ID   TRUA_AYWBP              Reviewed;         243 AA.
AC   Q2NIY3;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   07-FEB-2006, sequence version 1.
DT   16-JUN-2009, entry version 22.
DE   RecName: Full=tRNA pseudouridine synthase A;
DE            EC=5.4.99.12;
DE   AltName: Full=tRNA-uridine isomerase I;
DE   AltName: Full=tRNA pseudouridylate synthase I;
GN   Name=truA; OrderedLocusNames=AYWB_493;
OS   Aster yellows witches'-broom phytoplasma (strain AYWB).
OC   Bacteria; Tenericutes; Mollicutes; Acholeplasmatales;
OC   Acholeplasmataceae; Candidatus Phytoplasma;
OC   Candidatus Phytoplasma asteris.
OX   NCBI_TaxID=322098;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16672622; DOI=10.1128/JB.188.10.3682-3696.2006;
RA   Bai X., Zhang J., Ewing A., Miller S.A., Jancso Radek A.,
RA   Shevchenko D.V., Tsukerman K., Walunas T., Lapidus A., Campbell J.W.,
RA   Hogenhout S.A.;
RT   "Living with genome instability: the adaptation of phytoplasmas to
RT   diverse environments of their insect and plant hosts.";
RL   J. Bacteriol. 188:3682-3696(2006).
CC   -!- FUNCTION: Formation of pseudouridine at positions 38, 39 and 40 in
CC       the anticodon stem and loop of transfer RNAs (By similarity).
CC   -!- CATALYTIC ACTIVITY: tRNA uridine = tRNA pseudouridine.
CC   -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase truA
CC       family.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; CP000061; ABC65610.1; -; Genomic_DNA.
DR   RefSeq; YP_456689.1; -.
DR   GeneID; 3865912; -.
DR   GenomeReviews; CP000061_GR; AYWB_493.
DR   KEGG; ayw:AYWB_493; -.
DR   HOGENOM; Q2NIY3; -.
DR   OMA; Q2NIY3; LEIGNGR.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IEA:HAMAP.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:HAMAP.
DR   HAMAP; MF_00171; -; 1.
DR   InterPro; IPR001406; tRNA_psdUrid_synth.
DR   Gene3D; G3DSA:3.30.70.580; PseudoU_synth_1; 1.
DR   Gene3D; G3DSA:3.30.70.660; PseudoU_synth_1; 1.
DR   PANTHER; PTHR11142; PseudoU_synth_1; 1.
DR   Pfam; PF01416; PseudoU_synth_1; 2.
DR   PIRSF; PIRSF001430; tRNA_psdUrid_synth; 1.
DR   TIGRFAMs; TIGR00071; hisT_truA; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Isomerase; tRNA processing.
FT   CHAIN         1    243       tRNA pseudouridine synthase A.
FT                                /FTId=PRO_1000017038.
FT   ACT_SITE     54     54       Nucleophile (By similarity).
SQ   SEQUENCE   243 AA;  28419 MW;  5BBF5CE60D1D1558 CRC64;
     MQHFFYKLIL SYDGTFYCGY QKQPQKKTVQ QTFEKALKKM THQNIPTFAA SRTDKGVHSQ
     GQTLHFQTTF SLKPKHFQKT LNYLLPPDIR VRQMHFATPN FHARYSAKSK IYQYVFSKKP
     LNAFNHHFQI FADKFDFVKI TKALKFIEGT HNFFAFTSET QPKNFSKTIF QASLKETVHK
     YILVFHGNGF LKYMIRFLVG SLIEIGKNKF SLEQFQAMLL GNKTKKATLL APAKALVLKK
     IFY
//