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Q2NIA4 (KAE1B_METST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein

Including the following 2 domains:

  1. tRNA N6-adenosine threonylcarbamoyltransferase
    EC=2.6.99.4
    Alternative name(s):
    N6-L-threonylcarbamoyladenine synthase
    Short name=t(6)A synthase
    t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1
    tRNA threonylcarbamoyladenosine biosynthesis protein Kae1
  2. Serine/threonine-protein kinase Bud32
    EC=2.7.11.1
Gene names
Ordered Locus Names:Msp_0013
OrganismMethanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3) [Complete proteome] [HAMAP]
Taxonomic identifier339860 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanosphaera

Protein attributes

Sequence length534 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction. The Bud32 domain probably displays kinase activity that regulates Kae1 function By similarity. HAMAP-Rule MF_01447

Catalytic activity

ATP + a protein = ADP + a phosphoprotein. HAMAP-Rule MF_01447

L-threonylcarbamoyladenylate + adenine37 in tRNA = AMP + N(6)-L-threonylcarbamoyladenine37 in tRNA. HAMAP-Rule MF_01447

Cofactor

Binds 1 Fe2+ ion per subunit By similarity. HAMAP-Rule MF_01447

Subunit structure

Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes By similarity.

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01447.

Sequence similarities

In the N-terminal section; belongs to the KAE1 / TsaD family.

In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 534534Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein HAMAP-Rule MF_01447
PRO_0000303657

Regions

Domain335 – 534200Protein kinase
Nucleotide binding340 – 3489ATP By similarity
Region1 – 324324Kae1 HAMAP-Rule MF_01447
Region129 – 1335Threonylcarbamoyl-AMP binding By similarity

Sites

Active site4551Proton acceptor; for kinase activity By similarity
Metal binding1081Iron By similarity
Metal binding1121Iron By similarity
Metal binding1291Iron By similarity
Metal binding2861Iron By similarity
Binding site1611Threonylcarbamoyl-AMP By similarity
Binding site1741Threonylcarbamoyl-AMP; via amide nitrogen By similarity
Binding site1781Threonylcarbamoyl-AMP By similarity
Binding site2581Threonylcarbamoyl-AMP By similarity
Binding site3611ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2NIA4 [UniParc].

Last modified February 7, 2006. Version 1.
Checksum: DCFD1FFE623129B7

FASTA53459,651
        10         20         30         40         50         60 
MICLGIEGTA EKCGIGIVDS DGNILATCGC QLYPEVGGIH PREAANFHAE HIVPLIREAL 

        70         80         90        100        110        120 
EESNLSINDI DLVSFAKGPG LGPALRTVAT AARSLSQNIG VPLIGVNHCI GHVEIGKLTT 

       130        140        150        160        170        180 
GAKDPLTLYT SGGNTQIISY ESGRYRIIGE TLDIAIGNCL DQFSRDIGLG HPGGPIVEKH 

       190        200        210        220        230        240 
AENTNKTIEL PYVVKGMDLS FSGILTSAIN KYKQGVDLDV ICNSFQQTCF AMLCEVTERA 

       250        260        270        280        290        300 
ISYTGKNEVL LCGGVAANSK LRQMLQVMCE DHYVDFYMPP MKYCGDNGSM IARVGLLSYD 

       310        320        330        340        350        360 
ENKCGIENSY INPKYRTDQV EVTWIKDNFQ HNINLPDNIK EKGAEADIIE GIWDEREVII 

       370        380        390        400        410        420 
KHRVKKKYRV DKLDEKLRLE RTKEEAKLLS DCKKYGVTTP YIYSIDLNRK DIILEKIDAP 

       430        440        450        460        470        480 
KLHEIINTDK DIKKIFTNIG EMVFKMHEGE IIHGDLTTAN ILIKDDKPIF IDFGLGKYSN 

       490        500        510        520        530 
LLEDKGTDLL VFKKSLNTII PEKSEELFNY FLNGYDNEDV IKKIDEIEKR GRYL 

« Hide

References

[1]"The genome sequence of Methanosphaera stadtmanae reveals why this human intestinal archaeon is restricted to methanol and H2 for methane formation and ATP synthesis."
Fricke W.F., Seedorf H., Henne A., Kruer M., Liesegang H., Hedderich R., Gottschalk G., Thauer R.K.
J. Bacteriol. 188:642-658(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000102 Genomic DNA. Translation: ABC56433.1.
RefSeqYP_447076.1. NC_007681.1.

3D structure databases

ProteinModelPortalQ2NIA4.
SMRQ2NIA4. Positions 1-325.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING339860.Msp_0013.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABC56433; ABC56433; Msp_0013.
GeneID3855639.
KEGGmst:Msp_0013.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0533.
HOGENOMHOG000109569.
KOK15904.
OMARDNAGMI.

Enzyme and pathway databases

BioCycMSTA339860:GJEZ-13-MONOMER.

Family and domain databases

HAMAPMF_01447. Kae1_Bud32_arch.
InterProIPR022495. Bud32.
IPR000905. Gcp-like_dom.
IPR022449. Kae1.
IPR017861. KAE1/YgjD.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR018934. RIO-like_kinase.
IPR009220. tRNA_threonyl_synthase/kinase.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamPF00814. Peptidase_M22. 1 hit.
PF01163. RIO1. 1 hit.
[Graphical view]
PIRSFPIRSF036401. Gcp_STYKS. 1 hit.
PRINTSPR00789. OSIALOPTASE.
SUPFAMSSF56112. SSF56112. 1 hit.
TIGRFAMsTIGR03724. arch_bud32. 1 hit.
TIGR03722. arch_KAE1. 1 hit.
TIGR00329. gcp_kae1. 1 hit.
PROSITEPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKAE1B_METST
AccessionPrimary (citable) accession number: Q2NIA4
Entry history
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: February 7, 2006
Last modified: June 11, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families