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Protein

Hydroxyethylthiazole kinase 1

Gene

thiM1

Organism
Methanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathway: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Hydroxyethylthiazole kinase 1 (thiM1), Hydroxyethylthiazole kinase 2 (thiM2)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei48 – 481Substrate; via amide nitrogenUniRule annotation
Binding sitei124 – 1241ATPUniRule annotation
Binding sitei183 – 1831ATPUniRule annotation
Binding sitei210 – 2101Substrate; via amide nitrogenUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMSTA339860:GJEZ-682-MONOMER.
UniPathwayiUPA00060; UER00139.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinase 1UniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase 1UniRule annotation
Short name:
TH kinase 1UniRule annotation
Short name:
Thz kinase 1UniRule annotation
Gene namesi
Name:thiM1UniRule annotation
Ordered Locus Names:Msp_0682
OrganismiMethanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3)
Taxonomic identifieri339860 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanosphaera
ProteomesiUP000001931 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 285285Hydroxyethylthiazole kinase 1PRO_0000383918Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi339860.Msp_0682.

Structurei

3D structure databases

ProteinModelPortaliQ2NGH3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2145.
HOGENOMiHOG000114352.
KOiK00878.
OMAiSPVMAHA.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2NGH3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEYTLENIA QVVEILREKS PLTHCITNVV TVKDCANAVL AVGASPIMAN
60 70 80 90 100
APEEAEEIVG ISNSLVINIG TLTKEQIETM KKSAKFAIEN NKPFILDPVG
110 120 130 140 150
VGISNIRNQT PIDIITNSKP SIIRGNLSEI KAIAMMYDIL DECTMAKGVD
160 170 180 190 200
VAQCDIINKD TLISNCNLIK NISEKLNTTI AVSGPIDIIS DGHDVYTIEN
210 220 230 240 250
GDAMMSRITG SGCMLGCVLG AYLAVTNPLE AAITGTLVMG IAGELAAKTA
260 270 280
RDNNKGTGSF GIYLIDELSK LNKSTILSQS KLNKM
Length:285
Mass (Da):30,378
Last modified:February 7, 2006 - v1
Checksum:i60F0BF4DAA247275
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000102 Genomic DNA. Translation: ABC57080.1.
RefSeqiWP_011406280.1. NC_007681.1.
YP_447723.1. NC_007681.1.

Genome annotation databases

EnsemblBacteriaiABC57080; ABC57080; Msp_0682.
GeneIDi3855870.
KEGGimst:Msp_0682.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000102 Genomic DNA. Translation: ABC57080.1.
RefSeqiWP_011406280.1. NC_007681.1.
YP_447723.1. NC_007681.1.

3D structure databases

ProteinModelPortaliQ2NGH3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi339860.Msp_0682.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABC57080; ABC57080; Msp_0682.
GeneIDi3855870.
KEGGimst:Msp_0682.

Phylogenomic databases

eggNOGiCOG2145.
HOGENOMiHOG000114352.
KOiK00878.
OMAiSPVMAHA.

Enzyme and pathway databases

UniPathwayiUPA00060; UER00139.
BioCyciMSTA339860:GJEZ-682-MONOMER.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Methanosphaera stadtmanae reveals why this human intestinal archaeon is restricted to methanol and H2 for methane formation and ATP synthesis."
    Fricke W.F., Seedorf H., Henne A., Kruer M., Liesegang H., Hedderich R., Gottschalk G., Thauer R.K.
    J. Bacteriol. 188:642-658(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3.

Entry informationi

Entry nameiTHIM1_METST
AccessioniPrimary (citable) accession number: Q2NGH3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: February 7, 2006
Last modified: May 27, 2015
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.