Q2NHB3 (HIS4_METST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase
EC=5.3.1.16

Alternative name(s):
Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Gene names

Name:	hisA
Ordered Locus Names:	Msp_0389

Organism

Methanosphaera stadtmanae (strain DSM 3091) [Complete proteome] [HAMAP]

Taxonomic identifier

339860 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanosphaera › Methanosphaera stadtmanae

Protein attributes

Sequence length	237 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide = 5-((5-phospho-1-deoxyribulos-1-ylamino)methylideneamino)-1-(5-phosphoribosyl)imidazole-4-carboxamide. HAMAP-Rule MF_01014
Pathway	Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 4/9. HAMAP-Rule MF_01014
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the HisA/HisF family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	histidine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 237	237	1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase HAMAP-Rule MF_01014		PRO_0000290577
Sites
	Active site	8	1	Proton acceptor By similarity
	Active site	129	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2NHB3 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: F242EB90A7F7872E	FASTA	237	25,627
	10 20 30 40 50 60 MIIIPAVDIK NGKCVQLVQG EPGTEQVVIE NPAEVAKQWE QKGAKKLHIV DLDGALGSGR 70 80 90 100 110 120 NLSIVKEIIE KTKSPIQLGG GIRSVSDAKK LLEIGVNTVI IGTMAIKNPE IIKELSSEYG 130 140 150 160 170 180 CERICVSLDS KNNKVVTHGW KESTDKTPIE YAKLFEKMGA GSILFTNVDV EGLLNGINLE 190 200 210 220 230 PIKELLNNVS IPIIYSGGIT SVDDLKVLSD IGVDYVVIGS ALYKGLITLE DALKYQK

References

[1]

"The genome sequence of Methanosphaera stadtmanae reveals why this human intestinal archaeon is restricted to methanol and H2 for methane formation and ATP synthesis." Fricke W.F., Seedorf H., Henne A., Kruer M., Liesegang H., Hedderich R., Gottschalk G., Thauer R.K. J. Bacteriol. 188:642-658(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 3091.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000102 Genomic DNA. Translation: ABC56790.1.
RefSeq	YP_447433.1. NC_007681.1.
3D structure databases
HSSP	HSSP built from PDB template 1VZW based on UniProtKB P16250.
ProteinModelPortal	Q2NHB3.
ModBase	Search...
Protein-protein interaction databases
STRING	339860.Msp_0389.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABC56790; ABC56790; Msp_0389.
GeneID	3855754.
KEGG	mst:Msp_0389.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0106.
HOGENOM	HOG000224614.
KO	K01814.
OMA	CARYVVT.
ProtClustDB	PRK13585.
Enzyme and pathway databases
BioCyc	MSTA339860:GJEZ-389-MONOMER.
UniPathway	UPA00031; UER00009.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_01014. HisA.
InterPro	IPR013785. Aldolase_TIM. IPR006062. His_biosynth. IPR006063. HisA. IPR023016. Isoase_HisA. IPR011060. RibuloseP-bd_barrel. [Graphical view]
Pfam	PF00977. His_biosynth. 1 hit. [Graphical view]
SUPFAM	SSF51366. RibP_bind_barrel. 1 hit.
TIGRFAMs	TIGR00007. TIGR00007. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HIS4_METST

Accession

Primary (citable) accession number: Q2NHB3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 12, 2007
Last sequence update:	February 7, 2006
Last modified:	May 1, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program