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Q2NIA4

- KAE1B_METST

UniProt

Q2NIA4 - KAE1B_METST

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Protein

Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein

Gene

Msp_0013

Organism
Methanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction. The Bud32 domain probably displays kinase activity that regulates Kae1 function.UniRule annotation

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.UniRule annotation
L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

Binds 1 Fe2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi108 – 1081IronUniRule annotation
Metal bindingi112 – 1121IronUniRule annotation
Metal bindingi129 – 1291IronUniRule annotation
Binding sitei161 – 1611Threonylcarbamoyl-AMPUniRule annotation
Binding sitei174 – 1741Threonylcarbamoyl-AMP; via amide nitrogenUniRule annotation
Binding sitei178 – 1781Threonylcarbamoyl-AMPUniRule annotation
Binding sitei258 – 2581Threonylcarbamoyl-AMPUniRule annotation
Metal bindingi286 – 2861IronUniRule annotation
Binding sitei361 – 3611ATPUniRule annotation
Active sitei455 – 4551Proton acceptor; for kinase activityUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi340 – 3489ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. iron ion binding Source: UniProtKB-HAMAP
  3. metalloendopeptidase activity Source: InterPro
  4. protein serine/threonine/tyrosine kinase activity Source: UniProtKB-HAMAP
  5. protein serine/threonine kinase activity Source: UniProtKB-KW
  6. transferase activity, transferring acyl groups other than amino-acyl groups Source: UniProtKB-HAMAP
  7. zinc ion binding Source: InterPro

GO - Biological processi

  1. threonylcarbamoyladenosine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Iron, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMSTA339860:GJEZ-13-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis proteinUniRule annotation
Including the following 2 domains:
tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.6.99.4UniRule annotation)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthase
Short name:
t(6)A synthase
t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1UniRule annotation
tRNA threonylcarbamoyladenosine biosynthesis protein Kae1UniRule annotation
Serine/threonine-protein kinase Bud32UniRule annotation (EC:2.7.11.1UniRule annotation)
Gene namesi
Ordered Locus Names:Msp_0013
OrganismiMethanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3)
Taxonomic identifieri339860 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanosphaera
ProteomesiUP000001931: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 534534Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis proteinPRO_0000303657Add
BLAST

Interactioni

Subunit structurei

Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes.UniRule annotation

Protein-protein interaction databases

STRINGi339860.Msp_0013.

Structurei

3D structure databases

ProteinModelPortaliQ2NIA4.
SMRiQ2NIA4. Positions 1-325.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini335 – 534200Protein kinaseUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 324324Kae1Add
BLAST
Regioni129 – 1335Threonylcarbamoyl-AMP bindingUniRule annotation

Sequence similaritiesi

In the N-terminal section; belongs to the KAE1 / TsaD family.UniRule annotation
In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily.UniRule annotation
Contains 1 protein kinase domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0533.
HOGENOMiHOG000109569.
KOiK15904.
OMAiRDNAGMI.

