Q2NIP6 (SYE_AYWBP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	AYWB_580

Organism

Aster yellows witches'-broom phytoplasma (strain AYWB) [Complete proteome] [HAMAP]

Taxonomic identifier

322098 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Acholeplasmatales › Acholeplasmataceae › Candidatus Phytoplasma › Candidatus Phytoplasma asteris

Protein attributes

Sequence length	449 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 449	449	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_0000237339
Regions
	Motif	10 – 20	11	"HIGH" region HAMAP MF_00022_B
	Motif	214 – 218	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	217	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2NIP6 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 9A3C1F148545480A	FASTA	449	52,265
	10 20 30 40 50 60 MKKIKVRYAP SPTGFLHIGN ARTALFNYLF AKHNQGEFII RIEDTDFSRN VKGGEASQLK 70 80 90 100 110 120 NLRWLGIDWS EGPDIQGPFG PYRQSERLAI YQKYAQKLLD QGLAYKEFQE NTTTFAIRFR 130 140 150 160 170 180 VPTNQTFSFD DLIRGKLTFQ SQEIEDWVII KSNGYPSYNF AVVIDDHLMQ ISHIFRGEEH 190 200 210 220 230 240 ITNTPKQIMI YQTFQWHLPQ FAHMTLILND NKKKLSKRDA NIMQFIEQYE KLGYLPQALF 250 260 270 280 290 300 NFLSLLGFSP LSQTEILSPQ ELINLFDVAR LNKAPAMFDT VKLDYLNNQH LRKLTPEVIA 310 320 330 340 350 360 SFIVQKKYLT LTTAPTNDKE WMTKFVSLFQ DRMNYIQQIA DFYQLFFQTK PSLSQEATIF 370 380 390 400 410 420 LQTNPQTTLI LKTFYNVFDV IVFKKDVIFN AIKQVANQND FSKKTLFMAL RIGTTCKMHG 430 440 PSIALLLELL GKKQVLKNLS YVLKQAQKF

References

[1]

"Living with genome instability: the adaptation of phytoplasmas to diverse environments of their insect and plant hosts." Bai X., Zhang J., Ewing A., Miller S.A., Jancso Radek A., Shevchenko D.V., Tsukerman K., Walunas T., Lapidus A., Campbell J.W., Hogenhout S.A. J. Bacteriol. 188:3682-3696(2006) [PubMed: 16672622] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AYWB.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000061 Genomic DNA. Translation: ABC65697.1.
RefSeq	YP_456776.1. NC_007716.1.
3D structure databases
ProteinModelPortal	Q2NIP6.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2NIP6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3866073.
GenomeReviews	Gene locus AYWB_580 in contig CP000061_GR.
KEGG	ayw:AYWB_580.
PATRIC	21005226. VBIAstYel136969_0641.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	AHMTLIL.
PhylomeDB	Q2NIP6.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	AYEL322098:AYWB_580-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_AYWBP

Accession

Primary (citable) accession number: Q2NIP6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	February 7, 2006
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program