Reviewed, UniProtKB/Swiss-Prot Q2NIN2 (SYH_AYWBP)

Names and origin

Protein names

Recommended name:
    Histidyl-tRNA synthetase
    EC=6.1.1.21
Alternative name(s):
    Histidine--tRNA ligase
      Short name=HisRS

Gene names

Name:	hisS
Ordered Locus Names:	AYWB_594

Organism

Aster yellows witches'-broom phytoplasma (strain AYWB) [Complete proteome] [HAMAP]

Taxonomic identifier

322098 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Acholeplasmatales › Acholeplasmataceae › Candidatus Phytoplasma › Candidatus Phytoplasma asteris

Protein attributes

Sequence length	427 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His). HAMAP MF_00127
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	histidyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP histidine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 427	427	Histidyl-tRNA synthetase HAMAP MF_00127		PRO_1000016311

Sequences

	Sequence		Length	Mass (Da)
	Q2NIN2-1 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 8A750EA3CDD3AD3D	FASTA	427	49,715
	10 20 30 40 50 60 MFSKIKGTHD LMLDKMVCWQ KVENHIRTLF AKYHLQEIRT PIIEYRGVFD RAAQHSEMVS 70 80 90 100 110 120 KETYTFTDKK GRFITLRPEG TAGVIRSYVE NKLDKTSQLH KFFYYGPFFR YERPQKGRYR 130 140 150 160 170 180 QFHQVGVEIL GQSSPFLDVE VIFLAYKTLK SLGICDITVK INSLGCKTTY NNYLQVFKNY 190 200 210 220 230 240 LQTHYQQLCP LCQERFEKNI LRIWDCKNCN NEPFLKQAPR IFDHLVEDAK VRFLQVLEGL 250 260 270 280 290 300 KQMNVNFELC HDLVRGLDYY TNSVFEIVYN NEQGHQAVLG GGGCYDNLVT LFRGSPSPGI 310 320 330 340 350 360 GFALGMERLM SILATRSFCN KNILPSLDAF ILVSEPQFFY QGLELATTLR HQGFSADLNY 370 380 390 400 410 420 KFLSFSKSLK QALKKQPLYL LILGPKEFAN NQITIKNTYT QQQTTILQKD VVSYLQNNKE LNYIHEN

References

[1]

"Living with genome instability: the adaptation of phytoplasmas to diverse environments of their insect and plant hosts." Bai X., Zhang J., Ewing A., Miller S.A., Jancso Radek A., Shevchenko D.V., Tsukerman K., Walunas T., Lapidus A., Campbell J.W., Hogenhout S.A. J. Bacteriol. 188:3682-3696(2006) [PubMed: 16672622] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000061 Genomic DNA. Translation: ABC65711.1.
RefSeq	YP_456790.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3866087.
GenomeReviews	Gene locus AYWB_594 in contig CP000061_GR.
KEGG	ayw:AYWB_594.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2NIN2.
OMA	Q2NIN2. VFEWVTT.
Family and domain databases
HAMAP	MF_00127. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004154. Anticodon_bd. IPR015807. His-tRNA-synth_IIa_sub. IPR004516. His-tRNA_synth_IIA. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
PANTHER	PTHR11476. His-tRNA_synth. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PIRSF	PIRSF001549. His-tRNA_synth. 1 hit.
TIGRFAMs	TIGR00442. hisS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYH_AYWBP

Accession

Primary (citable) accession number: Q2NIN2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 7, 2006
Last modified:	June 16, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)