ID   TYSY_AYWBP              Reviewed;         288 AA.
AC   Q2NJ46;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   07-FEB-2006, sequence version 1.
DT   16-JUN-2009, entry version 29.
DE   RecName: Full=Thymidylate synthase;
DE            Short=TS;
DE            Short=TSase;
DE            EC=2.1.1.45;
GN   Name=thyA; OrderedLocusNames=AYWB_430;
OS   Aster yellows witches'-broom phytoplasma (strain AYWB).
OC   Bacteria; Tenericutes; Mollicutes; Acholeplasmatales;
OC   Acholeplasmataceae; Candidatus Phytoplasma;
OC   Candidatus Phytoplasma asteris.
OX   NCBI_TaxID=322098;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16672622; DOI=10.1128/JB.188.10.3682-3696.2006;
RA   Bai X., Zhang J., Ewing A., Miller S.A., Jancso Radek A.,
RA   Shevchenko D.V., Tsukerman K., Walunas T., Lapidus A., Campbell J.W.,
RA   Hogenhout S.A.;
RT   "Living with genome instability: the adaptation of phytoplasmas to
RT   diverse environments of their insect and plant hosts.";
RL   J. Bacteriol. 188:3682-3696(2006).
CC   -!- FUNCTION: Provides the sole de novo source of dTMP for DNA
CC       biosynthesis (By similarity).
CC   -!- CATALYTIC ACTIVITY: 5,10-methylenetetrahydrofolate + dUMP =
CC       dihydrofolate + dTMP.
CC   -!- PATHWAY: Pyrimidine metabolism; dTTP biosynthesis.
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the thymidylate synthase family. Bacterial-
CC       type thyA subfamily.
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DR   EMBL; CP000061; ABC65547.1; -; Genomic_DNA.
DR   RefSeq; YP_456626.1; -.
DR   GeneID; 3865589; -.
DR   GenomeReviews; CP000061_GR; AYWB_430.
DR   KEGG; ayw:AYWB_430; -.
DR   HOGENOM; Q2NJ46; -.
DR   OMA; Q2NJ46; LGPVYGH.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004799; F:thymidylate synthase activity; IEA:HAMAP.
DR   GO; GO:0006231; P:dTMP biosynthetic process; IEA:HAMAP.
DR   HAMAP; MF_00008; -; 1.
DR   InterPro; IPR000398; Thymidylat_synth_C.
DR   Gene3D; G3DSA:3.30.572.10; Thymidylat_synth_C; 1.
DR   PANTHER; PTHR11549:SF2; Thymidylat_synth_C; 1.
DR   Pfam; PF00303; Thymidylat_synt; 1.
DR   PRINTS; PR00108; THYMDSNTHASE.
DR   ProDom; PD001180; Thymidylat_synth; 1.
DR   TIGRFAMs; TIGR03284; thym_sym; 1.
DR   PROSITE; PS00091; THYMIDYLATE_SYNTHASE; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; Methyltransferase;
KW   Nucleotide biosynthesis; Transferase.
FT   CHAIN         1    288       Thymidylate synthase.
FT                                /FTId=PRO_1000000573.
FT   ACT_SITE    170    170       By similarity.
SQ   SEQUENCE   288 AA;  33786 MW;  962B5E8B04338454 CRC64;
     MNTYLDLCRF IFQKGSFRKD RTNTGTKSVF GYQMRFNLEE GFPLLTTKKM NLRSIIHELL
     WFLKGDTNIF YLVQNNVNIW NEWPYQKYQQ SDFFQNETLK EFVKKIKNDP LFATKHGNLG
     PVYGKQWRDF NGVDQIKFLI SEIKANPNSR RLILNSWNPS LLNQMALPPC HVLIQFYVHQ
     GKLSLQLYQR SGDVFLGIPF NIASYSLLLM MVAQVTNLQP YEFIHTLGDA HIYSNHITQV
     QTQIQRTPKK LPQMILNPDI KNIDDFKFID FILKNYQCDG ILKGDIAV
//