Family and domain databases

HAMAPiMF_01447. Kae1_Bud32_arch.
InterProiIPR022495. Bud32.
IPR000905. Gcp-like_dom.
IPR022449. Kae1.
IPR017861. KAE1/YgjD.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR018934. RIO-like_kinase.
IPR009220. tRNA_threonyl_synthase/kinase.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
PF01163. RIO1. 1 hit.
[Graphical view]
PIRSFiPIRSF036401. Gcp_STYKS. 1 hit.
PRINTSiPR00789. OSIALOPTASE.
SUPFAMiSSF56112. SSF56112. 1 hit.
TIGRFAMsiTIGR03724. arch_bud32. 1 hit.
TIGR03722. arch_KAE1. 1 hit.
TIGR00329. gcp_kae1. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2NIA4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MICLGIEGTA EKCGIGIVDS DGNILATCGC QLYPEVGGIH PREAANFHAE
60 70 80 90 100
HIVPLIREAL EESNLSINDI DLVSFAKGPG LGPALRTVAT AARSLSQNIG
110 120 130 140 150
VPLIGVNHCI GHVEIGKLTT GAKDPLTLYT SGGNTQIISY ESGRYRIIGE
160 170 180 190 200
TLDIAIGNCL DQFSRDIGLG HPGGPIVEKH AENTNKTIEL PYVVKGMDLS
210 220 230 240 250
FSGILTSAIN KYKQGVDLDV ICNSFQQTCF AMLCEVTERA ISYTGKNEVL
260 270 280 290 300
LCGGVAANSK LRQMLQVMCE DHYVDFYMPP MKYCGDNGSM IARVGLLSYD
310 320 330 340 350
ENKCGIENSY INPKYRTDQV EVTWIKDNFQ HNINLPDNIK EKGAEADIIE
360 370 380 390 400
GIWDEREVII KHRVKKKYRV DKLDEKLRLE RTKEEAKLLS DCKKYGVTTP
410 420 430 440 450
YIYSIDLNRK DIILEKIDAP KLHEIINTDK DIKKIFTNIG EMVFKMHEGE
460 470 480 490 500
IIHGDLTTAN ILIKDDKPIF IDFGLGKYSN LLEDKGTDLL VFKKSLNTII
510 520 530
PEKSEELFNY FLNGYDNEDV IKKIDEIEKR GRYL
Length:534
Mass (Da):59,651
Last modified:February 7, 2006 - v1
Checksum:iDCFD1FFE623129B7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000102 Genomic DNA. Translation: ABC56433.1.
RefSeqiWP_011405632.1. NC_007681.1.
YP_447076.1. NC_007681.1.

Genome annotation databases

EnsemblBacteriaiABC56433; ABC56433; Msp_0013.
GeneIDi3855639.
KEGGimst:Msp_0013.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000102 Genomic DNA. Translation: ABC56433.1 .
RefSeqi WP_011405632.1. NC_007681.1.
YP_447076.1. NC_007681.1.

3D structure databases

ProteinModelPortali Q2NIA4.
SMRi Q2NIA4. Positions 1-325.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 339860.Msp_0013.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABC56433 ; ABC56433 ; Msp_0013 .
GeneIDi 3855639.
KEGGi mst:Msp_0013.

Phylogenomic databases

eggNOGi COG0533.
HOGENOMi HOG000109569.
KOi K15904.
OMAi RDNAGMI.

Enzyme and pathway databases

BioCyci MSTA339860:GJEZ-13-MONOMER.

Family and domain databases

HAMAPi MF_01447. Kae1_Bud32_arch.
InterProi IPR022495. Bud32.
IPR000905. Gcp-like_dom.
IPR022449. Kae1.
IPR017861. KAE1/YgjD.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR018934. RIO-like_kinase.
IPR009220. tRNA_threonyl_synthase/kinase.
IPR008266. Tyr_kinase_AS.
[Graphical view ]
Pfami PF00814. Peptidase_M22. 1 hit.
PF01163. RIO1. 1 hit.
[Graphical view ]
PIRSFi PIRSF036401. Gcp_STYKS. 1 hit.
PRINTSi PR00789. OSIALOPTASE.
SUPFAMi SSF56112. SSF56112. 1 hit.
TIGRFAMsi TIGR03724. arch_bud32. 1 hit.
TIGR03722. arch_KAE1. 1 hit.
TIGR00329. gcp_kae1. 1 hit.
PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of Methanosphaera stadtmanae reveals why this human intestinal archaeon is restricted to methanol and H2 for methane formation and ATP synthesis."
    Fricke W.F., Seedorf H., Henne A., Kruer M., Liesegang H., Hedderich R., Gottschalk G., Thauer R.K.
    J. Bacteriol. 188:642-658(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3.

Entry informationi

Entry nameiKAE1B_METST
AccessioniPrimary (citable) accession number: Q2NIA4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: February 7, 2006
Last modified: October 29, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3