Reviewed, UniProtKB/Swiss-Prot Q2NJ01 (SYP_AYWBP)

Names and origin

Protein names

Recommended name:
    Prolyl-tRNA synthetase
    EC=6.1.1.15
Alternative name(s):
    Proline--tRNA ligase
      Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	AYWB_475

Organism

Aster yellows witches'-broom phytoplasma (strain AYWB) [Complete proteome] [HAMAP]

Taxonomic identifier

322098 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Acholeplasmatales › Acholeplasmataceae › Candidatus Phytoplasma › Candidatus Phytoplasma asteris

Protein attributes

Sequence length	474 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity.
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01571
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP proline-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 474	474	Prolyl-tRNA synthetase HAMAP MF_01571		PRO_0000249115

Sequences

	Sequence		Length	Mass (Da)
	Q2NJ01-1 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: CBF9ED2DA45716F8	FASTA	474	54,536
	10 20 30 40 50 60 MKTMKRVKTV ATRLSDFGKW YTDICLKAEL IAYSEAKGFI IYLPYGYALW ENIQKHLNCT 70 80 90 100 110 120 LQKTGHQNVY FPLVFPEKLF HKEKEHIQGF SPEAAMITTT GKKNLSEKLV IRPTSEILFS 130 140 150 160 170 180 QYYSKTITSY RDLPKLYNQW CNVVRWEKTT KPFLRGKEFL WQEGHTVHAT EQEAMQQTLS 190 200 210 220 230 240 ILDIYQKLGK DLLALPFVCG KKTETEKFAG ALITYSIEAL MHDGQALQAG TSHYLGIIFA 250 260 270 280 290 300 KSFQIQFQDC DNQKKYAHQT SWGVSTRLIG ALIMVHSDDE GLVLPPYVAP MQIVIIPLQT 310 320 330 340 350 360 QDESVKQVSE NLFSILQKNY RVHLDLQDKT AGWKFSQYEL KGVPLRIEIG KRGLENDEVT 370 380 390 400 410 420 IFQRYNFAKQ NIKIKDFPSQ IPQLFETMHN NMYQKALQHL EQNRKQATTY EEFKTYLKQG 430 440 450 460 470 GYVAMSISGT DAELQIKQET GATARVILET NLITANCPVT NKKALQTVLF ARAY

References

[1]

"Living with genome instability: the adaptation of phytoplasmas to diverse environments of their insect and plant hosts." Bai X., Zhang J., Ewing A., Miller S.A., Jancso Radek A., Shevchenko D.V., Tsukerman K., Walunas T., Lapidus A., Campbell J.W., Hogenhout S.A. J. Bacteriol. 188:3682-3696(2006) [PubMed: 16672622] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000061 Genomic DNA. Translation: ABC65592.1.
RefSeq	YP_456671.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3865854.
GenomeReviews	Gene locus AYWB_475 in contig CP000061_GR.
KEGG	ayw:AYWB_475.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2NJ01.
OMA	Q2NJ01. CIEAMMQ.
Family and domain databases
HAMAP	MF_01571. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004154. Anticodon_bd. IPR002316. Pro-tRNA-synth_IIa_cons-reg. IPR004499. Pro-tRNA-synth_IIa_pro-type. IPR016061. Pro-tRNA_synth_II_C. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.30.110.30. Pro-tRNA-synth_II_C_arc/euk. 1 hit.
PANTHER	PTHR11451:SF6. ProS_fam_I. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF09180. ProRS-C_1. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
TIGRFAMs	TIGR00408. proS_fam_I. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_AYWBP

Accession

Primary (citable) accession number: Q2NJ01

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	February 7, 2006
Last modified:	June 16, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)