 180 IPHITEEIKQ KLTDAALFHK SDVVIVEIGG TVGDIESSPF LEAIRQVRFD FGYRNVLYLH 190 200 210 220 230 240 TTLVPYLKKT KEIKTKPTQH SFKELRALGI QPQILVLRSE VPINQETKNK ISALCDINSQ 250 260 270 280 290 300 AIFEALDVDI LYQMILNLNH QGIDDFILQH FKLTNFPTAD LQAWQQLITR IQNLEKKVVI 310 320 330 340 350 360 ALVGKYIVLH DAYLSIVEAL KHASYQYNCK LEIKWIDAEK VTSDNISSFF EDCDGILVPY 370 380 390 400 410 420 GFGNRAIEGK ILAINYARTN NIPFFGICLG MQLAVIEYAR NVLHLQDANS LEVDEKTPHP 430 440 450 460 470 480 VITKKIVDIN LGGTLRLGSY SCHLKANTKS KAIYNQEIIY ERHRHRFEMN PHYVSLFEKN 490 500 510 520 530 NDFVVSGINP EQNLCEIVEL KSHPWFIAVQ FHPEFLSRPL KPHPLFKGFV EASLLN

References

[1]

"Living with genome instability: the adaptation of phytoplasmas to diverse environments of their insect and plant hosts." Bai X., Zhang J., Ewing A., Miller S.A., Jancso Radek A., Shevchenko D.V., Tsukerman K., Walunas T., Lapidus A., Campbell J.W., Hogenhout S.A. J. Bacteriol. 188:3682-3696(2006) [PubMed: 16672622] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000061 Genomic DNA. Translation: ABC65239.1. Different initiation.
RefSeq	YP_456318.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3865884.
GenomeReviews	Gene locus AYWB_122 in contig CP000061_GR.
KEGG	ayw:AYWB_122.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2NK04.
Family and domain databases
HAMAP	MF_01227. [Tree]
InterPro	IPR004468. CTP_synthase. IPR017456. CTP_synthase_N. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11550. PyrG_synth. 1 hit.
Pfam	PF06418. CTP_synth_N. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00337. PyrG. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRG_AYWBP

Accession

Primary (citable) accession number: Q2NK04

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	December 12, 2006
Last modified:	June 16, 2009
This is version 26 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)