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Q2NIA4

- KAE1B_METST

UniProt

Q2NIA4 - KAE1B_METST

Protein

Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein

Gene

Msp_0013

Organism
Methanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (07 Feb 2006)
      Previous versions | rss
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    Functioni

    Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction. The Bud32 domain probably displays kinase activity that regulates Kae1 function.UniRule annotation

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.UniRule annotation
    L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

    Cofactori

    Binds 1 Fe2+ ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi108 – 1081IronUniRule annotation
    Metal bindingi112 – 1121IronUniRule annotation
    Metal bindingi129 – 1291IronUniRule annotation
    Binding sitei161 – 1611Threonylcarbamoyl-AMPUniRule annotation
    Binding sitei174 – 1741Threonylcarbamoyl-AMP; via amide nitrogenUniRule annotation
    Binding sitei178 – 1781Threonylcarbamoyl-AMPUniRule annotation
    Binding sitei258 – 2581Threonylcarbamoyl-AMPUniRule annotation
    Metal bindingi286 – 2861IronUniRule annotation
    Binding sitei361 – 3611ATPUniRule annotation
    Active sitei455 – 4551Proton acceptor; for kinase activityUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi340 – 3489ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. iron ion binding Source: UniProtKB-HAMAP
    3. metalloendopeptidase activity Source: InterPro
    4. protein serine/threonine/tyrosine kinase activity Source: UniProtKB-HAMAP
    5. protein serine/threonine kinase activity Source: UniProtKB-KW
    6. protein tyrosine kinase activity Source: InterPro
    7. transferase activity, transferring acyl groups other than amino-acyl groups Source: UniProtKB-HAMAP
    8. zinc ion binding Source: InterPro

    GO - Biological processi

    1. threonylcarbamoyladenosine biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Acyltransferase, Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    ATP-binding, Iron, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMSTA339860:GJEZ-13-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis proteinUniRule annotation
    Including the following 2 domains:
    tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.6.99.4UniRule annotation)
    Alternative name(s):
    N6-L-threonylcarbamoyladenine synthase
    Short name:
    t(6)A synthase
    t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1UniRule annotation
    tRNA threonylcarbamoyladenosine biosynthesis protein Kae1UniRule annotation
    Serine/threonine-protein kinase Bud32UniRule annotation (EC:2.7.11.1UniRule annotation)
    Gene namesi
    Ordered Locus Names:Msp_0013
    OrganismiMethanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3)
    Taxonomic identifieri339860 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanosphaera
    ProteomesiUP000001931: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 534534Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis proteinPRO_0000303657Add
    BLAST

    Interactioni

    Subunit structurei

    Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes.UniRule annotation

    Protein-protein interaction databases

    STRINGi339860.Msp_0013.

    Structurei

    3D structure databases

    ProteinModelPortaliQ2NIA4.
    SMRiQ2NIA4. Positions 1-325.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini335 – 534200Protein kinaseUniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 324324Kae1Add
    BLAST
    Regioni129 – 1335Threonylcarbamoyl-AMP bindingUniRule annotation

    Sequence similaritiesi

    In the N-terminal section; belongs to the KAE1 / TsaD family.UniRule annotation
    In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily.UniRule annotation
    Contains 1 protein kinase domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0533.
    HOGENOMiHOG000109569.
    KOiK15904.
    OMAiRDNAGMI.

