Q2NH05 (AROA_METST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable 3-phosphoshikimate 1-carboxyvinyltransferase
EC=2.5.1.19

Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthase
Short name=EPSP synthase
Short name=EPSPS

Gene names

Name:	aroA
Ordered Locus Names:	Msp_0498

Organism

Methanosphaera stadtmanae (strain DSM 3091) [Complete proteome] [HAMAP]

Taxonomic identifier

339860 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanosphaera

Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate. HAMAP MF_00210
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7. HAMAP MF_00210
Subunit structure	Monomer By similarity. HAMAP MF_00210
Subcellular location	Cytoplasm By similarity HAMAP MF_00210.
Sequence similarities	Belongs to the EPSP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 426	426	Probable 3-phosphoshikimate 1-carboxyvinyltransferase HAMAP MF_00210		PRO_0000325407

Sequences

	Sequence		Length	Mass (Da)
	Q2NH05 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 06272D26D63141BB	FASTA	426	46,559
	10 20 30 40 50 60 MDLQVEKINK ISGVIKAPAS KSYSHRAFIA AALAEGQSIL RDPLYSADTI ATLEACEQLG 70 80 90 100 110 120 ALFQRYPDKC IVQGTAGYIR TPENIIDVKN SGTSVRILSS VAAIAPRANY TIFTGDESLR 130 140 150 160 170 180 KRPMQDLIDA LENLGVQISS SQSNGTPPII VKGGFEGGQT DIKGDVSSQF ISSIIMAAPY 190 200 210 220 230 240 SKKPVTLNVK GTFVSKPYVN MTLSVISKFG IDFEYDTTNI PEYSSYYIEP QKYEATDYTI 250 260 270 280 290 300 EGDYSSASYI LAAAAMLPSN LTVKNLYKDS MQGDKIIIDI IKKMGAEVTV DDDQIHIESD 310 320 330 340 350 360 GNLKAFDINL EDAPDLLPTI SILMAQAEGV SKITGVEHAR FKETDRVHNC AVELENVGVD 370 380 390 400 410 420 VEELQDGLII KGNPTGGYVD SHMDHRMVMA FYVLGLKIGN IIIKDASCYD ISFPNFLEVM HTISEE

References

[1]

"The genome sequence of Methanosphaera stadtmanae reveals why this human intestinal archaeon is restricted to methanol and H2 for methane formation and ATP synthesis." Fricke W.F., Seedorf H., Henne A., Kruer M., Liesegang H., Hedderich R., Gottschalk G., Thauer R.K. J. Bacteriol. 188:642-658(2006) [PubMed: 16385054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 3091.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000102 Genomic DNA. Translation: ABC56898.1.
RefSeq	YP_447541.1. NC_007681.1.
3D structure databases
HSSP	HSSP built from PDB template 2AA9 based on UniProtKB P0A6D3.
ProteinModelPortal	Q2NH05.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2NH05.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3856185.
GenomeReviews	Gene locus Msp_0498 in contig CP000102_GR.
KEGG	mst:Msp_0498.
NMPDR	fig|339860.6.peg.480.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG05032.
HOGENOM	HBG646626.
OMA	GADIEWG.
PhylomeDB	Q2NH05.
ProtClustDB	PRK02427.
Enzyme and pathway databases
BioCyc	MSTA339860:MSP_0498-MONOMER.
Family and domain databases
HAMAP	MF_00210. EPSP_synth. [Tree]
InterPro	IPR001986. Enolpyruvate_Tfrase_dom. IPR006264. EPSP_synthase. IPR023193. EPSP_synthase_CS. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
KO	K00800.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000505. EPSPS. 1 hit.
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR01356. AroA. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. 1 hit. PS00885. EPSP_SYNTHASE_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA_METST

Accession

Primary (citable) accession number: Q2NH05

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	February 7, 2006
Last modified:	November 16, 2011
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program