Reviewed, UniProtKB/Swiss-Prot Q2NJ06 (PNP_AYWBP)

Names and origin

Protein names

Recommended name:
    Polyribonucleotide nucleotidyltransferase
    EC=2.7.7.8
Alternative name(s):
    Polynucleotide phosphorylase
      Short name=PNPase

Gene names

Name:	pnp
Ordered Locus Names:	AYWB_470

Organism

Aster yellows witches'-broom phytoplasma (strain AYWB) [Complete proteome] [HAMAP]

Taxonomic identifier

322098 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Acholeplasmatales › Acholeplasmataceae › Candidatus Phytoplasma › Candidatus Phytoplasma asteris

Protein attributes

Sequence length	715 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity.
Catalytic activity	RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate. HAMAP MF_01595
Subunit structure	Homotrimer. Organized into a structure (processome or RNA degradosome) containing a number of RNA-processing enzymes By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the polyribonucleotide nucleotidyltransferase family. Contains 1 KH domain. Contains 1 S1 motif domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	RNA-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro mRNA catabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5'-exoribonuclease activity Inferred from electronic annotation. Source: InterPro RNA binding Inferred from electronic annotation. Source: UniProtKB-KW polyribonucleotide nucleotidyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 715	715	Polyribonucleotide nucleotidyltransferase HAMAP MF_01595		PRO_0000329506
Regions
	Domain	565 – 625	61	KH
	Domain	635 – 709	75	S1 motif

Sequences

	Sequence		Length	Mass (Da)
	Q2NJ06-1 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 462807B60999C430	FASTA	715	80,440
	10 20 30 40 50 60 MLKKVFETTN LKDSFQVEIG TYARNVDSSI LVRYQDTVVL TTMVFSRKPN NLDFLPLTVI 70 80 90 100 110 120 YQEKLYAAGK IPGSFLRREG RSNDHEILTS RLIDRSLRPL FPDYFQQEVQ VINTVLSLDP 130 140 150 160 170 180 DFKSELASML GSSLSLLISE IPFFEAISGV YVGKINNEFI INPTLQQLAN STLHLIVAGT 190 200 210 220 230 240 KHNVTMIEAH ANEVSEQDFL EAINFAHQYI KKLCLFQENI KQQFAPVKMT KTLHQTEQIQ 250 260 270 280 290 300 QQSFFAKNHS QVKQAILSCN SKNDLQQLKE QILDQAKQTP FFKTIDAITV FDYEAHKKHL 310 320 330 340 350 360 QITENLFQKL SKQEMRSLIL QEKIRPDKRE LEEIRTLESQ IDLLPRAHGS ALFTRGKTQS 370 380 390 400 410 420 LAAVTLGCLS ESKIIDGLSD EQNKRFMLHY NFPPFSVGAV GRYTAPSRRE IGHGTLAEKA 430 440 450 460 470 480 ISQVLPEEKD FPYTIRVVSE ILESNGSSSQ ATVCSSSLAL MASGVPLKKA VAGISVGLVF 490 500 510 520 530 540 DQETNKYVIL SDIQGLEDHV GDMDLKIAGT NKGITALQMD LKIQGIPFKI LQEAFLQAKK 550 560 570 580 590 600 GRLHILEQMN QTISQPRLEV SKYAPKVCMM QIKPEKIRDI IGSGGKIINQ IIESHDGVKI 610 620 630 640 650 660 DIEQDGRVFV MHSNLETVKK TVAFIESLIQ EIQVGTCYQA SILRFLSDKQ GKMIGAVAQV 670 680 690 700 710 CPGIEGLIHV NKMKFQKITD VLKIGETVLV KCTKINERGR IDFLLLPKKT QEKNS

References

[1]

"Living with genome instability: the adaptation of phytoplasmas to diverse environments of their insect and plant hosts." Bai X., Zhang J., Ewing A., Miller S.A., Jancso Radek A., Shevchenko D.V., Tsukerman K., Walunas T., Lapidus A., Campbell J.W., Hogenhout S.A. J. Bacteriol. 188:3682-3696(2006) [PubMed: 16672622] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000061 Genomic DNA. Translation: ABC65587.1.
RefSeq	YP_456666.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3865843.
GenomeReviews	Gene locus AYWB_470 in contig CP000061_GR.
KEGG	ayw:AYWB_470.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2NJ06.
OMA	Q2NJ06. GHGNLAK.
Family and domain databases
HAMAP	MF_01595. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR004087. KH. IPR004088. KH_type_1. IPR018111. KH_type_1_subgr. IPR012340. NA-bd_OB-fold. IPR012162. PNPase. IPR015848. PNPase_PH_RNA-bd_bac/org-type. IPR003029. Rbsml_prot_S1_RNA-bd_dom. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR11252. PNPase. 1 hit.
Pfam	PF00013. KH_1. 1 hit. PF03726. PNPase. 1 hit. PF01138. RNase_PH. 2 hits. PF03725. RNase_PH_C. 2 hits. [Graphical view]
PIRSF	PIRSF005499. PNPase. 1 hit.
SMART	SM00322. KH. 1 hit. [Graphical view]
TIGRFAMs	TIGR03591. Polynuc_phos. 1 hit.
PROSITE	PS50084. KH_TYPE_1. 1 hit. PS50126. S1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PNP_AYWBP

Accession

Primary (citable) accession number: Q2NJ06

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	February 7, 2006
Last modified:	June 16, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)