Q2NF74 (Q2NF74_METST) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Probable L-aspartate dehydrogenase HAMAP MF_01265
EC=1.4.1.21 HAMAP MF_01265

Gene names

Name:	nadX HAMAP MF_01265
Ordered Locus Names:	Msp_1148

Organism

Methanosphaera stadtmanae (strain DSM 3091) [Complete proteome] [HAMAP]

Taxonomic identifier

339860 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanosphaera

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically catalyzes the NAD or NADP-dependent dehydrogenation of L-aspartate to iminoaspartate By similarity. HAMAP MF_01265
Catalytic activity	L-aspartate + H2O + NAD(P)+ = oxaloacetate + NH3 + NAD(P)H. HAMAP MF_01265
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (dehydrogenase route): step 1/1. HAMAP MF_01265
Miscellaneous	The iminoaspartate product is unstable in aqueous solution and can decompose to oxaloacetate and ammonia By similarity. HAMAP MF_01265
Sequence similarities	Belongs to the L-aspartate dehydrogenase family. HAMAP MF_01265

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis HAMAP MF_01265
Ligand	NAD HAMAP MF_01265 NADP HAMAP MF_01265
Molecular function	Oxidoreductase HAMAP MF_01265
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP NADP catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	NAD binding Inferred from electronic annotation. Source: HAMAP NADP binding Inferred from electronic annotation. Source: HAMAP aspartate dehydrogenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	207	1	By similarity HAMAP MF_01265
	Binding site	121	1	NAD; via amide nitrogen By similarity HAMAP MF_01265
	Binding site	179	1	NAD By similarity HAMAP MF_01265

Sequences

	Sequence		Length	Mass (Da)
	Q2NF74 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 134C69D85C1A461E	FASTA	256	27,521
	10 20 30 40 50 60 MKIGIMGCGS IANTLTNFQL NGKLNVKLSY FYDINRNNAI NLAKKVDAEV VDTIEELIER 70 80 90 100 110 120 SDLILEAAAQ SAVRNSVPKI LENGVNVIIM SVGALMDSEF KNHLERLADE SNVKIYLPTG 130 140 150 160 170 180 AITGIDTVKA STMGEIESIS LTTRKPPVSL GMTLEGNEEK VLFEGKASEA VNKFPKNINV 190 200 210 220 230 240 SSTLSLASGI DVDVKIIADP KIDKNTHEIH LKGSFGELIT RTANVSSPDN PKTSMLAAFS 250 AACLLNKLSK TIQIGS

References

[1]

"The genome sequence of Methanosphaera stadtmanae reveals why this human intestinal archaeon is restricted to methanol and H2 for methane formation and ATP synthesis." Fricke W.F., Seedorf H., Henne A., Kruer M., Liesegang H., Hedderich R., Gottschalk G., Thauer R.K. J. Bacteriol. 188:642-658(2006) [PubMed: 16385054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000102 Genomic DNA. Translation: ABC57529.1.
RefSeq	YP_448172.1. NC_007681.1.
3D structure databases
ProteinModelPortal	Q2NF74.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2NF74.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3855543.
GenomeReviews	Gene locus Msp_1148 in contig CP000102_GR.
KEGG	mst:Msp_1148.
NMPDR	fig|339860.6.peg.1099.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG05209.
HOGENOM	HBG649642.
OMA	ECAGHSA.
ProtClustDB	PRK13304.
Family and domain databases
HAMAP	MF_01265. NadX. [Tree]
InterPro	IPR005106. Asp/hSer_DH_NAD-bd. IPR002811. Asp_DH. IPR011182. Asp_DH_NAD_syn. IPR020626. Asp_DH_NAD_syn_prok. IPR022487. Asp_DH_NAD_synth_arc. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K06989.
Pfam	PF01958. DUF108. 1 hit. PF03447. NAD_binding_3. 1 hit. [Graphical view]
PIRSF	PIRSF005227. Asp_dh_NAD_syn. 1 hit.
TIGRFAMs	TIGR03855. NAD_NadX. 1 hit.
ProtoNet	Search...

Entry information

Entry name

Q2NF74_METST

Accession

Primary (citable) accession number: Q2NF74

Entry history

Integrated into UniProtKB/TrEMBL:	February 7, 2006
Last sequence update:	February 7, 2006
Last modified:	November 16, 2011
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)