Reviewed, UniProtKB/Swiss-Prot Q2NJ46 (TYSY_AYWBP)

Names and origin

Protein names

Recommended name:
    Thymidylate synthase
      Short name=TS
      Short name=TSase
    EC=2.1.1.45

Gene names

Name:	thyA
Ordered Locus Names:	AYWB_430

Organism

Aster yellows witches'-broom phytoplasma (strain AYWB) [Complete proteome] [HAMAP]

Taxonomic identifier

322098 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Acholeplasmatales › Acholeplasmataceae › Candidatus Phytoplasma › Candidatus Phytoplasma asteris

Protein attributes

Sequence length	288 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP MF_00008
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008
Subunit structure	Homodimer By similarity. HAMAP MF_00008
Subcellular location	Cytoplasm By similarity HAMAP MF_00008.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type thyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thymidylate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 288	288	Thymidylate synthase HAMAP MF_00008		PRO_1000000573
Sites
	Active site	170	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2NJ46-1 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 962B5E8B04338454	FASTA	288	33,786
	10 20 30 40 50 60 MNTYLDLCRF IFQKGSFRKD RTNTGTKSVF GYQMRFNLEE GFPLLTTKKM NLRSIIHELL 70 80 90 100 110 120 WFLKGDTNIF YLVQNNVNIW NEWPYQKYQQ SDFFQNETLK EFVKKIKNDP LFATKHGNLG 130 140 150 160 170 180 PVYGKQWRDF NGVDQIKFLI SEIKANPNSR RLILNSWNPS LLNQMALPPC HVLIQFYVHQ 190 200 210 220 230 240 GKLSLQLYQR SGDVFLGIPF NIASYSLLLM MVAQVTNLQP YEFIHTLGDA HIYSNHITQV 250 260 270 280 QTQIQRTPKK LPQMILNPDI KNIDDFKFID FILKNYQCDG ILKGDIAV

References

[1]

"Living with genome instability: the adaptation of phytoplasmas to diverse environments of their insect and plant hosts." Bai X., Zhang J., Ewing A., Miller S.A., Jancso Radek A., Shevchenko D.V., Tsukerman K., Walunas T., Lapidus A., Campbell J.W., Hogenhout S.A. J. Bacteriol. 188:3682-3696(2006) [PubMed: 16672622] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000061 Genomic DNA. Translation: ABC65547.1.
RefSeq	YP_456626.1.
3D structure databases
HSSP	HSSP built from PDB template 2G8O based on UniProtKB P0A884.
SMR	Q2NJ46. Positions 3-288.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2NJ46.
Genome annotation databases
GeneID	3865589.
GenomeReviews	Gene locus AYWB_430 in contig CP000061_GR.
KEGG	ayw:AYWB_430.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0207.
HOGENOM	HBG588098.
OMA	AWHPANA.
PhylomeDB	Q2NJ46.
Family and domain databases
HAMAP	MF_00008. Thymidy_synth_bact. [Tree]
InterPro	IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS. [Graphical view]
Gene3D	G3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
PANTHER	PTHR11549:SF2. Thymidylat_synth_C. 1 hit.
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
TIGRFAMs	TIGR03284. thym_sym. 1 hit.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_AYWBP

Accession

Primary (citable) accession number: Q2NJ46

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 7, 2006
Last modified:	February 9, 2010
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)