Q2NHP5 (Q2NHP5_METST) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase HAMAP MF_00193 RuleBase RU003812
EC=6.3.1.5 HAMAP MF_00193 RuleBase RU003812

Gene names

Name:	nadE HAMAP MF_00193 EMBL ABC56588.1
Ordered Locus Names:	Msp_0170

Organism

Methanosphaera stadtmanae (strain DSM 3091) [Complete proteome] [HAMAP]

Taxonomic identifier

339860 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanosphaera

Protein attributes

Sequence length	265 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193 RuleBase RU003812
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. RuleBase RU004252 HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family. HAMAP MF_00193 RuleBase RU003811

Ontologies

Keywords
Ligand	ATP-binding HAMAP MF_00193 RuleBase RU003811 NAD HAMAP MF_00193 RuleBase RU003811 Nucleotide-binding
Molecular function	Ligase HAMAP MF_00193 RuleBase RU003811 EMBL ABC56588.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	35 – 42	8	ATP By similarity HAMAP MF_00193
Sites
	Active site	37	1	By similarity HAMAP MF_00193

Sequences

	Sequence		Length	Mass (Da)
	Q2NHP5 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: D5F06C78AAF2BCF7	FASTA	265	29,749
	10 20 30 40 50 60 MIDLPEFDAR KFIERACEFI KEKVDESNSD GVVIGLSGGI DSCVVACLAV RALGPLRVRG 70 80 90 100 110 120 YILPTITTSD QDLYDAKLIK DELDIESEYI SIGSIYDEFI SSCEIKNLPQ DNINLARGNL 130 140 150 160 170 180 KPRIRMSILY YYATIYNSLV IGTGNKTELQ VGYFTKHGDG GVDLLPIGDL YKMDVKKVAQ 190 200 210 220 230 240 ELGVPSLIIK KPPTAGLWEG QTDEEELGMT YNILDKLLYL YLEEEYSMPD IAKELEIPES 250 260 EVERIINMVN NASHKRNKIP ILSKN

References

[1]

"The genome sequence of Methanosphaera stadtmanae reveals why this human intestinal archaeon is restricted to methanol and H2 for methane formation and ATP synthesis." Fricke W.F., Seedorf H., Henne A., Kruer M., Liesegang H., Hedderich R., Gottschalk G., Thauer R.K. J. Bacteriol. 188:642-658(2006) [PubMed: 16385054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000102 Genomic DNA. Translation: ABC56588.1.
RefSeq	YP_447231.1. NC_007681.1.
3D structure databases
ProteinModelPortal	Q2NHP5.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2NHP5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3856044.
GenomeReviews	Gene locus Msp_0170 in contig CP000102_GR.
KEGG	mst:Msp_0170.
NMPDR	fig|339860.6.peg.168.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG08797.
HOGENOM	HBG351567.
OMA	YDISARD.
PhylomeDB	Q2NHP5.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

Q2NHP5_METST

Accession

Primary (citable) accession number: Q2NHP5

Entry history

Integrated into UniProtKB/TrEMBL:	February 7, 2006
Last sequence update:	February 7, 2006
Last modified:	December 14, 2011
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)