Reviewed, UniProtKB/Swiss-Prot Q2NI86 (PUR2_METST)

Names and origin

Protein names

Recommended name:
    Phosphoribosylamine--glycine ligase
    EC=6.3.4.13
Alternative name(s):
    GARS
    Glycinamide ribonucleotide synthetase
    Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	Msp_0030

Organism

Methanosphaera stadtmanae (strain DSM 3091) [Complete proteome] [HAMAP]

Taxonomic identifier

339860 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanosphaera

Protein attributes

Sequence length	438 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP MF_00138
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP MF_00138
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 438	438	Phosphoribosylamine--glycine ligase HAMAP MF_00138		PRO_1000018829
Regions
	Domain	107 – 319	213	ATP-grasp
	Nucleotide binding	134 – 197	64	ATP By similarity
Sites
	Metal binding	277	1	Magnesium or manganese 1 By similarity
	Metal binding	289	1	Magnesium or manganese 1 By similarity
	Metal binding	289	1	Magnesium or manganese 2 By similarity
	Metal binding	291	1	Magnesium or manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2NI86-1 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: C59F9094D2DA0186	FASTA	438	48,794
	10 20 30 40 50 60 MKILMVGSGA REHAIAKALN KTADVYTYMG RKNPGLARIS KNYTVNDESN FNEIIKFAQE 70 80 90 100 110 120 NNVELAFIGP EAPLEMGIVD ELEKEGIHSV GPTKSAAQIE TNKAFMRKLF EDYDIPGSIK 130 140 150 160 170 180 YGTFYDLDEA YEFIDNFDEP VVVKPIGLTG GKGVKIVGDQ LADNDEAKEY VKEIFHQKMG 190 200 210 220 230 240 GFEGVVIEEL LLGEEYTIQA FVDGTHLIPM PAAQDHPHAF VGNKGPITGG MGSYSDKNHL 250 260 270 280 290 300 LPFLTQEDYD KSVEIMEKTI KAIAKEQEPY KGILYGQFML CKDGPKVIEY NARFGDPESM 310 320 330 340 350 360 NVLSVIDDDL AKISKQIVDG TLDSVNFLNK SSVCKYVVPD KYPNTHVADT VVDVDEDKIN 370 380 390 400 410 420 ELGAQVFYAA VYQDMDDSIK LTSSRALGVL AVKDSIKEAE KICEEAIKYV KGEVYHRNDV 430 ATEELLNEKI KHMEEVRL

References

[1]

"The genome sequence of Methanosphaera stadtmanae reveals why this human intestinal archaeon is restricted to methanol and H2 for methane formation and ATP synthesis." Fricke W.F., Seedorf H., Henne A., Kruer M., Liesegang H., Hedderich R., Gottschalk G., Thauer R.K. J. Bacteriol. 188:642-658(2006) [PubMed: 16385054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000102 Genomic DNA. Translation: ABC56449.1.
RefSeq	YP_447092.1.
3D structure databases
SMR	Q2NI86. Positions 1-425.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2NI86.
Genome annotation databases
GeneID	3855038.
GenomeReviews	Gene locus Msp_0030 in contig CP000102_GR.
KEGG	mst:Msp_0030.
NMPDR	fig|339860.6.peg.29.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04186.
HOGENOM	HBG404060.
OMA	FSLMAFV.
Enzyme and pathway databases
BioCyc	MSTA339860:MSP_0030-MONOMER.
Family and domain databases
HAMAP	MF_00138. GARS. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR016185. PreATP-grasp-like. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N-dom. IPR011054. Rudment_hybrid_motif. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00877. purD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_METST

Accession

Primary (citable) accession number: Q2NI86

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 7, 2006
Last modified:	February 9, 2010
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)