Q2NIA7 (Q2NIA7_METST) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Non-canonical purine NTP pyrophosphatase HAMAP MF_01405
EC=3.6.1.19 HAMAP MF_01405

Alternative name(s):
Non-standard purine NTP pyrophosphatase HAMAP MF_01405
Nucleoside-triphosphate diphosphatase HAMAP MF_01405
Nucleoside-triphosphate pyrophosphatase HAMAP MF_01405

Gene names

Ordered Locus Names:

Msp_0011

Organism

Methanosphaera stadtmanae (strain DSM 3091) [Complete proteome] [HAMAP]

Taxonomic identifier

339860 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanosphaera

Protein attributes

Sequence length	183 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions By similarity. HAMAP MF_01405
Catalytic activity	A nucleoside triphosphate + H2O = a nucleotide + diphosphate. HAMAP MF_01405
Cofactor	Binds 1 divalent metal cation ion per subunit; can use either magnesium or manganese By similarity. HAMAP MF_01405
Subunit structure	Homodimer By similarity. HAMAP MF_01405
Sequence similarities	Belongs to the HAM1 NTPase family. HAMAP MF_01405 RuleBase RU003781

Ontologies

Keywords
Biological process	Nucleotide metabolism HAMAP MF_01405
Ligand	Magnesium HAMAP MF_01405 Manganese HAMAP MF_01405 Metal-binding HAMAP MF_01405 Nucleotide-binding HAMAP MF_01405
Molecular function	Hydrolase HAMAP MF_01405 RuleBase RU003781
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro nucleoside-triphosphate diphosphatase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	7 – 12	6	Substrate binding By similarity HAMAP MF_01405
	Region	64 – 65	2	Substrate binding By similarity HAMAP MF_01405
Sites
	Metal binding	36	1	Magnesium or manganese By similarity HAMAP MF_01405
	Metal binding	64	1	Magnesium or manganese By similarity HAMAP MF_01405
	Binding site	143	1	Substrate By similarity HAMAP MF_01405
	Binding site	163	1	Substrate By similarity HAMAP MF_01405
	Binding site	169	1	Substrate By similarity HAMAP MF_01405

Sequences

	Sequence		Length	Mass (Da)
	Q2NIA7 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: AB21EC3FADA300E4	FASTA	183	20,784
	10 20 30 40 50 60 MRVTFITGNK HKVKEARGIF EKFGIEVDHE NPGYPEVQGS IEEVAAFGAK YVADLLQKPV 70 80 90 100 110 120 IVDDTGLFIR ALNDFPGTYS SYVQDTITNK GILKLMKDEP DRYAEFRSCI GYCAPNCEPK 130 140 150 160 170 180 TFLGVVSGEI LSEEKGNNGF AYDPLFYVEK YDKTYGELTT DEKNECSHRR LSMEKFANWY SNL

References

[1]

"The genome sequence of Methanosphaera stadtmanae reveals why this human intestinal archaeon is restricted to methanol and H2 for methane formation and ATP synthesis." Fricke W.F., Seedorf H., Henne A., Kruer M., Liesegang H., Hedderich R., Gottschalk G., Thauer R.K. J. Bacteriol. 188:642-658(2006) [PubMed: 16385054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000102 Genomic DNA. Translation: ABC56431.1.
RefSeq	YP_447074.1. NC_007681.1.
3D structure databases
ProteinModelPortal	Q2NIA7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2NIA7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3855637.
GenomeReviews	Gene locus Msp_0011 in contig CP000102_GR.
KEGG	mst:Msp_0011.
NMPDR	fig|339860.6.peg.12.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG10472.
HOGENOM	HBG697237.
OMA	VEDSGFF.
PhylomeDB	Q2NIA7.
ProtClustDB	CLSK798170.
Family and domain databases
HAMAP	MF_01405. Non_canon_purine_NTPase. [Tree]
InterPro	IPR002637. Ham1p-like. IPR020922. Nucleoside-triphosphatase. [Graphical view]
KO	K02428.
PANTHER	PTHR11067. Ham1p_like. 1 hit.
Pfam	PF01725. Ham1p_like. 1 hit. [Graphical view]
TIGRFAMs	TIGR00042. TIGR00042. 1 hit.
ProtoNet	Search...

Entry information

Entry name

Q2NIA7_METST

Accession

Primary (citable) accession number: Q2NIA7

Entry history

Integrated into UniProtKB/TrEMBL:	February 7, 2006
Last sequence update:	February 7, 2006
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)