Reviewed, UniProtKB/Swiss-Prot Q2NJ60 (SYA_AYWBP)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	AYWB_416

Organism

Aster yellows witches'-broom phytoplasma (strain AYWB) [Complete proteome] [HAMAP]

Taxonomic identifier

322098 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Acholeplasmatales › Acholeplasmataceae › Candidatus Phytoplasma › Candidatus Phytoplasma asteris

Protein attributes

Sequence length	864 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036
Subcellular location	Cytoplasm By similarity.
Domain	The C-terminal C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 864	864	Alanyl-tRNA synthetase HAMAP MF_00036		PRO_0000347494

Sequences

	Sequence		Length	Mass (Da)
	Q2NJ60-1 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 65658154C3A15DC3	FASTA	864	100,363
	10 20 30 40 50 60 MTSFEIRQMW IKFFASKNHH IEKSFSLIPR NEDNLLWVNA GITPLKKYFD GTQTPSFRRI 70 80 90 100 110 120 TNVQKCIRTK DIKNVGKTSR HHTFFEMLGN FSIGNYFKKE AIHYAFELLT SPKWFGFPLE 130 140 150 160 170 180 KLYITYFFQD QDTYQYWLDL GVNKNHLIHL KDNFWQIGPG PSGPCTEIFF DRGKTFDPRN 190 200 210 220 230 240 KELIIEDLEN DRFIEIWNIV FSQYNCDPKL PIEKYQELPS KNIDTGAGLE RLACILQNTK 250 260 270 280 290 300 TNFETDLFFP LIKALEKMTQ ITYTGQESFK IIADHLKTLV FAINDGAVLT NEKRGYVLKK 310 320 330 340 350 360 LLRRAANEGK KLGLEKPFLY KLVPPTVAMM KDFYKELCTN QEMIAKVLLQ QENIFEKTLK 370 380 390 400 410 420 TAEKTFLQHL TQNTLSGKNF FKLYDTYGIP ENLILDYAKK KNITTDYQKF QELLHEQQNL 430 440 450 460 470 480 SKKNQTSQTN MNKQEEAFLH FLTPSEFIGY TNFACKTKVI KVFDEGIVLE KTPFYANMGG 490 500 510 520 530 540 QIEDEGWIDN NKVTKITKLP NGQILHEFKG NFCEGQEVYA CIDKTKRKQI SYHHTATHLL 550 560 570 580 590 600 EVVLQKQLGN HIKKQGSSVG FSSLRYDFNH FEKITPQTLL QIEKEVNQLI QKRVPVKIEQ 610 620 630 640 650 660 LSIQDAQKKY ATLLEQNQKA KYKDKVRIVN IDTFSVDLCG GTHATNTKDL EHFTILSYES 670 680 690 700 710 720 ISSGIYRIEA VCNKNCQESL NAKLAPYQNE LHQLTQKAKS LQTQNLDFEI KKFPPITKSY 730 740 750 760 770 780 QDIINYQKHI KTQQQALVLF EKKVLEHHQK KMIQAESNFL PPQINKKMML TIKEEKPLEV 790 800 810 820 830 840 IKFFMNHIFY KYHLEVLFLS YVQPEKIVFL CQSKTLHAGN LIKEGVSLAC GSGGGNASLA 850 860 QGGTKKTQNL EKMLNFVKNK LKIN

References

[1]

"Living with genome instability: the adaptation of phytoplasmas to diverse environments of their insect and plant hosts." Bai X., Zhang J., Ewing A., Miller S.A., Jancso Radek A., Shevchenko D.V., Tsukerman K., Walunas T., Lapidus A., Campbell J.W., Hogenhout S.A. J. Bacteriol. 188:3682-3696(2006) [PubMed: 16672622] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000061 Genomic DNA. Translation: ABC65533.1.
RefSeq	YP_456612.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2NJ60.
Genome annotation databases
GeneID	3865575.
GenomeReviews	Gene locus AYWB_416 in contig CP000061_GR.
KEGG	ayw:AYWB_416.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2NJ60.
OMA	GHTIEPS
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_cons-reg. IPR018164. Ala-tRNA-synth_IIc_N. IPR003156. Pesterase_DHHA1. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_AYWBP

Accession

Primary (citable) accession number: Q2NJ60

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	February 7, 2006
Last modified:	November 24, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)