Reviewed, UniProtKB/Swiss-Prot Q2NIF5 (SYN_AYWBP)

Names and origin

Protein names

Recommended name:
    Asparaginyl-tRNA synthetase
    EC=6.1.1.22
Alternative name(s):
    Asparagine--tRNA ligase
      Short name=AsnRS

Gene names

Name:	asnS
Ordered Locus Names:	AYWB_671

Organism

Aster yellows witches'-broom phytoplasma (strain AYWB) [Complete proteome] [HAMAP]

Taxonomic identifier

322098 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Acholeplasmatales › Acholeplasmataceae › Candidatus Phytoplasma › Candidatus Phytoplasma asteris

Protein attributes

Sequence length	503 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP MF_00534
Subunit structure	Homodimer By similarity. HAMAP MF_00534
Subcellular location	Cytoplasm HAMAP MF_00534.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP asparagine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP aspartate-tRNA ligase activity Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 503	503	Asparaginyl-tRNA synthetase HAMAP MF_00534		PRO_1000051375

Sequences

	Sequence		Length	Mass (Da)
	Q2NIF5-1 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 59190A36EDBD1A90	FASTA	503	58,286
	10 20 30 40 50 60 MKISIKDIFQ KPELFYQKKI LLNGWVRNCR YQKKLIFIDL NDGTFLENLQ IVCKEIKNIE 70 80 90 100 110 120 TADINEFQEN QKFNNNNKSS NNINLEKLKE ILQIGASLQV EGILKATNNL KTPFEISAQN 130 140 150 160 170 180 ISLLGTSDFS YPLQPKKHSK VFLRQISHLR VRTKLFGAVF RIRNTAFFAL HSFFQKKGFF 190 200 210 220 230 240 HINTPIITPN DGEGVGELFQ ITTLNLEVLP QTKNIPNAFK PVNENTSKKG IDYKKDFFGK 250 260 270 280 290 300 KVFLTVTGQL EAEAMALGLN KVYTFGPTFR SEKSNTTRHA AEFWMLEPEM AFCDLSQNLK 310 320 330 340 350 360 VAQEMLQFVI SKCLEQNYQD IEFLDKTEKN GLIEELQNIA EEKEFLTVKY EQALEILQKS 370 380 390 400 410 420 NTKFENPLFY GVDLATEHEK YLTEKHFKKP VFIVDWPKEI KAFYMKNNPD QKTVAAMDLL 430 440 450 460 470 480 FPRVGELIGG SQREENLSVL IEKMNQMKIS QKDLEWYLDL RRFGGCIHSG FGLGFERLLI 490 500 FLTGLDNIRD VIAFPRTYHN LVF

References

[1]

"Living with genome instability: the adaptation of phytoplasmas to diverse environments of their insect and plant hosts." Bai X., Zhang J., Ewing A., Miller S.A., Jancso Radek A., Shevchenko D.V., Tsukerman K., Walunas T., Lapidus A., Campbell J.W., Hogenhout S.A. J. Bacteriol. 188:3682-3696(2006) [PubMed: 16672622] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000061 Genomic DNA. Translation: ABC65788.1.
RefSeq	YP_456867.1.
3D structure databases
HSSP	HSSP built from PDB template 1X56 based on UniProtKB O57980.
SMR	Q2NIF5. Positions 16-501.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2NIF5.
Genome annotation databases
GeneID	3865664.
GenomeReviews	Gene locus AYWB_671 in contig CP000061_GR.
KEGG	ayw:AYWB_671.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0017.
HOGENOM	HBG745843.
OMA	LQKKRHS.
PhylomeDB	Q2NIF5.
Family and domain databases
HAMAP	MF_00534. Asn_tRNA_synth. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-dom. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp-tRNA-synth_IIb. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00457. asnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYN_AYWBP

Accession

Primary (citable) accession number: Q2NIF5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 7, 2006
Last modified:	February 9, 2010
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)