Reviewed, UniProtKB/Swiss-Prot Q2NJG9 (TRMD_AYWBP)

Names and origin

Protein names

Recommended name:
    tRNA (guanine-N(1)-)-methyltransferase
    EC=2.1.1.31
Alternative name(s):
    M1G-methyltransferase
    tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	AYWB_307

Organism

Aster yellows witches'-broom phytoplasma (strain AYWB) [Complete proteome] [HAMAP]

Taxonomic identifier

322098 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Acholeplasmatales › Acholeplasmataceae › Candidatus Phytoplasma › Candidatus Phytoplasma asteris

Protein attributes

Sequence length	242 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(1)-methylguanine. HAMAP MF_00605
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the RNA methyltransferase trmD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 242	242	tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605		PRO_0000257390
Regions
	Region	130 – 135	6	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	111	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2NJG9-1 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 7B99E5D53F50C403	FASTA	242	27,695
	10 20 30 40 50 60 MIIEIITIFP SFFKSFCETS IIKRALEQKK VQIKIHDLRI YSSNKHNQVD DSVYGGGVGM 70 80 90 100 110 120 LLSFPPFFDC LQKIKTPQSK VILLSPQGQI FNQIHATNFA QKETHLIILC GNYEGVDARI 130 140 150 160 170 180 LQYIDAEISI GDYVLTGGEI AATVLVDAIT RLIPGVIKEQ SYLEDSHQQG LLKYPQYTRP 190 200 210 220 230 240 QNYFNHVVPT ILLSGNHAKI RSWRQKESLK KTLQKRPDLL ENKKLTLEQT KLLKEIKQEL QK

References

[1]

"Living with genome instability: the adaptation of phytoplasmas to diverse environments of their insect and plant hosts." Bai X., Zhang J., Ewing A., Miller S.A., Jancso Radek A., Shevchenko D.V., Tsukerman K., Walunas T., Lapidus A., Campbell J.W., Hogenhout S.A. J. Bacteriol. 188:3682-3696(2006) [PubMed: 16672622] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000061 Genomic DNA. Translation: ABC65424.1.
RefSeq	YP_456503.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3865901.
GenomeReviews	Gene locus AYWB_307 in contig CP000061_GR.
KEGG	ayw:AYWB_307.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2NJG9.
OMA	Q2NJG9. HRSVDDT.
Family and domain databases
HAMAP	MF_00605. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. [Graphical view]
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
ProDom	PD004978. tRNA_m1G_mtfrase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00088. trmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMD_AYWBP

Accession

Primary (citable) accession number: Q2NJG9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	February 7, 2006
Last modified:	June 16, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)