Reviewed, UniProtKB/Swiss-Prot Q2NIS7 (TPIS_AYWBP)

Names and origin

Protein names

Recommended name:
    Triosephosphate isomerase
      Short name=TIM
    EC=5.3.1.1
Alternative name(s):
    Triose-phosphate isomerase

Gene names

Name:	tpiA
Ordered Locus Names:	AYWB_549

Organism

Aster yellows witches'-broom phytoplasma (strain AYWB) [Complete proteome] [HAMAP]

Taxonomic identifier

322098 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Acholeplasmatales › Acholeplasmataceae › Candidatus Phytoplasma › Candidatus Phytoplasma asteris

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate = glycerone phosphate. HAMAP MF_00147
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_00147 Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1. HAMAP MF_00147
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the triosephosphate isomerase family.

Ontologies

Keywords
Biological process	Gluconeogenesis Glycolysis Pentose shunt
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: HAMAP glycolysis Inferred from electronic annotation. Source: HAMAP pentose-phosphate shunt Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triose-phosphate isomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 274	274	Triosephosphate isomerase HAMAP MF_00147		PRO_1000009838
Sites
	Active site	98	1	Electrophile By similarity
	Active site	170	1	Proton acceptor By similarity
	Binding site	13	1	Substrate By similarity
	Binding site	15	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2NIS7-1 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: A4BE2AE8112AA1BB	FASTA	274	30,378
	10 20 30 40 50 60 MNYKPRTKVI AGNWKMHKCK DEALEFIQKV SLQVPDQTQV QTLIFPQLTL LDPLVQLQGA 70 80 90 100 110 120 NLQVGAQNMF YETEGAFTGE VSPQNLLSLG VKHVLLGHSE RRTLFGETDQ TVNLKLLSAL 130 140 150 160 170 180 KHKLVPTVCV GESLLTKENN QTQMFLDQQL TNIFAGVPEE ALKNMIIAYE PVWAIGTGKS 190 200 210 220 230 240 ANPQDANKTI EQIREKVTAL YSFQASCAIR IIYGGSLSVA NIKSILEQPA IDGILAGKAS 250 260 270 LQTEDFLFFA QIASKQVLVS TKDIFQKNDC PFCY

References

[1]

"Living with genome instability: the adaptation of phytoplasmas to diverse environments of their insect and plant hosts." Bai X., Zhang J., Ewing A., Miller S.A., Jancso Radek A., Shevchenko D.V., Tsukerman K., Walunas T., Lapidus A., Campbell J.W., Hogenhout S.A. J. Bacteriol. 188:3682-3696(2006) [PubMed: 16672622] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000061 Genomic DNA. Translation: ABC65666.1.
RefSeq	YP_456745.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3865964.
GenomeReviews	Gene locus AYWB_549 in contig CP000061_GR.
KEGG	ayw:AYWB_549.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2NIS7.
OMA	Q2NIS7. CEETEAN.
Family and domain databases
HAMAP	MF_00147. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR000652. Triosephosphate_isomerase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR21139. Triophos_ismrse. 1 hit.
Pfam	PF00121. TIM. 1 hit. [Graphical view]
ProDom	PD001005. Triophos_ismrse. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00419. tim. 1 hit.
PROSITE	PS00171. TIM_1. 1 hit. PS51440. TIM_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TPIS_AYWBP

Accession

Primary (citable) accession number: Q2NIS7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 7, 2006
Last modified:	June 16, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)