    Family and domain databases

    HAMAPiMF_01447. Kae1_Bud32_arch.
    InterProiIPR022495. Bud32.
    IPR000905. Gcp-like_dom.
    IPR022449. Kae1.
    IPR017861. KAE1/YgjD.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR018934. RIO-like_kinase.
    IPR009220. tRNA_threonyl_synthase/kinase.
    IPR008266. Tyr_kinase_AS.
    [Graphical view]
    PfamiPF00814. Peptidase_M22. 1 hit.
    PF01163. RIO1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF036401. Gcp_STYKS. 1 hit.
    PRINTSiPR00789. OSIALOPTASE.
    SUPFAMiSSF56112. SSF56112. 1 hit.
    TIGRFAMsiTIGR03724. arch_bud32. 1 hit.
    TIGR03722. arch_KAE1. 1 hit.
    TIGR00329. gcp_kae1. 1 hit.
    PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q2NIA4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MICLGIEGTA EKCGIGIVDS DGNILATCGC QLYPEVGGIH PREAANFHAE    50
    HIVPLIREAL EESNLSINDI DLVSFAKGPG LGPALRTVAT AARSLSQNIG 100
    VPLIGVNHCI GHVEIGKLTT GAKDPLTLYT SGGNTQIISY ESGRYRIIGE 150
    TLDIAIGNCL DQFSRDIGLG HPGGPIVEKH AENTNKTIEL PYVVKGMDLS 200
    FSGILTSAIN KYKQGVDLDV ICNSFQQTCF AMLCEVTERA ISYTGKNEVL 250
    LCGGVAANSK LRQMLQVMCE DHYVDFYMPP MKYCGDNGSM IARVGLLSYD 300
    ENKCGIENSY INPKYRTDQV EVTWIKDNFQ HNINLPDNIK EKGAEADIIE 350
    GIWDEREVII KHRVKKKYRV DKLDEKLRLE RTKEEAKLLS DCKKYGVTTP 400
    YIYSIDLNRK DIILEKIDAP KLHEIINTDK DIKKIFTNIG EMVFKMHEGE 450
    IIHGDLTTAN ILIKDDKPIF IDFGLGKYSN LLEDKGTDLL VFKKSLNTII 500
    PEKSEELFNY FLNGYDNEDV IKKIDEIEKR GRYL 534
    Length:534
    Mass (Da):59,651
    Last modified:February 7, 2006 - v1
    Checksum:iDCFD1FFE623129B7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000102 Genomic DNA. Translation: ABC56433.1.
    RefSeqiWP_011405632.1. NC_007681.1.
    YP_447076.1. NC_007681.1.

    Genome annotation databases

    EnsemblBacteriaiABC56433; ABC56433; Msp_0013.
    GeneIDi3855639.
    KEGGimst:Msp_0013.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000102 Genomic DNA. Translation: ABC56433.1 .
    RefSeqi WP_011405632.1. NC_007681.1.
    YP_447076.1. NC_007681.1.

    3D structure databases

    ProteinModelPortali Q2NIA4.
    SMRi Q2NIA4. Positions 1-325.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 339860.Msp_0013.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABC56433 ; ABC56433 ; Msp_0013 .
    GeneIDi 3855639.
    KEGGi mst:Msp_0013.

    Phylogenomic databases

    eggNOGi COG0533.
    HOGENOMi HOG000109569.
    KOi K15904.
    OMAi RDNAGMI.

    Enzyme and pathway databases

    BioCyci MSTA339860:GJEZ-13-MONOMER.

    Family and domain databases

    HAMAPi MF_01447. Kae1_Bud32_arch.
    InterProi IPR022495. Bud32.
    IPR000905. Gcp-like_dom.
    IPR022449. Kae1.
    IPR017861. KAE1/YgjD.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR018934. RIO-like_kinase.
    IPR009220. tRNA_threonyl_synthase/kinase.
    IPR008266. Tyr_kinase_AS.
    [Graphical view ]
    Pfami PF00814. Peptidase_M22. 1 hit.
    PF01163. RIO1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF036401. Gcp_STYKS. 1 hit.
    PRINTSi PR00789. OSIALOPTASE.
    SUPFAMi SSF56112. SSF56112. 1 hit.
    TIGRFAMsi TIGR03724. arch_bud32. 1 hit.
    TIGR03722. arch_KAE1. 1 hit.
    TIGR00329. gcp_kae1. 1 hit.
    PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Methanosphaera stadtmanae reveals why this human intestinal archaeon is restricted to methanol and H2 for methane formation and ATP synthesis."
      Fricke W.F., Seedorf H., Henne A., Kruer M., Liesegang H., Hedderich R., Gottschalk G., Thauer R.K.
      J. Bacteriol. 188:642-658(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3.

    Entry informationi

    Entry nameiKAE1B_METST
    AccessioniPrimary (citable) accession number: Q2NIA4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 11, 2007
    Last sequence update: February 7, 2006
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3