Q2NI95 (ILVC_METST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ketol-acid reductoisomerase
EC=1.1.1.86

Alternative name(s):
Acetohydroxy-acid isomeroreductase
Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	Msp_0022

Organism

Methanosphaera stadtmanae (strain DSM 3091) [Complete proteome] [HAMAP]

Taxonomic identifier

339860 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanosphaera

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	coenzyme binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Ketol-acid reductoisomerase HAMAP MF_00435		PRO_0000252802
Sites
	Active site	107	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q2NI95 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 867B3D698C860A25	FASTA	330	36,723
	10 20 30 40 50 60 MKVLYDDDVN TNVLENKTIA VIGYGSQGRG QSLNMHDSGL NVIVGLREGG KSWKKAQEDG 70 80 90 100 110 120 LTVKTIEDAS KEADIIHILI PDEIQKTVYE NQIQQYVEPG NTISFSHGYN IHFNRIRVPE 130 140 150 160 170 180 DVNVTMIAPK APGSKVRQTY QEGFGTPGLI AIEQDATGDA YDIAIEMAKA VGLTRAGVIE 190 200 210 220 230 240 TTYREETETD LFGEQAVLCG GLTGLIQAGF ETLVEAGYKP EMAYFETCHE MKLIVDLIYE 250 260 270 280 290 300 KGFGNMWHDV SNTAEFGGLT RRDRVITEES KENMKKILRE IQDGTFATEF AVDADTSYPK 310 320 330 LLTQRRLEGE KQIEKVGKNL RIACGLQKEE

References

[1]

"The genome sequence of Methanosphaera stadtmanae reveals why this human intestinal archaeon is restricted to methanol and H2 for methane formation and ATP synthesis." Fricke W.F., Seedorf H., Henne A., Kruer M., Liesegang H., Hedderich R., Gottschalk G., Thauer R.K. J. Bacteriol. 188:642-658(2006) [PubMed: 16385054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 3091.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000102 Genomic DNA. Translation: ABC56441.1.
RefSeq	YP_447084.1. NC_007681.1.
3D structure databases
ProteinModelPortal	Q2NI95.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2NI95.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3855648.
GenomeReviews	Gene locus Msp_0022 in contig CP000102_GR.
KEGG	mst:Msp_0022.
NMPDR	fig|339860.6.peg.22.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04335.
HOGENOM	HBG286525.
OMA	EYANGRG.
PhylomeDB	Q2NI95.
ProtClustDB	PRK05479.
Enzyme and pathway databases
BioCyc	MSTA339860:MSP_0022-MONOMER.
Family and domain databases
HAMAP	MF_00435. IlvC. [Tree]
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013328. DH_multihelical. IPR013116. IlvN. [Graphical view]
Gene3D	G3DSA:1.10.1040.10. Opine_DH. 1 hit.
KO	K00053.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
TIGRFAMs	TIGR00465. IlvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_METST

Accession

Primary (citable) accession number: Q2NI95

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 17, 2006
Last sequence update:	February 7, 2006
Last modified:	November 16, 2011
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program