Q2NF42 (GSA_METST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate-1-semialdehyde 2,1-aminomutase
Short name=GSA
EC=5.4.3.8

Alternative name(s):
Glutamate-1-semialdehyde aminotransferase
Short name=GSA-AT

Gene names

Name:	hemL
Ordered Locus Names:	Msp_1180

Organism

Methanosphaera stadtmanae (strain DSM 3091) [Complete proteome] [HAMAP]

Taxonomic identifier

339860 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanosphaera › Methanosphaera stadtmanae

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-4-amino-5-oxopentanoate = 5-aminolevulinate. HAMAP-Rule MF_00375
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 2/2. HAMAP-Rule MF_00375
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00375.
Sequence similarities	Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: HAMAP protoporphyrinogen IX biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glutamate-1-semialdehyde 2,1-aminomutase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transaminase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 423	423	Glutamate-1-semialdehyde 2,1-aminomutase HAMAP-Rule MF_00375		PRO_0000243648
Amino acid modifications
	Modified residue	262	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2NF42 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 3EE1A2409FBC9E95	FASTA	423	46,757
	10 20 30 40 50 60 MKLDKSKELF DEAVNYLPGG VNSPVRAYKP YPFFAKSAKG SKIYDVDGNE YIDYCLGYGP 70 80 90 100 110 120 LLFGHANEHI IEKSIEQLKL GTDYGVPSEK EVQLAKEVIK RVPCAQMVRF TNSGTEATMS 130 140 150 160 170 180 AIRLARGITK RDKIIKFEGA YHGAHDAVLV KSGSGAAGLP DSPGIPTDTT KNTMLVEFND 190 200 210 220 230 240 EEALKKLINE NKDEIACIIV EPVMGNIGCV PPKEGYLDFL REITLENGIL LIIDEVITGF 250 260 270 280 290 300 RISKGGAQEY YNVIPDLATF GKIVGGGFPI GAIAGKKEYM EQFTPSGKIY QAGTFSGNPM 310 320 330 340 350 360 SINGGLAAFE VLDDNSYKQL HKSGEYFRNG IVDILDKLNI NFHVNGVESM TQIYFTENEV 370 380 390 400 410 420 YDYKTAQTSD TENFLKYFHL LLENGVFIAP SQYECGFIST SHSRDDLDKT LNAIEIALTK LYK

References

[1]

"The genome sequence of Methanosphaera stadtmanae reveals why this human intestinal archaeon is restricted to methanol and H2 for methane formation and ATP synthesis." Fricke W.F., Seedorf H., Henne A., Kruer M., Liesegang H., Hedderich R., Gottschalk G., Thauer R.K. J. Bacteriol. 188:642-658(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 3091.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000102 Genomic DNA. Translation: ABC57561.1.
RefSeq	YP_448204.1. NC_007681.1.
3D structure databases
ProteinModelPortal	Q2NF42.
ModBase	Search...
Protein-protein interaction databases
STRING	339860.Msp_1180.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABC57561; ABC57561; Msp_1180.
GeneID	3855237.
KEGG	mst:Msp_1180.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0001.
HOGENOM	HOG000020210.
KO	K01845.
OMA	FNGNPIS.
ProtClustDB	PRK00062.
Enzyme and pathway databases
BioCyc	MSTA339860:GJEZ-1180-MONOMER.
UniPathway	UPA00251; UER00317.
Family and domain databases
Gene3D	3.40.640.10. 1 hit. 3.90.1150.10. 2 hits.
HAMAP	MF_00375. HemL_aminotrans_3.
InterPro	IPR004639. 4pyrrol_synth_GluAld_NH2Trfase. IPR005814. Aminotrans_3. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
PANTHER	PTHR11986. PTHR11986. 1 hit. PTHR11986:SF5. PTHR11986:SF5. 1 hit.
Pfam	PF00202. Aminotran_3. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
TIGRFAMs	TIGR00713. hemL. 1 hit.
PROSITE	PS00600. AA_TRANSFER_CLASS_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSA_METST

Accession

Primary (citable) accession number: Q2NF42

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	February 7, 2006
Last modified:	May 1, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program