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Q2NJ06

- PNP_AYWBP

UniProt

Q2NJ06 - PNP_AYWBP

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Protein

Polyribonucleotide nucleotidyltransferase

Gene
pnp, AYWB_470
Organism
Aster yellows witches'-broom phytoplasma (strain AYWB)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi498 – 4981Magnesium By similarity
Metal bindingi504 – 5041Magnesium By similarity

GO - Molecular functioni

  1. magnesium ion binding Source: UniProtKB-HAMAP
  2. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  3. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciCPHY322098:GJ7E-494-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferase (EC:2.7.7.8)
Alternative name(s):
Polynucleotide phosphorylase
Short name:
PNPase
Gene namesi
Name:pnp
Ordered Locus Names:AYWB_470
OrganismiAster yellows witches'-broom phytoplasma (strain AYWB)
Taxonomic identifieri322098 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesAcholeplasmatalesAcholeplasmataceaeCandidatus PhytoplasmaCandidatus Phytoplasma asteris
ProteomesiUP000001934: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 715715Polyribonucleotide nucleotidyltransferaseUniRule annotationPRO_0000329506Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi322098.AYWB_470.

Structurei

3D structure databases

ProteinModelPortaliQ2NJ06.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini565 – 62561KHAdd
BLAST
Domaini635 – 70975S1 motifAdd
BLAST

Sequence similaritiesi

Contains 1 KH domain.
Contains 1 S1 motif domain.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiPRWDWVA.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2NJ06-1 [UniParc]FASTAAdd to Basket

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MLKKVFETTN LKDSFQVEIG TYARNVDSSI LVRYQDTVVL TTMVFSRKPN    50
NLDFLPLTVI YQEKLYAAGK IPGSFLRREG RSNDHEILTS RLIDRSLRPL 100
FPDYFQQEVQ VINTVLSLDP DFKSELASML GSSLSLLISE IPFFEAISGV 150
YVGKINNEFI INPTLQQLAN STLHLIVAGT KHNVTMIEAH ANEVSEQDFL 200
EAINFAHQYI KKLCLFQENI KQQFAPVKMT KTLHQTEQIQ QQSFFAKNHS 250
QVKQAILSCN SKNDLQQLKE QILDQAKQTP FFKTIDAITV FDYEAHKKHL 300
QITENLFQKL SKQEMRSLIL QEKIRPDKRE LEEIRTLESQ IDLLPRAHGS 350
ALFTRGKTQS LAAVTLGCLS ESKIIDGLSD EQNKRFMLHY NFPPFSVGAV 400
GRYTAPSRRE IGHGTLAEKA ISQVLPEEKD FPYTIRVVSE ILESNGSSSQ 450
ATVCSSSLAL MASGVPLKKA VAGISVGLVF DQETNKYVIL SDIQGLEDHV 500
GDMDLKIAGT NKGITALQMD LKIQGIPFKI LQEAFLQAKK GRLHILEQMN 550
QTISQPRLEV SKYAPKVCMM QIKPEKIRDI IGSGGKIINQ IIESHDGVKI 600
DIEQDGRVFV MHSNLETVKK TVAFIESLIQ EIQVGTCYQA SILRFLSDKQ 650
GKMIGAVAQV CPGIEGLIHV NKMKFQKITD VLKIGETVLV KCTKINERGR 700
IDFLLLPKKT QEKNS 715
Length:715
Mass (Da):80,440
Last modified:February 7, 2006 - v1
Checksum:i462807B60999C430
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000061 Genomic DNA. Translation: ABC65587.1.
RefSeqiWP_011412751.1. NC_007716.1.
YP_456666.1. NC_007716.1.

Genome annotation databases

EnsemblBacteriaiABC65587; ABC65587; AYWB_470.
GeneIDi3865843.
KEGGiayw:AYWB_470.
PATRICi21004976. VBIAstYel136969_0529.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000061 Genomic DNA. Translation: ABC65587.1 .
RefSeqi WP_011412751.1. NC_007716.1.
YP_456666.1. NC_007716.1.

3D structure databases

ProteinModelPortali Q2NJ06.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 322098.AYWB_470.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABC65587 ; ABC65587 ; AYWB_470 .
GeneIDi 3865843.
KEGGi ayw:AYWB_470.
PATRICi 21004976. VBIAstYel136969_0529.

Phylogenomic databases

eggNOGi COG1185.
HOGENOMi HOG000218326.
KOi K00962.
OMAi PRWDWVA.
OrthoDBi EOG6WT8CC.

Enzyme and pathway databases

BioCyci CPHY322098:GJ7E-494-MONOMER.

Family and domain databases

Gene3Di 2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Living with genome instability: the adaptation of phytoplasmas to diverse environments of their insect and plant hosts."
    Bai X., Zhang J., Ewing A., Miller S.A., Jancso Radek A., Shevchenko D.V., Tsukerman K., Walunas T., Lapidus A., Campbell J.W., Hogenhout S.A.
    J. Bacteriol. 188:3682-3696(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AYWB.

Entry informationi

Entry nameiPNP_AYWBP
AccessioniPrimary (citable) accession number: Q2NJ06
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: February 7, 2006
Last modified: September 3, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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