Reviewed, UniProtKB/Swiss-Prot Q2NJ30 (MIAA_AYWBP)

Names and origin

Protein names

Recommended name:
    tRNA Delta(2)-isopentenylpyrophosphate transferase
      Short name=IPP transferase
    EC=2.5.1.8
Alternative name(s):
    Isopentenyl-diphosphate:tRNA isopentenyltransferase
      Short name=IPTase
      Short name=IPPT
    Dimethylallyl diphosphate:tRNA dimethylallyltransferase
      Short name=DMAPP:tRNA dimethylallyltransferase
      Short name=DMATase

Gene names

Name:	miaA
Ordered Locus Names:	AYWB_446

Organism

Aster yellows witches'-broom phytoplasma (strain AYWB) [Complete proteome] [HAMAP]

Taxonomic identifier

322098 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Acholeplasmatales › Acholeplasmataceae › Candidatus Phytoplasma › Candidatus Phytoplasma asteris

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i6A) By similarity.
Catalytic activity	Isopentenyl diphosphate + tRNA = diphosphate + tRNA containing 6-isopentenyladenosine. HAMAP MF_00185
Cofactor	Magnesium By similarity.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the IPP transferase family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	ATP-binding Magnesium Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP nucleotidyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW tRNA isopentenyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 291	291	tRNA Delta(2)-isopentenylpyrophosphate transferase		PRO_1000020561
Regions
	Nucleotide binding	9 – 16	8	ATP Potential
	Region	11 – 16	6	Substrate binding By similarity
	Region	34 – 37	4	Interaction with substrate tRNA By similarity
Sites
	Site	96	1	Interaction with substrate tRNA By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2NJ30-1 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 4DCC40BC1C2582D5	FASTA	291	33,524
	10 20 30 40 50 60 MKKVIAITGP TASGKTSLSI KIAKKFNLEI INCDSLQMYQ KYDIGTAKIT IEEAQGIKHH 70 80 90 100 110 120 LLDFLTPGTN YNIYYFQKDA RKKIEEMPLP LFVGGSGLYL KSVLFDYELT PKSLFLPPIS 130 140 150 160 170 180 LTAIENMVDF IKKKDPQLIA NLDLKNPRRI LSAYQDLLSG TLRSQKNKKH NPLYSSLIFY 190 200 210 220 230 240 LDIDRQILKK RVILRLEQML KKGFIEEVNQ IQTFFPNANF NIIGYREIKV LLEGKITLDQ 250 260 270 280 290 AKTLIIQKTM QYAKRQKTWF KNQIKPMILD ALSPDLEKTT IGLINNFLKT D

References

[1]

"Living with genome instability: the adaptation of phytoplasmas to diverse environments of their insect and plant hosts." Bai X., Zhang J., Ewing A., Miller S.A., Jancso Radek A., Shevchenko D.V., Tsukerman K., Walunas T., Lapidus A., Campbell J.W., Hogenhout S.A. J. Bacteriol. 188:3682-3696(2006) [PubMed: 16672622] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000061 Genomic DNA. Translation: ABC65563.1.
RefSeq	YP_456642.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3865779.
GenomeReviews	Gene locus AYWB_446 in contig CP000061_GR.
KEGG	ayw:AYWB_446.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2NJ30.
OMA	Q2NJ30. QLPYTVA.
Family and domain databases
HAMAP	MF_00185. [Tree]
InterPro	IPR002627. IPPT. IPR018022. tRNA_delta_PyrP_Trfase. [Graphical view]
PANTHER	PTHR11088. IPPT. 1 hit.
Pfam	PF01715. IPPT. 1 hit. [Graphical view]
ProDom	PD004674. IPPT. 1 hit. PD005388. IPPtrans_like. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00174. miaA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MIAA_AYWBP

Accession

Primary (citable) accession number: Q2NJ30

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 7, 2006
Last modified:	June 16, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)