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Protein

tRNA pseudouridine synthase A

Gene

truA

Organism
Methanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs.UniRule annotation

Catalytic activityi

tRNA uridine(38-40) = tRNA pseudouridine(38-40).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei56 – 561NucleophileUniRule annotation
Binding sitei109 – 1091SubstrateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

BioCyciMSTA339860:GJEZ-388-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA pseudouridine synthase AUniRule annotation (EC:5.4.99.12UniRule annotation)
Alternative name(s):
tRNA pseudouridine(38-40) synthaseUniRule annotation
tRNA pseudouridylate synthase IUniRule annotation
tRNA-uridine isomerase IUniRule annotation
Gene namesi
Name:truAUniRule annotation
Ordered Locus Names:Msp_0388
OrganismiMethanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3)
Taxonomic identifieri339860 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanosphaera
Proteomesi
  • UP000001931 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 274274tRNA pseudouridine synthase APRO_1000194583Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi339860.Msp_0388.

Structurei

3D structure databases

ProteinModelPortaliQ2NHB4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA pseudouridine synthase TruA family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04449. Archaea.
COG0101. LUCA.
HOGENOMiHOG000248673.
KOiK06173.
OMAiIRTPIMA.

Family and domain databases

Gene3Di3.30.70.580. 1 hit.
3.30.70.660. 1 hit.
HAMAPiMF_00171. TruA.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR001406. PsdUridine_synth_TruA.
IPR020097. PsdUridine_synth_TruA_a/b_dom.
IPR020095. PsdUridine_synth_TruA_C.
IPR020094. PsdUridine_synth_TruA_N.
[Graphical view]
PANTHERiPTHR11142. PTHR11142. 1 hit.
PfamiPF01416. PseudoU_synth_1. 1 hit.
[Graphical view]
PIRSFiPIRSF001430. tRNA_psdUrid_synth. 1 hit.
SUPFAMiSSF55120. SSF55120. 1 hit.
TIGRFAMsiTIGR00071. hisT_truA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2NHB4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRVALKIAY IGSNFHGYQR QPNYRTVEGE LLRVFKETNI IEDTWTAHYS
60 70 80 90 100
VAGRTDKGVH STGNVISFIT DEDIHINQLN GLLPDDIKII GEARVPYGFK
110 120 130 140 150
VRFPLTRTYT YIQPISPFEK KNLDITKMHV AMESFIGKHN FRNFSKRNEK
160 170 180 190 200
NPNRKIIDVN LEVDEDVLIF TIVGESFLWN MVRKMVTSIM EVGYGKLDIN
210 220 230 240 250
DINELLKPKE LRQFIRLQPA PANGLILSDM EYKNIKFKDS EYAKNKLVEF
260 270
LKKEYMLHEQ EKKADCRLIK ILKK
Length:274
Mass (Da):32,045
Last modified:February 7, 2006 - v1
Checksum:iC1D5FCEC709DAB4B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000102 Genomic DNA. Translation: ABC56789.1.
RefSeqiWP_011405989.1. NC_007681.1.

Genome annotation databases

EnsemblBacteriaiABC56789; ABC56789; Msp_0388.
GeneIDi3855753.
KEGGimst:Msp_0388.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000102 Genomic DNA. Translation: ABC56789.1.
RefSeqiWP_011405989.1. NC_007681.1.

3D structure databases

ProteinModelPortaliQ2NHB4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi339860.Msp_0388.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABC56789; ABC56789; Msp_0388.
GeneIDi3855753.
KEGGimst:Msp_0388.

Phylogenomic databases

eggNOGiarCOG04449. Archaea.
COG0101. LUCA.
HOGENOMiHOG000248673.
KOiK06173.
OMAiIRTPIMA.

Enzyme and pathway databases

BioCyciMSTA339860:GJEZ-388-MONOMER.

Family and domain databases

Gene3Di3.30.70.580. 1 hit.
3.30.70.660. 1 hit.
HAMAPiMF_00171. TruA.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR001406. PsdUridine_synth_TruA.
IPR020097. PsdUridine_synth_TruA_a/b_dom.
IPR020095. PsdUridine_synth_TruA_C.
IPR020094. PsdUridine_synth_TruA_N.
[Graphical view]
PANTHERiPTHR11142. PTHR11142. 1 hit.
PfamiPF01416. PseudoU_synth_1. 1 hit.
[Graphical view]
PIRSFiPIRSF001430. tRNA_psdUrid_synth. 1 hit.
SUPFAMiSSF55120. SSF55120. 1 hit.
TIGRFAMsiTIGR00071. hisT_truA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Methanosphaera stadtmanae reveals why this human intestinal archaeon is restricted to methanol and H2 for methane formation and ATP synthesis."
    Fricke W.F., Seedorf H., Henne A., Kruer M., Liesegang H., Hedderich R., Gottschalk G., Thauer R.K.
    J. Bacteriol. 188:642-658(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3.

Entry informationi

Entry nameiTRUA_METST
AccessioniPrimary (citable) accession number: Q2NHB4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 7, 2006
Last modified: May 11, 2016
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.