Reviewed, UniProtKB/Swiss-Prot Q2NHG2 (DP2L_METST)

Names and origin

Protein names

Recommended name:
    DNA polymerase II large subunit
      Short name=Pol II
    EC=2.7.7.7

Gene names

Name:	polC
Ordered Locus Names:	Msp_0255

Organism

Methanosphaera stadtmanae (strain DSM 3091) [Complete proteome] [HAMAP]

Taxonomic identifier

339860 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanosphaera

Protein attributes

Sequence length	1117 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase By similarity. HAMAP MF_00324
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_00324 Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates. HAMAP MF_00324
Subunit structure	Heterodimer of a large subunit and a small subunit By similarity. HAMAP MF_00324
Sequence similarities	Belongs to the archaeal DNA polymerase II family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	DNA-binding
Molecular function	DNA-directed DNA polymerase Exonuclease Hydrolase Nuclease Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	DNA catabolic process, exonucleolytic Inferred from electronic annotation. Source: HAMAP DNA-dependent DNA replication Inferred from electronic annotation. Source: HAMAP
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: HAMAP DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1117	1117	DNA polymerase II large subunit HAMAP MF_00324		PRO_0000294693

Sequences

	Sequence		Length	Mass (Da)
	Q2NHG2-1 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: DE1855748F768A1F	FASTA	1,117	124,988
	10 20 30 40 50 60 MDYFGMLEEK TKELYAIARE ARKQGKDLEL EPEIPLAKDL AERVEGLVGP EGVAKRIKEL 70 80 90 100 110 120 EKSMSREEVA FQIAKEIATK DDVEGQPNDY EVQEANADSA IRTALAILTE GVVAAPLEGI 130 140 150 160 170 180 AKVKIKDNSD GTKCFGVYFA GPIRSAGGTA AALAVLLGDY IRMSQGLDRY KPTDDEIERY 190 200 210 220 230 240 VEEVELYESE VTNLQYSPTP DEVRLAIRGI PVEVTGEQTD PVEVQNRDLP RVETNNLRGG 250 260 270 280 290 300 ALLAVAEGVI QKSRKIVKYA KTLKIDGWDW LEYFTAPKST KEEEKKKEES SENKPKKKAK 310 320 330 340 350 360 YMEDIIGGRP VMSYPGAKGG FRLRYGRTRD SGLASMAIHP ATMEIVEFLA IGTQMKIEKP 370 380 390 400 410 420 GKGNCVVPCD SIEGPIVKLK NGDVIQVNDV SKAISIRRDV NEIIFLGDML VAFGEYLRGN 430 440 450 460 470 480 IPLDVSAWCE EWWAQEIEAS EYFKETHDTF GIDFNENMEL NALLKLDIDA KKAFDIAKKT 490 500 510 520 530 540 NTPLHPKFTF YYNDVTKEEL NDLHNYLYSL IDSPEDVFKS DMNRIPIDYH KRIIEVLGIP 550 560 570 580 590 600 HHVNNKSLIM SNDNLYTLMS VLNKSLSPDE DMETIEAVNM ISPVSIKSKA PTYIGGRVGR 610 620 630 640 650 660 PEKTKERLMK PAPHSLFPIG NYAGNIRNIV EAAKKGTIKV TLAKCKCTNP DCKVSSFKAL 670 680 690 700 710 720 CPVCGSRTEL ESSAAKNIKL DKLLMDAFEN VNVRRLDEVK GVKGLISEDK YPEPLEKGIL 730 740 750 760 770 780 RARNDVFTYR DGTIRHDSTD LPLTHFIPRE VGVPYEKILE MGYTEDIYGK PITNDNQIIE 790 800 810 820 830 840 IKIQDIVVSE SCGDYLLKVS KFIDDLLIRY YHMEPFYNAE NRVDLVGHLI AGLAPHTSAG 850 860 870 880 890 900 VLGRIVGFTK ASCCYAHPYF HSAKRRNCDS DEDAVMLLLD ALLNFGKTYL PSTRGGSMDA 910 920 930 940 950 960 PLVLSIRIDP EEIDDESHNI DCMKRIPLEI YHKTEEGGVK PSDVNDLVDN VESRLGTDNQ 970 980 990 1000 1010 1020 YHGLMYSHPT SSIHAGPKIC LYKTLQTMTD KVESQIALAE LLRAVDQKGV VEGVLNSHFL 1030 1040 1050 1060 1070 1080 PDMAGNIRAF SRQKVRCTKC GAKYRRIPLS GECTCGNNLI LSISKGSVLK YLDISKDLSH 1090 1100 1110 RYPINPYVVE RIEILETGIN SLFESDKSKQ SSLDAFF

References

[1]

"The genome sequence of Methanosphaera stadtmanae reveals why this human intestinal archaeon is restricted to methanol and H2 for methane formation and ATP synthesis." Fricke W.F., Seedorf H., Henne A., Kruer M., Liesegang H., Hedderich R., Gottschalk G., Thauer R.K. J. Bacteriol. 188:642-658(2006) [PubMed: 16385054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000102 Genomic DNA. Translation: ABC56671.1.
RefSeq	YP_447314.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2NHG2.
Genome annotation databases
GeneID	3855689.
GenomeReviews	Gene locus Msp_0255 in contig CP000102_GR.
KEGG	mst:Msp_0255.
NMPDR	fig|339860.6.peg.251.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04008.
HOGENOM	HBG497074.
OMA	NCDGDED.
Enzyme and pathway databases
BioCyc	MSTA339860:MSP_0255-MONOMER.
Family and domain databases
HAMAP	MF_00324. DNApol_II_L_arch. [Tree]
InterPro	IPR004475. PolC_DP2. IPR016033. PolC_DP2_N. [Graphical view]
Pfam	PF03833. PolC_DP2. 1 hit. [Graphical view]
PIRSF	PIRSF016275. PolC_DP2. 1 hit.
TIGRFAMs	TIGR00354. polC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DP2L_METST

Accession

Primary (citable) accession number: Q2NHG2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	February 7, 2006
Last modified:	February 9, 2010
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)