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Q2NI68

- PSB_METST

UniProt

Q2NI68 - PSB_METST

Protein

Proteasome subunit beta

Gene

psmB

Organism
Methanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 1 (07 Feb 2006)
      Previous versions | rss
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    Functioni

    Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation.UniRule annotation

    Catalytic activityi

    Cleavage of peptide bonds with very broad specificity.UniRule annotation

    Enzyme regulationi

    The formation of the proteasomal ATPase PAN-20S proteasome complex, via the docking of the C-termini of PAN into the intersubunit pockets in the alpha-rings, triggers opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei10 – 101NucleophileUniRule annotation

    GO - Molecular functioni

    1. threonine-type endopeptidase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. proteasomal protein catabolic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase, Protease, Threonine protease

    Enzyme and pathway databases

    BioCyciMSTA339860:GJEZ-282-MONOMER.

    Protein family/group databases

    MEROPSiT01.002.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proteasome subunit betaUniRule annotation (EC:3.4.25.1UniRule annotation)
    Alternative name(s):
    20S proteasome beta subunitUniRule annotation
    Proteasome core protein PsmBUniRule annotation
    Gene namesi
    Name:psmBUniRule annotation
    Ordered Locus Names:Msp_0282
    OrganismiMethanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3)
    Taxonomic identifieri339860 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanosphaera
    ProteomesiUP000001931: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. proteasome core complex, beta-subunit complex Source: UniProtKB-HAMAP

    Keywords - Cellular componenti

    Cytoplasm, Proteasome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – 99Removed in mature form; by autocatalysisUniRule annotationPRO_0000397374
    Chaini10 – 205196Proteasome subunit betaPRO_0000397375Add
    BLAST

    Keywords - PTMi

    Autocatalytic cleavage, Zymogen

    Interactioni

    Subunit structurei

    The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped at one or both ends by the proteasome regulatory ATPase, PAN.UniRule annotation

    Protein-protein interaction databases

    STRINGi339860.Msp_0282.

    Structurei

    3D structure databases

    ProteinModelPortaliQ2NI68.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase T1B family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0638.
    HOGENOMiHOG000091083.
    KOiK03433.
    OMAiGYYIAHR.

    Family and domain databases

    Gene3Di3.60.20.10. 1 hit.
    HAMAPiMF_02113_A. Proteasome_B_A.
    InterProiIPR029055. Ntn_hydrolases_N.
    IPR019983. Pept_T1A_Psome_bsu_arc.
    IPR000243. Pept_T1A_subB.
    IPR001353. Proteasome_sua/b.
    IPR023333. Proteasome_suB-type.
    [Graphical view]
    PfamiPF00227. Proteasome. 1 hit.
    [Graphical view]
    PRINTSiPR00141. PROTEASOME.
    SUPFAMiSSF56235. SSF56235. 1 hit.
    TIGRFAMsiTIGR03634. arc_protsome_B. 1 hit.
    PROSITEiPS51476. PROTEASOME_BETA_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q2NI68-1 [UniParc]FASTAAdd to Basket

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    MNQTENMEGT TTVGFVCTDG VVLATETRAT MGSLVANKNA DKLFQIDDKI    50
    GATIAGTVSH AQSLMDILKA EISLYKLRNE KDMSIDALAV LTSNILKSRP 100
    YYVQTILAGV DKDGAKLYTL DPSGSYIPDT FTSTGSGSPY AFGVLEDRYN 150
    EDITTEEGKK IAIKAITSAM ERDVYSGNNY RLGVITKDGM KIYTKEEIAQ 200
    IKKQL 205
    Length:205
    Mass (Da):22,360
    Last modified:February 7, 2006 - v1
    Checksum:iBD665B8926A37A62
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000102 Genomic DNA. Translation: ABC56692.1.
    RefSeqiYP_447335.1. NC_007681.1.

    Genome annotation databases

    EnsemblBacteriaiABC56692; ABC56692; Msp_0282.
    GeneIDi3855430.
    KEGGimst:Msp_0282.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000102 Genomic DNA. Translation: ABC56692.1 .
    RefSeqi YP_447335.1. NC_007681.1.

    3D structure databases

    ProteinModelPortali Q2NI68.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 339860.Msp_0282.

    Protein family/group databases

    MEROPSi T01.002.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABC56692 ; ABC56692 ; Msp_0282 .
    GeneIDi 3855430.
    KEGGi mst:Msp_0282.

    Phylogenomic databases

    eggNOGi COG0638.
    HOGENOMi HOG000091083.
    KOi K03433.
    OMAi GYYIAHR.

    Enzyme and pathway databases

    BioCyci MSTA339860:GJEZ-282-MONOMER.

    Family and domain databases

    Gene3Di 3.60.20.10. 1 hit.
    HAMAPi MF_02113_A. Proteasome_B_A.
    InterProi IPR029055. Ntn_hydrolases_N.
    IPR019983. Pept_T1A_Psome_bsu_arc.
    IPR000243. Pept_T1A_subB.
    IPR001353. Proteasome_sua/b.
    IPR023333. Proteasome_suB-type.
    [Graphical view ]
    Pfami PF00227. Proteasome. 1 hit.
    [Graphical view ]
    PRINTSi PR00141. PROTEASOME.
    SUPFAMi SSF56235. SSF56235. 1 hit.
    TIGRFAMsi TIGR03634. arc_protsome_B. 1 hit.
    PROSITEi PS51476. PROTEASOME_BETA_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Methanosphaera stadtmanae reveals why this human intestinal archaeon is restricted to methanol and H2 for methane formation and ATP synthesis."
      Fricke W.F., Seedorf H., Henne A., Kruer M., Liesegang H., Hedderich R., Gottschalk G., Thauer R.K.
      J. Bacteriol. 188:642-658(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3.

    Entry informationi

    Entry nameiPSB_METST
    AccessioniPrimary (citable) accession number: Q2NI68
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 10, 2010
    Last sequence update: February 7, 2006
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3