Reviewed, UniProtKB/Swiss-Prot Q2NIG3 (SYT_AYWBP)

Names and origin

Protein names

Recommended name:
    Threonyl-tRNA synthetase
    EC=6.1.1.3
Alternative name(s):
    Threonine--tRNA ligase
      Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	AYWB_663

Organism

Aster yellows witches'-broom phytoplasma (strain AYWB) [Complete proteome] [HAMAP]

Taxonomic identifier

322098 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Acholeplasmatales › Acholeplasmataceae › Candidatus Phytoplasma › Candidatus Phytoplasma asteris

Protein attributes

Sequence length	641 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00184
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP threonine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 641	641	Threonyl-tRNA synthetase HAMAP MF_00184		PRO_1000020339
Regions
	Region	240 – 538	299	Catalytic HAMAP MF_00184
Sites
	Metal binding	334	1	Zinc; catalytic By similarity
	Metal binding	385	1	Zinc; catalytic By similarity
	Metal binding	515	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2NIG3-1 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: C94278E10EB71C31	FASTA	641	74,950
	10 20 30 40 50 60 MIKISFFDNQ IQEFISGTTP LAIWKEHLSE HFKKPVAALF NNQPIELDFP LKKNGNLEIL 70 80 90 100 110 120 TESDPKSLEV LNHSTAHLMA QAVARLYPDA LFTVGPAIKE GFYYDIDFQK HTISEKDFLT 130 140 150 160 170 180 IEKVMHQISL ENHKITREEI SFEKAKKLFA YNPYKQVLLE KFRDQTISIY RQGEFFDLCR 190 200 210 220 230 240 GVHVIKTNLI KHFKILKISG AYFQGDAKNK TLTRIYGTSF FKKQALADYL QLLEERKERD 250 260 270 280 290 300 HKKINKELDL FMFNKEVGLG LPFWLPKGAT VRRIVERYIV DKELSYDYHH VYTPIMANTE 310 320 330 340 350 360 LYRISGHLDH YASNMFPIMS LENGEKLVLR PMNCPHHMMI YKKNPHSYKE LPLRIAELGM 370 380 390 400 410 420 MHRFEKSGAV SGLQRVREMT LNDAHIFARP DQIKEEIKKI INLILEVYCD FNIKNYELRL 430 440 450 460 470 480 SYRNPNNTEK YFNDDQMWYN AEKTLKETIK ELGLPFKEAI GEAAFYGPKL DVQVFNALGN 490 500 510 520 530 540 EETLSTVQLD FLLPQKFDLT FIGEDNKHHR PVVIHRAVVS TMERFLAHLV EETKGVFPLW 550 560 570 580 590 600 LAPVQVLLIP VSAPLHFEFS QKIKETLQLQ NFRVEINSKD NTLGYKIREA QKLKIPYQVV 610 620 630 640 IGDHEMINNL ITFRKYGSHL QTTIKVDEFV SLLKDKVLQK K

References

[1]

"Living with genome instability: the adaptation of phytoplasmas to diverse environments of their insect and plant hosts." Bai X., Zhang J., Ewing A., Miller S.A., Jancso Radek A., Shevchenko D.V., Tsukerman K., Walunas T., Lapidus A., Campbell J.W., Hogenhout S.A. J. Bacteriol. 188:3682-3696(2006) [PubMed: 16672622] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000061 Genomic DNA. Translation: ABC65780.1.
RefSeq	YP_456859.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3865656.
GenomeReviews	Gene locus AYWB_663 in contig CP000061_GR.
KEGG	ayw:AYWB_663.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2NIG3.
OMA	Q2NIG3. IQLDFML.
Family and domain databases
HAMAP	MF_00184. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004154. Anticodon_bd. IPR012675. b-grasp_ferredoxin-like. IPR002320. Thr-tRNA-synth_IIa. IPR018158. Thr-tRNA-synth_IIa_cons-reg. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
TIGRFAMs	TIGR00418. thrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT_AYWBP

Accession

Primary (citable) accession number: Q2NIG3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 7, 2006
Last modified:	June 16, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)