Reviewed, UniProtKB/Swiss-Prot Q2NIN3 (SYD_AYWBP)

Names and origin

Protein names

Recommended name:
    Aspartyl-tRNA synthetase
    EC=6.1.1.12
Alternative name(s):
    Aspartate--tRNA ligase
      Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	AYWB_593

Organism

Aster yellows witches'-broom phytoplasma (strain AYWB) [Complete proteome] [HAMAP]

Taxonomic identifier

322098 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Acholeplasmatales › Acholeplasmataceae › Candidatus Phytoplasma › Candidatus Phytoplasma asteris

Protein attributes

Sequence length	576 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00044
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP aspartate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 576	576	Aspartyl-tRNA synthetase HAMAP MF_00044		PRO_0000235507

Sequences

	Sequence		Length	Mass (Da)
	Q2NIN3-1 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 3E6068732DD2AB20	FASTA	576	66,679
	10 20 30 40 50 60 MKTKYSYYNN QLTLIHQGKI VFLKGFIFRK RNLGKTLFFD LRDVSGIVQL LVKENNPQYD 70 80 90 100 110 120 KIALIKLETV VQIKGQVIER INKNPDLPTG DIEILVSHIE ILSEAQTLPL NVFQSQESLE 130 140 150 160 170 180 ETRLKYRYLD LRNPEVKHFL IQRHHITQSI RQTLLKNDFL ELETPILSKS TPEGARDYLV 190 200 210 220 230 240 PSRIYPGNFY ALPQSPQLFK QLYMIAGFER YFQVARCFRD EDLRSDRQPE FSQIDIETSF 250 260 270 280 290 300 LNQDEIMSLT EEIIVDLFAN IWKKPLLQPF LRLTYQQAFE LYGSDKPDLR NPLKITDFTT 310 320 330 340 350 360 FFDTNTYSQN IFAGKIKGFK VSKTAFLTRR KLDEYQLFFS KHFNLKLFSF VKKNDKIIGG 370 380 390 400 410 420 ISQFIQDDSF LKNEEICFVV SGKKDIIHKA LGIFRTKLAL DLSLVDTTQE ALLWIVDFPL 430 440 450 460 470 480 FETIQEDLSP PNRLYSLHHP FTAPRDATIL KSNPQKALAN AYDLVWNGYE VGGGSLRINN 490 500 510 520 530 540 HQTQELIFSL LGFLQEEVQN RFGFLIEALK YGTPPHGGIA LGLDRLVMLF TKTNNIKDVI 550 560 570 AFPKTQSAKD LMLEAPSAVN QEQLNTLKLQ LKCNFN

References

[1]

"Living with genome instability: the adaptation of phytoplasmas to diverse environments of their insect and plant hosts." Bai X., Zhang J., Ewing A., Miller S.A., Jancso Radek A., Shevchenko D.V., Tsukerman K., Walunas T., Lapidus A., Campbell J.W., Hogenhout S.A. J. Bacteriol. 188:3682-3696(2006) [PubMed: 16672622] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000061 Genomic DNA. Translation: ABC65710.1.
RefSeq	YP_456789.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3866086.
GenomeReviews	Gene locus AYWB_593 in contig CP000061_GR.
KEGG	ayw:AYWB_593.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2NIN3.
OMA	Q2NIN3. VDRRRDH.
Family and domain databases
HAMAP	MF_00044. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-reg. IPR002312. Asp-tRNA-synth_IIb. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR018153. Asp-tRNA-synth_IIb_C_bac/mt. IPR012340. NA-bd_OB-fold. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00459. aspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_AYWBP

Accession

Primary (citable) accession number: Q2NIN3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	February 7, 2006
Last modified:	June 16, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)