Reviewed, UniProtKB/Swiss-Prot Q2NIY3 (TRUA_AYWBP)

Names and origin

Protein names

Recommended name:
    tRNA pseudouridine synthase A
    EC=5.4.99.12
Alternative name(s):
    tRNA-uridine isomerase I
    tRNA pseudouridylate synthase I

Gene names

Name:	truA
Ordered Locus Names:	AYWB_493

Organism

Aster yellows witches'-broom phytoplasma (strain AYWB) [Complete proteome] [HAMAP]

Taxonomic identifier

322098 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Acholeplasmatales › Acholeplasmataceae › Candidatus Phytoplasma › Candidatus Phytoplasma asteris

Protein attributes

Sequence length	243 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs By similarity.
Catalytic activity	tRNA uridine = tRNA pseudouridine. HAMAP MF_00171
Sequence similarities	Belongs to the tRNA pseudouridine synthase truA family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA pseudouridine synthesis Inferred from electronic annotation. Source: HAMAP
Molecular function	pseudouridine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 243	243	tRNA pseudouridine synthase A HAMAP MF_00171		PRO_1000017038
Sites
	Active site	54	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2NIY3-1 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 5BBF5CE60D1D1558	FASTA	243	28,419
	10 20 30 40 50 60 MQHFFYKLIL SYDGTFYCGY QKQPQKKTVQ QTFEKALKKM THQNIPTFAA SRTDKGVHSQ 70 80 90 100 110 120 GQTLHFQTTF SLKPKHFQKT LNYLLPPDIR VRQMHFATPN FHARYSAKSK IYQYVFSKKP 130 140 150 160 170 180 LNAFNHHFQI FADKFDFVKI TKALKFIEGT HNFFAFTSET QPKNFSKTIF QASLKETVHK 190 200 210 220 230 240 YILVFHGNGF LKYMIRFLVG SLIEIGKNKF SLEQFQAMLL GNKTKKATLL APAKALVLKK IFY

References

[1]

"Living with genome instability: the adaptation of phytoplasmas to diverse environments of their insect and plant hosts." Bai X., Zhang J., Ewing A., Miller S.A., Jancso Radek A., Shevchenko D.V., Tsukerman K., Walunas T., Lapidus A., Campbell J.W., Hogenhout S.A. J. Bacteriol. 188:3682-3696(2006) [PubMed: 16672622] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000061 Genomic DNA. Translation: ABC65610.1.
RefSeq	YP_456689.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3865912.
GenomeReviews	Gene locus AYWB_493 in contig CP000061_GR.
KEGG	ayw:AYWB_493.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2NIY3.
OMA	Q2NIY3. LEIGNGR.
Family and domain databases
HAMAP	MF_00171. [Tree]
InterPro	IPR001406. tRNA_psdUrid_synth. [Graphical view]
Gene3D	G3DSA:3.30.70.580. PseudoU_synth_1. 1 hit. G3DSA:3.30.70.660. PseudoU_synth_1. 1 hit.
PANTHER	PTHR11142. PseudoU_synth_1. 1 hit.
Pfam	PF01416. PseudoU_synth_1. 2 hits. [Graphical view]
PIRSF	PIRSF001430. tRNA_psdUrid_synth. 1 hit.
TIGRFAMs	TIGR00071. hisT_truA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRUA_AYWBP

Accession

Primary (citable) accession number: Q2NIY3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 7, 2006
Last modified:	June 16, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)