Q2NIM1 (KITH_AYWBP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidine kinase
EC=2.7.1.21

Gene names

Name:	tdk
Ordered Locus Names:	AYWB_605

Organism

Aster yellows witches'-broom phytoplasma (strain AYWB) [Complete proteome] [HAMAP]

Taxonomic identifier

322098 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Acholeplasmatales › Acholeplasmataceae › Candidatus Phytoplasma › Candidatus Phytoplasma asteris › Aster yellows witches'-broom phytoplasma

Protein attributes

Sequence length	209 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + thymidine = ADP + thymidine 5'-phosphate. HAMAP-Rule MF_00124
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00124.
Sequence similarities	Belongs to the thymidine kinase family.
Sequence caution	The sequence ABC65722.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	DNA synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP thymidine kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 209	209	Thymidine kinase HAMAP-Rule MF_00124		PRO_0000242787
Regions
	Nucleotide binding	16 – 23	8	ATP By similarity
	Nucleotide binding	90 – 93	4	ATP By similarity
Sites
	Active site	91	1	Proton acceptor Potential

Sequences

	Sequence		Length	Mass (Da)
	Q2NIM1 [UniParc]. Last modified June 27, 2006. Version 2. Checksum: B82A9EA00E668308	FASTA	209	23,466
	10 20 30 40 50 60 MTQKEQGQGF IEVICGPMFA GKTESLIQRR NKALKLKKKI LSFKPRIDDR YSVKEEIVSH 70 80 90 100 110 120 NRNNIPAILI DKSKDILTFI TPEINVVIID ESQFLDNDII AIVDYLANCN IEVIISGLEL 130 140 150 160 170 180 DFCGKPFGPM PYLLAIADTV TKLTSICAIS GGKANRTQRL IEGKPAQSNE PVVLVGGKEY 190 200 HEPRCRKHHC LADIDKTKVN WKNFANQSK

References

[1]

"Living with genome instability: the adaptation of phytoplasmas to diverse environments of their insect and plant hosts." Bai X., Zhang J., Ewing A., Miller S.A., Jancso Radek A., Shevchenko D.V., Tsukerman K., Walunas T., Lapidus A., Campbell J.W., Hogenhout S.A. J. Bacteriol. 188:3682-3696(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AYWB.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000061 Genomic DNA. Translation: ABC65722.1. Different initiation.
RefSeq	YP_456801.1. NC_007716.1.
3D structure databases
ProteinModelPortal	Q2NIM1.
ModBase	Search...
Protein-protein interaction databases
STRING	322098.AYWB_605.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABC65722; ABC65722; AYWB_605.
GeneID	3865598.
KEGG	ayw:AYWB_605.
PATRIC	21005276. VBIAstYel136969_0666.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1435.
HOGENOM	HOG000076390.
KO	K00857.
ProtClustDB	PRK04296.
Enzyme and pathway databases
BioCyc	CPHY322098:GJ7E-643-MONOMER.
Family and domain databases
HAMAP	MF_00124. Thymidine_kinase.
InterPro	IPR001267. Thymidine_kinase. IPR020634. Thymidine_kinase_subgr. [Graphical view]
PANTHER	PTHR11441. PTHR11441. 1 hit.
Pfam	PF00265. TK. 1 hit. [Graphical view]
PIRSF	PIRSF035805. TK_cell. 1 hit.
PROSITE	PS00603. TK_CELLULAR_TYPE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KITH_AYWBP

Accession

Primary (citable) accession number: Q2NIM1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	June 27, 2006
Last modified:	May 1, 2013
This is version 41 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program