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Q2NJZ3 (SYFA_AYWBP) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phenylalanine--tRNA ligase alpha chain

EC=6.1.1.20
Alternative name(s):
Phenylalanyl-tRNA synthetase alpha chain
Short name=PheRS
Gene names
Name:pheS
Ordered Locus Names:AYWB_133
OrganismAster yellows witches'-broom phytoplasma (strain AYWB) [Complete proteome] [HAMAP]
Taxonomic identifier322098 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesAcholeplasmatalesAcholeplasmataceaeCandidatus PhytoplasmaCandidatus Phytoplasma asteris

Protein attributes

Sequence length343 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281

Cofactor

Binds 2 magnesium ions per tetramer By similarity. HAMAP MF_00281

Subunit structure

Tetramer of two alpha and two beta chains By similarity.

Subcellular location

Cytoplasm HAMAP MF_00281.

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processphenylalanyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

phenylalanine-tRNA ligase activity

Inferred from electronic annotation. Source: EC

tRNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 343343Phenylalanine--tRNA ligase alpha chain HAMAP MF_00281
PRO_1000006797

Sites

Metal binding2561Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2NJZ3 [UniParc].

Last modified February 7, 2006. Version 1.
Checksum: D1630DFA97EFBF6A

FASTA34339,875
        10         20         30         40         50         60 
MQTKIKKLIA QFHTSLQKNK YDLDKLMILD KEFLGKKGIL FELTQELKNL PHAQKSVAGK 

        70         80         90        100        110        120 
LINFLKQEII FTLQKEKETL KRNQLNAKIL QEEIDPTLPA FNFCQGSTHP LNQIIEKIED 

       130        140        150        160        170        180 
LFLSLGYEIK EGNEIETLFY NFEMLNMGKG HPAREMQDSF YIDSQKLLRT HTSNIQVKEM 

       190        200        210        220        230        240 
KARQGKPLKI ISSGKVYRKD DDDATHSHQF MQLEGLVIDK NINFSVLKET LLLITKELFG 

       250        260        270        280        290        300 
NSQEIHLRPS YFPFTEPSIE VDLVITKKDG TKEYLEILGA GLVHPQVLKN ANYDPEKYQG 

       310        320        330        340 
FAFGIGIERI AMIKYQIENI RYFYANDIRF LKQFARNADN ENY 

« Hide

References

[1]"Living with genome instability: the adaptation of phytoplasmas to diverse environments of their insect and plant hosts."
Bai X., Zhang J., Ewing A., Miller S.A., Jancso Radek A., Shevchenko D.V., Tsukerman K., Walunas T., Lapidus A., Campbell J.W., Hogenhout S.A.
J. Bacteriol. 188:3682-3696(2006) [PubMed: 16672622] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AYWB.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000061 Genomic DNA. Translation: ABC65250.1.
RefSeqYP_456329.1. NC_007716.1.

3D structure databases

ProteinModelPortalQ2NJZ3.
SMRQ2NJZ3. Positions 86-334.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ2NJZ3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3865826.
GenomeReviewsGene locus AYWB_133 in contig CP000061_GR.
KEGGayw:AYWB_133.
PATRIC21004202. VBIAstYel136969_0158.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0016.
HOGENOMHBG284353.
OMAFRASYFP.
PhylomeDBQ2NJZ3.
ProtClustDBPRK00488.

Enzyme and pathway databases

BioCycAYEL322098:AYWB_133-MONOMER.

Family and domain databases

HAMAPMF_00281. Phe_tRNA_synth_alpha1.
[Tree]
InterProIPR006195. aa-tRNA-synth_II.
IPR004529. Phe-tRNA-synth_IIc_asu.
IPR004188. Phe-tRNA_synth_II_N.
IPR022911. Phe_tRNA_synth_alpha1_bac.
IPR002319. Phenylalanyl-tRNA_Synthase.
IPR010978. tRNA-bd_arm.
[Graphical view]
KOK01889.
PfamPF02912. Phe_tRNA-synt_N. 1 hit.
PF01409. tRNA-synt_2d. 1 hit.
[Graphical view]
SUPFAMSSF46589. tRNA_binding_arm. 1 hit.
TIGRFAMsTIGR00468. PheS. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYFA_AYWBP
AccessionPrimary (citable) accession number: Q2NJZ3
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 7, 2006
Last modified: January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families