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Q2NJ20

- EFTU_AYWBP

UniProt

Q2NJ20 - EFTU_AYWBP

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Protein

Elongation factor Tu

Gene

tuf

Organism
Aster yellows witches'-broom phytoplasma (strain AYWB)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi19 – 268GTPUniRule annotation
Nucleotide bindingi81 – 855GTPUniRule annotation
Nucleotide bindingi136 – 1394GTPUniRule annotation

GO - Molecular functioni

  1. GTPase activity Source: InterPro
  2. GTP binding Source: UniProtKB-HAMAP
  3. translation elongation factor activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCPHY322098:GJ7E-480-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor TuUniRule annotation
Short name:
EF-TuUniRule annotation
Gene namesi
Name:tufUniRule annotation
Ordered Locus Names:AYWB_456
OrganismiAster yellows witches'-broom phytoplasma (strain AYWB)
Taxonomic identifieri322098 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesAcholeplasmatalesAcholeplasmataceaeCandidatus PhytoplasmaCandidatus Phytoplasma asteris
ProteomesiUP000001934: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 394394Elongation factor TuPRO_1000015602Add
BLAST

Proteomic databases

PRIDEiQ2NJ20.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi322098.AYWB_456.

Structurei

3D structure databases

ProteinModelPortaliQ2NJ20.
SMRiQ2NJ20. Positions 2-394.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 204195tr-type GAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni19 – 268G1By similarity
Regioni60 – 645G2By similarity
Regioni81 – 844G3By similarity
Regioni136 – 1394G4By similarity
Regioni174 – 1763G5By similarity

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0050.
HOGENOMiHOG000229290.
KOiK02358.
OMAiGTEMCMP.
OrthoDBiEOG6R5C6X.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_B. EF_Tu_B.
InterProiIPR000795. EF_GTP-bd_dom.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004161. Transl_elong_EFTu/EF1A_2.
IPR004541. Transl_elong_EFTu/EF1A_bac/org.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00485. EF-Tu. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2NJ20-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MANEKFIRNK THLNVGTIGH VDHGKTTLTA AITQVLSARG LAKSRAYDQI
60 70 80 90 100
DNAPEERERG ITIKTSHVEY ETEKRHYAHV DCPGHADYVK NMITGAAQMD
110 120 130 140 150
AAILVVSGAD SVMPQTREHI LLARQVGVPK IVVFLNKCDL SPDEQILELV
160 170 180 190 200
EMEVRELLSQ YDFPGDDIPV IRGSALKALE GDAHYVAQVN KLIETLDTYI
210 220 230 240 250
EDPVREVDKP FLMPVEDVFT ITGRGTVVTG RVERGQVKAG DEVEIVGLKE
260 270 280 290 300
TRKTIVTAVE MFKKDLEFAQ AGDNVGALLR GINREDVQRG QVLAKPGSVK
310 320 330 340 350
PHSKFIAQVY VLTKEEGGRH TAFFSQYRPQ FYFRTTDITG VVELQGDVKM
360 370 380 390
VMPGDNAELV VTLNNPIAIE EGTKFSIREG GKTVGAGSVS KLLN
Length:394
Mass (Da):43,408
Last modified:February 7, 2006 - v1
Checksum:i9BFB980A563939F2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000061 Genomic DNA. Translation: ABC65573.1.
RefSeqiYP_456652.1. NC_007716.1.

Genome annotation databases

EnsemblBacteriaiABC65573; ABC65573; AYWB_456.
GeneIDi3865809.
KEGGiayw:AYWB_456.
PATRICi21004946. VBIAstYel136969_0514.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000061 Genomic DNA. Translation: ABC65573.1 .
RefSeqi YP_456652.1. NC_007716.1.

3D structure databases

ProteinModelPortali Q2NJ20.
SMRi Q2NJ20. Positions 2-394.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 322098.AYWB_456.

Proteomic databases

PRIDEi Q2NJ20.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABC65573 ; ABC65573 ; AYWB_456 .
GeneIDi 3865809.
KEGGi ayw:AYWB_456.
PATRICi 21004946. VBIAstYel136969_0514.

Phylogenomic databases

eggNOGi COG0050.
HOGENOMi HOG000229290.
KOi K02358.
OMAi GTEMCMP.
OrthoDBi EOG6R5C6X.

Enzyme and pathway databases

BioCyci CPHY322098:GJ7E-480-MONOMER.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
HAMAPi MF_00118_B. EF_Tu_B.
InterProi IPR000795. EF_GTP-bd_dom.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004161. Transl_elong_EFTu/EF1A_2.
IPR004541. Transl_elong_EFTu/EF1A_bac/org.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view ]
Pfami PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view ]
PRINTSi PR00315. ELONGATNFCT.
SUPFAMi SSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00485. EF-Tu. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEi PS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Living with genome instability: the adaptation of phytoplasmas to diverse environments of their insect and plant hosts."
    Bai X., Zhang J., Ewing A., Miller S.A., Jancso Radek A., Shevchenko D.V., Tsukerman K., Walunas T., Lapidus A., Campbell J.W., Hogenhout S.A.
    J. Bacteriol. 188:3682-3696(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AYWB.

Entry informationi

Entry nameiEFTU_AYWBP
AccessioniPrimary (citable) accession number: Q2NJ20
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 7, 2006
Last modified: November 26, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3