Q2NI56 (TRM1_METST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine(26)-N(2))-dimethyltransferase
EC=2.1.1.216

Alternative name(s):
tRNA 2,2-dimethylguanosine-26 methyltransferase
tRNA(guanine-26,N(2)-N(2)) methyltransferase
tRNA(m(2,2)G26)dimethyltransferase

Gene names

Name:	trm1
Ordered Locus Names:	Msp_0291

Organism

Methanosphaera stadtmanae (strain DSM 3091) [Complete proteome] [HAMAP]

Taxonomic identifier

339860 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanosphaera

Protein attributes

Sequence length	390 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Dimethylates a single guanine residue at position 26 of a number of tRNAs using S-adenosyl-L-methionine as donor of the methyl groups By similarity. HAMAP MF_00290
Catalytic activity	2 S-adenosyl-L-methionine + guanine(26) in tRNA = 2 S-adenosyl-L-homocysteine + N(2)-dimethylguanine(26) in tRNA. HAMAP MF_00290
Sequence similarities	Belongs to the TRM1 family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N2-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 390	390	tRNA (guanine(26)-N(2))-dimethyltransferase HAMAP MF_00290		PRO_0000259376

Sequences

	Sequence		Length	Mass (Da)
	Q2NI56 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 46E86601C5ED2FB9	FASTA	390	44,038
	10 20 30 40 50 60 MDTHIIEEGL VKIEVPDFEK VSAKAPVFYN PVMEMNRDIS VAVINQYRKE VDHDISICDA 70 80 90 100 110 120 FGGTGIRGAR YSKEIHGVEK VVVGDVNPLA VEISKKNMQL NNISNVEVQK NDANILLQSN 130 140 150 160 170 180 KGLFDVVDID PFGTPAMFTQ STAANIRPGG LICISATDTS ALCGTYHDPC LRKYGAKPLK 190 200 210 220 230 240 TEYCHENGIR ILIAFISRNL AVNQKYLHVL FSHSTEHYMR VYAIVKRGGK KTNKALDNIG 250 260 270 280 290 300 FIAHCPNCLH RETTYGFAPK IPHTCPECGG EYDVAGPLWL GKIWDKEFIY NTMELVKNLN 310 320 330 340 350 360 LNKKDDLMSL FEKCYMEADG PVTFYDIHKI CKKLKISSPK INDVMDEIRN RGYFISRTHF 370 380 390 KLTGMRTDMP LDELKQLILD IKEDKLGPEK

References

[1]

"The genome sequence of Methanosphaera stadtmanae reveals why this human intestinal archaeon is restricted to methanol and H2 for methane formation and ATP synthesis." Fricke W.F., Seedorf H., Henne A., Kruer M., Liesegang H., Hedderich R., Gottschalk G., Thauer R.K. J. Bacteriol. 188:642-658(2006) [PubMed: 16385054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 3091.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000102 Genomic DNA. Translation: ABC56701.1.
RefSeq	YP_447344.1. NC_007681.1.
3D structure databases
ProteinModelPortal	Q2NI56.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2NI56.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3854938.
GenomeReviews	Gene locus Msp_0291 in contig CP000102_GR.
KEGG	mst:Msp_0291.
NMPDR	fig|339860.6.peg.282.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04822.
HOGENOM	HBG577872.
OMA	DAPVFYN.
PhylomeDB	Q2NI56.
ProtClustDB	PRK04338.
Enzyme and pathway databases
BioCyc	MSTA339860:MSP_0291-MONOMER.
Family and domain databases
HAMAP	MF_00290. tRNA_dimethyltr_TRM1. [Tree]
InterPro	IPR022923. DimeGuo_tRNA_meTrfase_arc. IPR002905. TRM_MeTrfase. [Graphical view]
KO	K00555.
PANTHER	PTHR10631. TRM_mtfrase. 1 hit.
Pfam	PF02005. TRM. 1 hit. [Graphical view]
TIGRFAMs	TIGR00308. TRM1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRM1_METST

Accession

Primary (citable) accession number: Q2NI56

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	February 7, 2006
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program