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Q2NI86 (PUR2_METST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:Msp_0030
OrganismMethanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3) [Complete proteome] [HAMAP]
Taxonomic identifier339860 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanosphaera

Protein attributes

Sequence length438 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 438438Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_1000018829

Regions

Domain107 – 319213ATP-grasp
Nucleotide binding134 – 19764ATP By similarity

Sites

Metal binding2771Magnesium or manganese 1 By similarity
Metal binding2891Magnesium or manganese 1 By similarity
Metal binding2891Magnesium or manganese 2 By similarity
Metal binding2911Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2NI86 [UniParc].

Last modified February 7, 2006. Version 1.
Checksum: C59F9094D2DA0186

FASTA43848,794
        10         20         30         40         50         60 
MKILMVGSGA REHAIAKALN KTADVYTYMG RKNPGLARIS KNYTVNDESN FNEIIKFAQE 

        70         80         90        100        110        120 
NNVELAFIGP EAPLEMGIVD ELEKEGIHSV GPTKSAAQIE TNKAFMRKLF EDYDIPGSIK 

       130        140        150        160        170        180 
YGTFYDLDEA YEFIDNFDEP VVVKPIGLTG GKGVKIVGDQ LADNDEAKEY VKEIFHQKMG 

       190        200        210        220        230        240 
GFEGVVIEEL LLGEEYTIQA FVDGTHLIPM PAAQDHPHAF VGNKGPITGG MGSYSDKNHL 

       250        260        270        280        290        300 
LPFLTQEDYD KSVEIMEKTI KAIAKEQEPY KGILYGQFML CKDGPKVIEY NARFGDPESM 

       310        320        330        340        350        360 
NVLSVIDDDL AKISKQIVDG TLDSVNFLNK SSVCKYVVPD KYPNTHVADT VVDVDEDKIN 

       370        380        390        400        410        420 
ELGAQVFYAA VYQDMDDSIK LTSSRALGVL AVKDSIKEAE KICEEAIKYV KGEVYHRNDV 

       430 
ATEELLNEKI KHMEEVRL 

« Hide

References

[1]"The genome sequence of Methanosphaera stadtmanae reveals why this human intestinal archaeon is restricted to methanol and H2 for methane formation and ATP synthesis."
Fricke W.F., Seedorf H., Henne A., Kruer M., Liesegang H., Hedderich R., Gottschalk G., Thauer R.K.
J. Bacteriol. 188:642-658(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000102 Genomic DNA. Translation: ABC56449.1.
RefSeqYP_447092.1. NC_007681.1.

3D structure databases

ProteinModelPortalQ2NI86.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING339860.Msp_0030.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABC56449; ABC56449; Msp_0030.
GeneID3855038.
KEGGmst:Msp_0030.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0151.
HOGENOMHOG000033464.
KOK01945.
OMAENLECEH.
ProtClustDBPRK00885.

Enzyme and pathway databases

BioCycMSTA339860:GJEZ-30-MONOMER.
UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_METST
AccessionPrimary (citable) accession number: Q2NI86
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 7, 2006
Last modified: February 19, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways