Q2NFM9 (PURA_METST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenylosuccinate synthetase
Short name=AMPSase
Short name=AdSS
EC=6.3.4.4

Alternative name(s):
IMP--aspartate ligase

Gene names

Name:	purA
Ordered Locus Names:	Msp_0986

Organism

Methanosphaera stadtmanae (strain DSM 3091) [Complete proteome] [HAMAP]

Taxonomic identifier

339860 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanosphaera

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP By similarity. HAMAP MF_00011
Catalytic activity	GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP. HAMAP MF_00011
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00011
Pathway	Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. HAMAP MF_00011
Subunit structure	Homodimer By similarity. HAMAP MF_00011
Subcellular location	Cytoplasm By similarity HAMAP MF_00011.
Sequence similarities	Belongs to the adenylosuccinate synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylosuccinate synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 341	341	Adenylosuccinate synthetase HAMAP MF_00011		PRO_1000000861
Regions
	Nucleotide binding	12 – 18	7	GTP By similarity
	Nucleotide binding	42 – 44	3	GTP By similarity
	Nucleotide binding	284 – 286	3	GTP By similarity
	Nucleotide binding	324 – 326	3	GTP By similarity
	Region	13 – 16	4	IMP binding By similarity
	Region	40 – 43	4	IMP binding By similarity
	Region	252 – 258	7	Substrate binding By similarity
Sites
	Active site	13	1	Proton acceptor By similarity
	Active site	43	1	Proton donor By similarity
	Metal binding	13	1	Magnesium By similarity
	Metal binding	42	1	Magnesium; via carbonyl oxygen By similarity
	Binding site	127	1	IMP By similarity
	Binding site	141	1	IMP; shared with dimeric partner By similarity
	Binding site	179	1	IMP By similarity
	Binding site	194	1	IMP By similarity
	Binding site	256	1	IMP By similarity
	Binding site	258	1	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2NFM9 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 716072FEF3FC1DD3	FASTA	341	37,346
	10 20 30 40 50 60 MTCTILVGGQ WGDEGKGKCI TYFCTQDKPE IIARAGVGPN AGHSVEFNGE KYGLRLTPSG 70 80 90 100 110 120 FFNKEARLLI GAGVLVNPEV FEHELEYLSK YAVEGRTFMD ANCAIITDKH TKQDKDSAYL 130 140 150 160 170 180 SKKIGTTGSG CGPANADRIN RTIDYAKDVP ELEKYITDVP SEINKAIDEG KDVFIEGSQG 190 200 210 220 230 240 FGLSLYYGTY PYVTSKDTCA STAAADVGVG PTKVDEVIVI FKSYITRVGE GPFPTEISPE 250 260 270 280 290 300 EAEKMGIEEY GVVTGRKRRV GLFDKDFAKR SCMINGATQI ALTCIDRLYP ECAKVNKYED 310 320 330 340 LSQEARDYIE DIEENVGVPI TIISTGPDLA DTIDLRNEKL N

References

[1]

"The genome sequence of Methanosphaera stadtmanae reveals why this human intestinal archaeon is restricted to methanol and H2 for methane formation and ATP synthesis." Fricke W.F., Seedorf H., Henne A., Kruer M., Liesegang H., Hedderich R., Gottschalk G., Thauer R.K. J. Bacteriol. 188:642-658(2006) [PubMed: 16385054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 3091.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000102 Genomic DNA. Translation: ABC57374.1.
RefSeq	YP_448017.1. NC_007681.1.
3D structure databases
ProteinModelPortal	Q2NFM9.
SMR	Q2NFM9. Positions 1-337.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2NFM9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3855974.
GenomeReviews	Gene locus Msp_0986 in contig CP000102_GR.
KEGG	mst:Msp_0986.
NMPDR	fig|339860.6.peg.945.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04146.
HOGENOM	HBG658237.
OMA	IDPHVGI.
ProtClustDB	PRK04293.
Enzyme and pathway databases
BioCyc	MSTA339860:MSP_0986-MONOMER.
Family and domain databases
HAMAP	MF_00011. Adenylosucc_synth. [Tree]
InterPro	IPR018220. Adenylosuccinate_synthase_AS. IPR001114. Adenylosuccinate_synthetase. [Graphical view]
KO	K01939.
PANTHER	PTHR11846. Asucc_synthtase. 1 hit.
Pfam	PF00709. Adenylsucc_synt. 3 hits. [Graphical view]
SMART	SM00788. Adenylsucc_synt. 1 hit. [Graphical view]
PROSITE	PS01266. ADENYLOSUCCIN_SYN_1. 1 hit. PS00513. ADENYLOSUCCIN_SYN_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURA_METST

Accession

Primary (citable) accession number: Q2NFM9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 7, 2006
Last modified:	November 16, 2011
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program