Q2NIY3 (TRUA_AYWBP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA pseudouridine synthase A
EC=5.4.99.12

Alternative name(s):
tRNA pseudouridine(38-40) synthase
tRNA pseudouridylate synthase I
tRNA-uridine isomerase I

Gene names

Name:	truA
Ordered Locus Names:	AYWB_493

Organism

Aster yellows witches'-broom phytoplasma (strain AYWB) [Complete proteome] [HAMAP]

Taxonomic identifier

322098 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Acholeplasmatales › Acholeplasmataceae › Candidatus Phytoplasma › Candidatus Phytoplasma asteris

Protein attributes

Sequence length	243 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs By similarity. HAMAP MF_00171
Catalytic activity	tRNA uridine(38-40) = tRNA pseudouridine(38-40). HAMAP MF_00171
Subunit structure	Homodimer By similarity. HAMAP MF_00171
Sequence similarities	Belongs to the tRNA pseudouridine synthase TruA family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pseudouridine synthesis Inferred from electronic annotation. Source: InterPro tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro pseudouridine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 243	243	tRNA pseudouridine synthase A HAMAP MF_00171		PRO_1000017038
Sites
	Active site	54	1	Nucleophile By similarity
	Binding site	112	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2NIY3 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 5BBF5CE60D1D1558	FASTA	243	28,419
	10 20 30 40 50 60 MQHFFYKLIL SYDGTFYCGY QKQPQKKTVQ QTFEKALKKM THQNIPTFAA SRTDKGVHSQ 70 80 90 100 110 120 GQTLHFQTTF SLKPKHFQKT LNYLLPPDIR VRQMHFATPN FHARYSAKSK IYQYVFSKKP 130 140 150 160 170 180 LNAFNHHFQI FADKFDFVKI TKALKFIEGT HNFFAFTSET QPKNFSKTIF QASLKETVHK 190 200 210 220 230 240 YILVFHGNGF LKYMIRFLVG SLIEIGKNKF SLEQFQAMLL GNKTKKATLL APAKALVLKK IFY

References

[1]

"Living with genome instability: the adaptation of phytoplasmas to diverse environments of their insect and plant hosts." Bai X., Zhang J., Ewing A., Miller S.A., Jancso Radek A., Shevchenko D.V., Tsukerman K., Walunas T., Lapidus A., Campbell J.W., Hogenhout S.A. J. Bacteriol. 188:3682-3696(2006) [PubMed: 16672622] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AYWB.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000061 Genomic DNA. Translation: ABC65610.1.
RefSeq	YP_456689.1. NC_007716.1.
3D structure databases
ProteinModelPortal	Q2NIY3.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2NIY3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3865912.
GenomeReviews	Gene locus AYWB_493 in contig CP000061_GR.
KEGG	ayw:AYWB_493.
PATRIC	21005042. VBIAstYel136969_0554.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0101.
HOGENOM	HBG621020.
OMA	RESVERP.
PhylomeDB	Q2NIY3.
ProtClustDB	PRK00021.
Enzyme and pathway databases
BioCyc	AYEL322098:AYWB_493-MONOMER.
Family and domain databases
HAMAP	MF_00171. TruA. [Tree]
InterPro	IPR020103. PsdUridine_synth_cat_dom. IPR001406. PsdUridine_synth_TruA. IPR020097. PsdUridine_synth_TruA_a/b_dom. IPR020095. PsdUridine_synth_TruA_C. IPR020094. PsdUridine_synth_TruA_N. [Graphical view]
Gene3D	G3DSA:3.30.70.580. PseudoU_synth_1. 1 hit. G3DSA:3.30.70.660. PseudoU_synth_1. 1 hit.
KO	K06173.
PANTHER	PTHR11142. PseudoU_synth_1. 1 hit.
Pfam	PF01416. PseudoU_synth_1. 2 hits. [Graphical view]
PIRSF	PIRSF001430. tRNA_psdUrid_synth. 1 hit.
SUPFAM	SSF55120. PsdUridine_synth_cat_dom. 1 hit.
TIGRFAMs	TIGR00071. HisT_truA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRUA_AYWBP

Accession

Primary (citable) accession number: Q2NIY3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 7, 2006
Last modified:	January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program