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Q2NJY3

- RNC_AYWBP

UniProt

Q2NJY3 - RNC_AYWBP

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Protein

Ribonuclease 3

Gene
rnc, AYWB_143
Organism
Aster yellows witches'-broom phytoplasma (strain AYWB)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism By similarity.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.UniRule annotation

Cofactori

Mg2+ By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi45 – 451Magnesium By similarity
Active sitei49 – 491 Reviewed prediction
Metal bindingi116 – 1161Magnesium By similarity
Active sitei119 – 1191 By similarity
Metal bindingi119 – 1191Magnesium By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. ribonuclease III activity Source: UniProtKB-HAMAP
  3. rRNA binding Source: UniProtKB-KW

GO - Biological processi

  1. mRNA processing Source: UniProtKB-HAMAP
  2. rRNA catabolic process Source: InterPro
  3. rRNA processing Source: UniProtKB-HAMAP
  4. tRNA processing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

mRNA processing, rRNA processing, tRNA processing

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding, rRNA-binding

Enzyme and pathway databases

BioCyciCPHY322098:GJ7E-150-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease 3 (EC:3.1.26.3)
Alternative name(s):
Ribonuclease III
Short name:
RNase III
Gene namesi
Name:rnc
Ordered Locus Names:AYWB_143
OrganismiAster yellows witches'-broom phytoplasma (strain AYWB)
Taxonomic identifieri322098 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesAcholeplasmatalesAcholeplasmataceaeCandidatus PhytoplasmaCandidatus Phytoplasma asteris
ProteomesiUP000001934: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 237237Ribonuclease 3UniRule annotationPRO_1000075721Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi322098.AYWB_143.

Structurei

3D structure databases

ProteinModelPortaliQ2NJY3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 130127RNase IIIAdd
BLAST
Domaini154 – 22269DRBMAdd
BLAST

Sequence similaritiesi

Contains 1 RNase III domain.

Phylogenomic databases

eggNOGiCOG0571.
HOGENOMiHOG000246808.
KOiK03685.
OMAiAQKDPKT.
OrthoDBiEOG6T1WVS.

Family and domain databases

Gene3Di1.10.1520.10. 1 hit.
3.30.160.20. 1 hit.
HAMAPiMF_00104. RNase_III.
InterProiIPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view]
PANTHERiPTHR11207. PTHR11207. 1 hit.
PfamiPF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
[Graphical view]
SMARTiSM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
[Graphical view]
SUPFAMiSSF69065. SSF69065. 1 hit.
TIGRFAMsiTIGR02191. RNaseIII. 1 hit.
PROSITEiPS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2NJY3-1 [UniParc]FASTAAdd to Basket

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MKNIQILFQT LNIFPKNLVL YKQALTHSSY SNEQTPPQED NERLEFLGDA    50
IVGLLMADYL YSQSKEDEGI MTKKRAQAVC EKSLTIYAQK IELQNYLLLG 100
KGEKNKDFNA KGIMADTFEA LFGAIYLDLG YLTAKKVFHN IVLPHLAKTI 150
YIIDFKTQLQ EIVQSEKKTI QYKIVQEQGP AHSKNFVAEV YLEKNLLGTG 200
EGSTKKSAEQ KAAQQALSKV AKTKDLIKNK GVKEKEL 237
Length:237
Mass (Da):26,797
Last modified:February 7, 2006 - v1
Checksum:i9B65C152553E394B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000061 Genomic DNA. Translation: ABC65260.1.
RefSeqiWP_011412426.1. NC_007716.1.
YP_456339.1. NC_007716.1.

Genome annotation databases

EnsemblBacteriaiABC65260; ABC65260; AYWB_143.
GeneIDi3865836.
KEGGiayw:AYWB_143.
PATRICi21004222. VBIAstYel136969_0168.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000061 Genomic DNA. Translation: ABC65260.1 .
RefSeqi WP_011412426.1. NC_007716.1.
YP_456339.1. NC_007716.1.

3D structure databases

ProteinModelPortali Q2NJY3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 322098.AYWB_143.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABC65260 ; ABC65260 ; AYWB_143 .
GeneIDi 3865836.
KEGGi ayw:AYWB_143.
PATRICi 21004222. VBIAstYel136969_0168.

Phylogenomic databases

eggNOGi COG0571.
HOGENOMi HOG000246808.
KOi K03685.
OMAi AQKDPKT.
OrthoDBi EOG6T1WVS.

Enzyme and pathway databases

BioCyci CPHY322098:GJ7E-150-MONOMER.

Family and domain databases

Gene3Di 1.10.1520.10. 1 hit.
3.30.160.20. 1 hit.
HAMAPi MF_00104. RNase_III.
InterProi IPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view ]
PANTHERi PTHR11207. PTHR11207. 1 hit.
Pfami PF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
[Graphical view ]
SMARTi SM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
[Graphical view ]
SUPFAMi SSF69065. SSF69065. 1 hit.
TIGRFAMsi TIGR02191. RNaseIII. 1 hit.
PROSITEi PS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Living with genome instability: the adaptation of phytoplasmas to diverse environments of their insect and plant hosts."
    Bai X., Zhang J., Ewing A., Miller S.A., Jancso Radek A., Shevchenko D.V., Tsukerman K., Walunas T., Lapidus A., Campbell J.W., Hogenhout S.A.
    J. Bacteriol. 188:3682-3696(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AYWB.

Entry informationi

Entry nameiRNC_AYWBP
AccessioniPrimary (citable) accession number: Q2NJY3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: February 7, 2006
Last modified: September 3, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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