ID   TRM1_METST              Reviewed;         390 AA.
AC   Q2NI56;
DT   31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   07-FEB-2006, sequence version 1.
DT   16-JUN-2009, entry version 25.
DE   RecName: Full=N(2),N(2)-dimethylguanosine tRNA methyltransferase;
DE            EC=2.1.1.32;
DE   AltName: Full=tRNA(guanine-26,N(2)-N(2)) methyltransferase;
DE   AltName: Full=tRNA 2,2-dimethylguanosine-26 methyltransferase;
DE   AltName: Full=tRNA(m(2,2)G26)dimethyltransferase;
GN   Name=trm1; OrderedLocusNames=Msp_0291;
OS   Methanosphaera stadtmanae (strain DSM 3091).
OC   Archaea; Euryarchaeota; Methanobacteria; Methanobacteriales;
OC   Methanobacteriaceae; Methanosphaera.
OX   NCBI_TaxID=339860;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16385054; DOI=10.1128/JB.188.2.642-658.2006;
RA   Fricke W.F., Seedorf H., Henne A., Kruer M., Liesegang H.,
RA   Hedderich R., Gottschalk G., Thauer R.K.;
RT   "The genome sequence of Methanosphaera stadtmanae reveals why this
RT   human intestinal archaeon is restricted to methanol and H2 for methane
RT   formation and ATP synthesis.";
RL   J. Bacteriol. 188:642-658(2006).
CC   -!- FUNCTION: Dimethylates a single guanine residue at position 26 of
CC       a number of tRNAs using S-adenosyl-L-methionine as donor of the
CC       methyl groups (By similarity).
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + tRNA = S-adenosyl-L-
CC       homocysteine + tRNA containing N(2)-methylguanine.
CC   -!- SIMILARITY: Belongs to the TRM1 family.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; CP000102; ABC56701.1; -; Genomic_DNA.
DR   RefSeq; YP_447344.1; -.
DR   GeneID; 3854938; -.
DR   GenomeReviews; CP000102_GR; Msp_0291.
DR   KEGG; mst:Msp_0291; -.
DR   NMPDR; fig|339860.6.peg.282; -.
DR   HOGENOM; Q2NI56; -.
DR   OMA; Q2NI56; MELNRDI.
DR   BioCyc; MSTA339860:MSP_0291-MON; -.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0004809; F:tRNA (guanine-N2-)-methyltransferase activity; IEA:HAMAP.
DR   GO; GO:0006400; P:tRNA modification; IEA:HAMAP.
DR   HAMAP; MF_00290; -; 1.
DR   InterPro; IPR002905; TRM_MeTrfase.
DR   PANTHER; PTHR10631; TRM_mtfrase; 1.
DR   Pfam; PF02005; TRM; 1.
DR   TIGRFAMs; TIGR00308; TRM1; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Methyltransferase; S-adenosyl-L-methionine;
KW   Transferase; tRNA processing.
FT   CHAIN         1    390       N(2),N(2)-dimethylguanosine tRNA
FT                                methyltransferase.
FT                                /FTId=PRO_0000259376.
SQ   SEQUENCE   390 AA;  44038 MW;  46E86601C5ED2FB9 CRC64;
     MDTHIIEEGL VKIEVPDFEK VSAKAPVFYN PVMEMNRDIS VAVINQYRKE VDHDISICDA
     FGGTGIRGAR YSKEIHGVEK VVVGDVNPLA VEISKKNMQL NNISNVEVQK NDANILLQSN
     KGLFDVVDID PFGTPAMFTQ STAANIRPGG LICISATDTS ALCGTYHDPC LRKYGAKPLK
     TEYCHENGIR ILIAFISRNL AVNQKYLHVL FSHSTEHYMR VYAIVKRGGK KTNKALDNIG
     FIAHCPNCLH RETTYGFAPK IPHTCPECGG EYDVAGPLWL GKIWDKEFIY NTMELVKNLN
     LNKKDDLMSL FEKCYMEADG PVTFYDIHKI CKKLKISSPK INDVMDEIRN RGYFISRTHF
     KLTGMRTDMP LDELKQLILD IKEDKLGPEK
//