Q2NFL1 (SYP_METST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	Msp_1004

Organism

Methanosphaera stadtmanae (strain DSM 3091) [Complete proteome] [HAMAP]

Taxonomic identifier

339860 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanosphaera

Protein attributes

Sequence length	469 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity. HAMAP MF_01571
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01571
Subunit structure	Homodimer By similarity. HAMAP MF_01571
Subcellular location	Cytoplasm By similarity HAMAP MF_01571.
Domain	Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension By similarity. HAMAP MF_01571
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW proline-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 469	469	Proline--tRNA ligase HAMAP MF_01571		PRO_0000249163

Sequences

	Sequence		Length	Mass (Da)
	Q2NFL1 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 93378A1173BE701A	FASTA	469	54,332
	10 20 30 40 50 60 MKNFSEWFHN ILEEAELMDA RYPIKGMSVW LPRGFQIRKY ALNALQELLD KDHEEVLFPM 70 80 90 100 110 120 LIPQSELAKE AIHVKGFEEE VYWVTKGGKR DLNEHLALRP TSETSIYPMF SLWVRSHMDL 130 140 150 160 170 180 PIKVYQTVNT FRYETKHTRP LIRVREITTF NETHTAHATE EEAEKEVMEG IEIYKTFFDE 190 200 210 220 230 240 LGIPYSISKR PEWDKFPGSK YTMAFDMIMP DGKTLQIATV HNLGTTFAHT FDIQFENEDG 250 260 270 280 290 300 THDYVHQVCY GLSDRVIASL IAAHGDEKGL SLPPVVAPEQ VIIIPIIFKE NQDVVLNFTD 310 320 330 340 350 360 NLEKLLKNNG IRVKQDKREL RPGKKYYEWE KRGVPLRIEV GPRDIENNTI VINRRDTGDK 370 380 390 400 410 420 EFVDYDENTI VDVVKDRLDS ITHDMKEASN KFQEEKTFAI EKPEQIKKTI NKKGGIITCS 430 440 450 460 WCGETDCGKD MEEKFDIDVL GTQESDLENK TCINCGKDAS YKVLISKTY

References

[1]

"The genome sequence of Methanosphaera stadtmanae reveals why this human intestinal archaeon is restricted to methanol and H2 for methane formation and ATP synthesis." Fricke W.F., Seedorf H., Henne A., Kruer M., Liesegang H., Hedderich R., Gottschalk G., Thauer R.K. J. Bacteriol. 188:642-658(2006) [PubMed: 16385054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 3091.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000102 Genomic DNA. Translation: ABC57392.1.
RefSeq	YP_448035.1. NC_007681.1.
3D structure databases
ProteinModelPortal	Q2NFL1.
SMR	Q2NFL1. Positions 4-469.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2NFL1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3855285.
GenomeReviews	Gene locus Msp_1004 in contig CP000102_GR.
KEGG	mst:Msp_1004.
NMPDR	fig|339860.6.peg.963.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04466.
HOGENOM	HBG334108.
OMA	KFAEYEL.
PhylomeDB	Q2NFL1.
ProtClustDB	PRK08661.
Enzyme and pathway databases
BioCyc	MSTA339860:MSP_1004-MONOMER.
Family and domain databases
HAMAP	MF_01571. Pro_tRNA_synth_type3. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-synth_IIa. IPR004499. Pro-tRNA-synth_IIa_arc-type. IPR017449. Pro-tRNA_synth_II. IPR016061. Pro-tRNA_synth_II_C. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.30.110.30. Pro-tRNA-synth_II_C_arc/euk. 1 hit.
KO	K01881.
PANTHER	PTHR11451:SF6. ProS_fam_I. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF09180. ProRS-C_1. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SMART	SM00946. ProRS-C_1. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF64586. Pro-tRNA_synth_II_C. 1 hit.
TIGRFAMs	TIGR00408. ProS_fam_I. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_METST

Accession

Primary (citable) accession number: Q2NFL1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	February 7, 2006
Last modified:	January 25, 2012
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program