Q2NHW2 (DAPA_METST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydrodipicolinate synthase
Short name=DHDPS
EC=4.2.1.52

Gene names

Name:	dapA
Ordered Locus Names:	Msp_0108

Organism

Methanosphaera stadtmanae (strain DSM 3091) [Complete proteome] [HAMAP]

Taxonomic identifier

339860 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanosphaera

Protein attributes

Sequence length	297 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418
Subcellular location	Cytoplasm By similarity HAMAP MF_00418.
Sequence similarities	Belongs to the DHDPS family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydrodipicolinate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 297	297	Dihydrodipicolinate synthase HAMAP MF_00418		PRO_1000050223
Regions
	Region	49 – 50	2	Pyruvate binding By similarity
Sites
	Active site	162	1	Schiff-base intermediate with substrate By similarity
	Binding site	107	1	Pyruvate By similarity
	Site	134	1	Involved in proton transfer during cleavage By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2NHW2 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 348BFD78983CC9F1	FASTA	297	32,319
	10 20 30 40 50 60 MNLEGTHVAM ITPFNNDNTI NEEKYREFID FLIEGGVDGI LAAGTTGESA TLTLEEHQKV 70 80 90 100 110 120 IDIMVDQANG RVTTIAGAGS NATSEALDLV NYSKDAGADV ALVITPYYNK PQQSGLYNHF 130 140 150 160 170 180 KLLNDQCDMP IIAYNVPSRT GVDLSVDNII NLAKLDNIVA IKEANPDLNK LAHVFSRLNS 190 200 210 220 230 240 ENLLDDFTVL SGNDSLTLPM ISQGSKGVIS VVANIMPNKT STMVNNALNG NYDEARTLSN 250 260 270 280 290 ELFNLMDVLF IEASPAPTKR ALNLMGMDVG GLRMPINEIC DENEVILKEI LKENNLI

References

[1]

"The genome sequence of Methanosphaera stadtmanae reveals why this human intestinal archaeon is restricted to methanol and H2 for methane formation and ATP synthesis." Fricke W.F., Seedorf H., Henne A., Kruer M., Liesegang H., Hedderich R., Gottschalk G., Thauer R.K. J. Bacteriol. 188:642-658(2006) [PubMed: 16385054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 3091.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000102 Genomic DNA. Translation: ABC56527.1.
RefSeq	YP_447170.1. NC_007681.1.
3D structure databases
HSSP	HSSP built from PDB template 1O5K based on UniProtKB Q9X1K9.
ProteinModelPortal	Q2NHW2.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2NHW2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3855388.
GenomeReviews	Gene locus Msp_0108 in contig CP000102_GR.
KEGG	mst:Msp_0108.
NMPDR	fig|339860.6.peg.106.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG05039.
HOGENOM	HBG358848.
OMA	ITRELCG.
PhylomeDB	Q2NHW2.
ProtClustDB	PRK03170.
Enzyme and pathway databases
BioCyc	MSTA339860:MSP_0108-MONOMER.
Family and domain databases
HAMAP	MF_00418. DapA. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR002220. Dihydrodipicolinate_synth-like. IPR020625. Dihydrodipicolinate_synth_AS. IPR020624. Dihydrodipicolinate_synth_CS. IPR005263. Dihydrodipicolinate_synth_DapA. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01714.
PANTHER	PTHR12128. DHDPS. 1 hit.
Pfam	PF00701. DHDPS. 1 hit. [Graphical view]
PIRSF	PIRSF001365. DHDPS. 1 hit.
PRINTS	PR00146. DHPICSNTHASE.
TIGRFAMs	TIGR00674. DapA. 1 hit.
PROSITE	PS00665. DHDPS_1. 1 hit. PS00666. DHDPS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPA_METST

Accession

Primary (citable) accession number: Q2NHW2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 7, 2006
Last modified:	November 16, 2011
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program