Reviewed, UniProtKB/Swiss-Prot Q2NJM5 (GCP_AYWBP)

Names and origin

Protein names

Recommended name:
    Probable O-sialoglycoprotein endopeptidase
      Short name=Glycoprotease
    EC=3.4.24.57

Gene names

Name:	gcp
Ordered Locus Names:	AYWB_251

Organism

Aster yellows witches'-broom phytoplasma (strain AYWB) [Complete proteome] [HAMAP]

Taxonomic identifier

322098 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Acholeplasmatales › Acholeplasmataceae › Candidatus Phytoplasma › Candidatus Phytoplasma asteris

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolysis of O-sialoglycoproteins; cleaves 31-Arg-|-Asp-32 bond in glycophorin A. Does not cleave unglycosylated proteins, desialylated glycoproteins or glycoproteins that are only N-glycosylated. HAMAP MF_01445
Cofactor	Zinc Probable.
Sequence similarities	Belongs to the peptidase M22 family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 274	274	Probable O-sialoglycoprotein endopeptidase HAMAP MF_01445		PRO_0000303259
Sites
	Metal binding	57	1	Zinc Potential
	Metal binding	61	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	Q2NJM5-1 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 0C7FF3CA77BFCF25	FASTA	274	30,226
	10 20 30 40 50 60 MTLALQQTLK EAHLTPQEID LVAVTQGPGL VGSLLVGINA ANVFAYTYKK PLLGVNHLLG 70 80 90 100 110 120 HIYSAQIEHE IKFPALVLLV SGGHTDLFYL TDHLQIKPLG TTIDDAVGEV YDKIAKNLNL 130 140 150 160 170 180 PYPGGPLIDQ LAQQGKDTYH LVRPYLKNDN LNFSFSGLKS TLVNLVMKQN LKDINIPDLC 190 200 210 220 230 240 ASFQTSVINV LCEKTKRALT KYHVKQLIVV GGVASNSGLR QKFMTSFSNL EVIFPSLQYC 250 260 270 TDQAAMIGIA AYYQNQITKA SKKYDLTAKP NLFF

References

[1]

"Living with genome instability: the adaptation of phytoplasmas to diverse environments of their insect and plant hosts." Bai X., Zhang J., Ewing A., Miller S.A., Jancso Radek A., Shevchenko D.V., Tsukerman K., Walunas T., Lapidus A., Campbell J.W., Hogenhout S.A. J. Bacteriol. 188:3682-3696(2006) [PubMed: 16672622] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000061 Genomic DNA. Translation: ABC65368.1.
RefSeq	YP_456447.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2NJM5.
Protein family/group databases
MEROPS	M22.001.
Genome annotation databases
GeneID	3865752.
GenomeReviews	Gene locus AYWB_251 in contig CP000061_GR.
KEGG	ayw:AYWB_251.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2NJM5.
OMA	MEVIGQT.
Family and domain databases
HAMAP	MF_01445. Divergent sequence. [Tree]
InterPro	IPR009180. Pept_M22_Osialgl. IPR000905. Peptidase_M22. IPR017860. Peptidase_M22_CS. IPR017861. Peptidase_M22_subgr. [Graphical view]
PANTHER	PTHR11735. Pept_M22_Osialgl. 1 hit.
Pfam	PF00814. Peptidase_M22. 1 hit. [Graphical view]
PRINTS	PR00789. OSIALOPTASE.
ProDom	PD002367. Peptidase_M22. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00329. gcp. 1 hit.
PROSITE	PS01016. GLYCOPROTEASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GCP_AYWBP

Accession

Primary (citable) accession number: Q2NJM5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	February 7, 2006
Last modified:	November 3, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)