Q2NJZ3 (SYFA_AYWBP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase alpha chain
EC=6.1.1.20

Alternative name(s):
Phenylalanyl-tRNA synthetase alpha chain
Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	AYWB_133

Organism

Aster yellows witches'-broom phytoplasma (strain AYWB) [Complete proteome] [HAMAP]

Taxonomic identifier

322098 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Acholeplasmatales › Acholeplasmataceae › Candidatus Phytoplasma › Candidatus Phytoplasma asteris

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity. HAMAP MF_00281
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm HAMAP MF_00281.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 343	343	Phenylalanine--tRNA ligase alpha chain HAMAP MF_00281		PRO_1000006797
Sites
	Metal binding	256	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2NJZ3 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: D1630DFA97EFBF6A	FASTA	343	39,875
	10 20 30 40 50 60 MQTKIKKLIA QFHTSLQKNK YDLDKLMILD KEFLGKKGIL FELTQELKNL PHAQKSVAGK 70 80 90 100 110 120 LINFLKQEII FTLQKEKETL KRNQLNAKIL QEEIDPTLPA FNFCQGSTHP LNQIIEKIED 130 140 150 160 170 180 LFLSLGYEIK EGNEIETLFY NFEMLNMGKG HPAREMQDSF YIDSQKLLRT HTSNIQVKEM 190 200 210 220 230 240 KARQGKPLKI ISSGKVYRKD DDDATHSHQF MQLEGLVIDK NINFSVLKET LLLITKELFG 250 260 270 280 290 300 NSQEIHLRPS YFPFTEPSIE VDLVITKKDG TKEYLEILGA GLVHPQVLKN ANYDPEKYQG 310 320 330 340 FAFGIGIERI AMIKYQIENI RYFYANDIRF LKQFARNADN ENY

References

[1]

"Living with genome instability: the adaptation of phytoplasmas to diverse environments of their insect and plant hosts." Bai X., Zhang J., Ewing A., Miller S.A., Jancso Radek A., Shevchenko D.V., Tsukerman K., Walunas T., Lapidus A., Campbell J.W., Hogenhout S.A. J. Bacteriol. 188:3682-3696(2006) [PubMed: 16672622] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AYWB.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000061 Genomic DNA. Translation: ABC65250.1.
RefSeq	YP_456329.1. NC_007716.1.
3D structure databases
ProteinModelPortal	Q2NJZ3.
SMR	Q2NJZ3. Positions 86-334.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2NJZ3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3865826.
GenomeReviews	Gene locus AYWB_133 in contig CP000061_GR.
KEGG	ayw:AYWB_133.
PATRIC	21004202. VBIAstYel136969_0158.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0016.
HOGENOM	HBG284353.
OMA	FRASYFP.
PhylomeDB	Q2NJZ3.
ProtClustDB	PRK00488.
Enzyme and pathway databases
BioCyc	AYEL322098:AYWB_133-MONOMER.
Family and domain databases
HAMAP	MF_00281. Phe_tRNA_synth_alpha1. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_synth_II_N. IPR022911. Phe_tRNA_synth_alpha1_bac. IPR002319. Phenylalanyl-tRNA_Synthase. IPR010978. tRNA-bd_arm. [Graphical view]
KO	K01889.
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
SUPFAM	SSF46589. tRNA_binding_arm. 1 hit.
TIGRFAMs	TIGR00468. PheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_AYWBP

Accession

Primary (citable) accession number: Q2NJZ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 7, 2006
Last modified:	January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program