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Protein

Enolase

Gene

eno

Organism
Methanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.UniRule annotation

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gap)
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (apgM)
  4. Enolase (eno)
  5. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei147SubstrateUniRule annotation1
Binding sitei157SubstrateUniRule annotation1
Active sitei200Proton donorUniRule annotation1
Metal bindingi236MagnesiumUniRule annotation1
Metal bindingi281MagnesiumUniRule annotation1
Binding sitei281SubstrateUniRule annotation1
Metal bindingi308MagnesiumUniRule annotation1
Binding sitei308SubstrateUniRule annotation1
Active sitei333Proton acceptorUniRule annotation1
Binding sitei333Substrate (covalent); in inhibited formUniRule annotation1
Binding sitei384SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
EnolaseUniRule annotation (EC:4.2.1.11UniRule annotation)
Alternative name(s):
2-phospho-D-glycerate hydro-lyaseUniRule annotation
2-phosphoglycerate dehydrataseUniRule annotation
Gene namesi
Name:enoUniRule annotation
Ordered Locus Names:Msp_0862
OrganismiMethanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3)
Taxonomic identifieri339860 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanosphaera
Proteomesi
  • UP000001931 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Secreted UniRule annotation
  • Cell surface UniRule annotation

  • Note: Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface.UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002671431 – 414EnolaseAdd BLAST414

Interactioni

Protein-protein interaction databases

STRINGi339860.Msp_0862.

Structurei

3D structure databases

ProteinModelPortaliQ2NG02.
SMRiQ2NG02.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni360 – 363Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the enolase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01169. Archaea.
COG0148. LUCA.
HOGENOMiHOG000072173.
KOiK01689.
OMAiYVEDPFH.
OrthoDBiPOG093Z03D9.

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR034390. Enolase-like_superfamily.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SFLDiSFLDG00178. enolase. 1 hit.
SFLDS00001. Enolase. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiView protein in PROSITE
PS00164. ENOLASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2NG02-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSVIEDVRL RKIIDSRGNP TVEADVLTWN GFGRAAAPSG ASTGINEVTS
60 70 80 90 100
FPDGGVDQVI TDVEDLISSE IIGMEAEDIR EIDNVLKEID GTDNFSTIGG
110 120 130 140 150
NTAVAVSMAT AKAAASSYNL PLYKFLGGIM PTQIPFPLGN MINGGAHAGT
160 170 180 190 200
NAPDIQEFLV IPVGASNITE AITTNINVHR RIKAKIQEKD KTFTGGKGDE
210 220 230 240 250
GGWAPNLTNE EALEIQFTSC EEVSDETGVL VKPGLDVASS EFWNEKEQKY
260 270 280 290 300
VYEREGTSRT VEEQIDYIAD LIDTYKFFYV EDPIQENDFE AFAELTSKSG
310 320 330 340 350
KDCLICGDDL FVTNAEILAK GIEAHAGNSL IIKPNQIGTL TDTYNTIKLA
360 370 380 390 400
KANKYVPVVS HRSGETTDET IAHLAVAFNA PIIKTGAAGG ERIAKLNELV
410
RIEEELLNPS MADL
Length:414
Mass (Da):44,699
Last modified:February 7, 2006 - v1
Checksum:i5DD7000DE2D74EF4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000102 Genomic DNA. Translation: ABC57251.1.
RefSeqiWP_011406450.1. NC_007681.1.

Genome annotation databases

EnsemblBacteriaiABC57251; ABC57251; Msp_0862.
GeneIDi3854736.
KEGGimst:Msp_0862.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiENO_METST
AccessioniPrimary (citable) accession number: Q2NG02
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: February 7, 2006
Last modified: June 7, 2017
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families