Reviewed, UniProtKB/Swiss-Prot Q2NGS8 (AROD_METST)

Names and origin

Protein names

Recommended name:
    3-dehydroquinate dehydratase
      Short name=3-dehydroquinase
    EC=4.2.1.10
Alternative name(s):
    Type I DHQase

Gene names

Name:	aroD
Ordered Locus Names:	Msp_0577

Organism

Methanosphaera stadtmanae (strain DSM 3091) [Complete proteome] [HAMAP]

Taxonomic identifier

339860 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanosphaera

Protein attributes

Sequence length	235 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	3-dehydroquinate = 3-dehydroshikimate + H2O. HAMAP MF_00214
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7. HAMAP MF_00214
Sequence similarities	Belongs to the type-I 3-dehydroquinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	3-dehydroquinate dehydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 235	235	3-dehydroquinate dehydratase HAMAP MF_00214		PRO_0000325539
Sites
	Active site	133	1	Proton acceptor By similarity
	Active site	159	1	Schiff-base intermediate with substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2NGS8-1 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 21701FC949F51023	FASTA	235	26,697
	10 20 30 40 50 60 MIFFLLEGER EIITEICGSI IESNEKDIFK TLDKAVDLDV NYIELRLDVI ENITSKKATS 70 80 90 100 110 120 IIEKIRNVTD IPIILTNRTK YEGGYFPSTE EDRIKILMDN ASLVEYTDIE LSTNENLRQE 130 140 150 160 170 180 VIDNANRTII SYHNFEKTPS SEYLENVVNS ALNVGDIAKI AVKPLNIEDT YVLLRLLMEY 190 200 210 220 230 DDLIGISMDK LGSYTRILGP ILGSPVTYTA ITDESAPGQF DVKTTRDILK KLKNY

References

[1]

"The genome sequence of Methanosphaera stadtmanae reveals why this human intestinal archaeon is restricted to methanol and H2 for methane formation and ATP synthesis." Fricke W.F., Seedorf H., Henne A., Kruer M., Liesegang H., Hedderich R., Gottschalk G., Thauer R.K. J. Bacteriol. 188:642-658(2006) [PubMed: 16385054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000102 Genomic DNA. Translation: ABC56975.1.
RefSeq	YP_447618.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2NGS8.
Genome annotation databases
GeneID	3854828.
GenomeReviews	Gene locus Msp_0577 in contig CP000102_GR.
KEGG	mst:Msp_0577.
NMPDR	fig|339860.6.peg.557.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2NGS8.
OMA	IAKLAVM.
Enzyme and pathway databases
BioCyc	MSTA339860:MSP_0577-MON.
Family and domain databases
HAMAP	MF_00214. [Tree]
InterPro	IPR018508. 3-dehydroquinate_DH_AS. IPR013785. Aldolase_TIM. IPR001381. DHquinase_I. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01487. DHquinase_I. 1 hit. [Graphical view]
ProDom	PD005337. DHquinase_I. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01093. aroD. 1 hit.
PROSITE	PS01028. DEHYDROQUINASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROD_METST

Accession

Primary (citable) accession number: Q2NGS8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	February 7, 2006
Last modified:	November 3, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)