Reviewed, UniProtKB/Swiss-Prot Q2NJG7 (SYY_AYWBP)

Names and origin

Protein names

Recommended name:
    Tyrosyl-tRNA synthetase
    EC=6.1.1.1
Alternative name(s):
    Tyrosine--tRNA ligase
      Short name=TyrRS

Gene names

Name:	tyrS
Ordered Locus Names:	AYWB_309

Organism

Aster yellows witches'-broom phytoplasma (strain AYWB) [Complete proteome] [HAMAP]

Taxonomic identifier

322098 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Acholeplasmatales › Acholeplasmataceae › Candidatus Phytoplasma › Candidatus Phytoplasma asteris

Protein attributes

Sequence length	413 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity.
Catalytic activity	ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr). HAMAP MF_02006
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 1 subfamily. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tyrosyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP RNA binding Inferred from electronic annotation. Source: UniProtKB-KW tyrosine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 413	413	Tyrosyl-tRNA synthetase HAMAP MF_02006		PRO_0000234666
Regions
	Domain	347 – 413	67	S4 RNA-binding
	Motif	39 – 48	10	"HIGH" region HAMAP MF_02006
	Motif	225 – 229	5	"KMSKS" region HAMAP MF_02006
Sites
	Binding site	34	1	Tyrosine By similarity
	Binding site	164	1	Tyrosine By similarity
	Binding site	168	1	Tyrosine By similarity
	Binding site	228	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2NJG7-1 [UniParc]. Last modified May 16, 2006. Version 2. Checksum: C8C3BEA5A2B0A12F	FASTA	413	47,600
	10 20 30 40 50 60 MSFYEELKWR NLIKDCNNEI QVKELLDNNQ VKFYCGFDPT SHSLTVGHLI QITMILLMQR 70 80 90 100 110 120 QGHLPVILVG GATGLIGDPK ETEERKLLSL ENSLQNAKSI ESQLKTILLN KQVEFVNNYQ 130 140 150 160 170 180 WLSQIDIISF LRNYGKFFNI NYMLSKHVVA KRLASGISFT EFSYMILQSL DFHHLYKNHK 190 200 210 220 230 240 VRLQLGGSDQ WGNITSGLEI IRKLEKKSDA LGVSTPLLLN SDGTKFGKSE KRVLWLNPLM 250 260 270 280 290 300 TSPYEIYQYF LNVSDKEVIN YLKMLTLIPK KGILELEKKT LENPQKRLAQ KALTQSIINL 310 320 330 340 350 360 IHSSDILQEC IKTNQILFSN AKKESFQEKD FILLQKTLFC HSIKEDILLV DALVQTKLAT 370 380 390 400 410 SKSEAREFIK DNTIKLFNQK IKSLDFIITK ENTLFGKYIL LKKGKKNNAL IVF

References

[1]

"Living with genome instability: the adaptation of phytoplasmas to diverse environments of their insect and plant hosts." Bai X., Zhang J., Ewing A., Miller S.A., Jancso Radek A., Shevchenko D.V., Tsukerman K., Walunas T., Lapidus A., Campbell J.W., Hogenhout S.A. J. Bacteriol. 188:3682-3696(2006) [PubMed: 16672622] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000061 Genomic DNA. Translation: ABC65426.1. Different initiation.
RefSeq	YP_456505.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2NJG7.
Genome annotation databases
GeneID	3865903.
GenomeReviews	Gene locus AYWB_309 in contig CP000061_GR.
KEGG	ayw:AYWB_309.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2NJG7.
Family and domain databases
HAMAP	MF_02006. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ib. IPR014729. Rossmann-like_a/b/a_fold. IPR002942. S4_RNA_bd. IPR002307. Tyr-tRNA-synth_Ib_bac/mito. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11766. Tyr_tRNA-synt_1b. 1 hit.
Pfam	PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01040. TRNASYNTHTYR.
SMART	SM00363. S4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00234. tyrS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. PS50889. S4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYY_AYWBP

Accession

Primary (citable) accession number: Q2NJG7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	May 16, 2006
Last modified:	September 1, 2009
This is version 23 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)