Q2NEW4 (SYA_METST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine--tRNA ligase
EC=6.1.1.7

Alternative name(s):
Alanyl-tRNA synthetase
Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	Msp_1262

Organism

Methanosphaera stadtmanae (strain DSM 3091) [Complete proteome] [HAMAP]

Taxonomic identifier

339860 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanosphaera

Protein attributes

Sequence length	903 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036_A
Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036_A
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036_A
Subcellular location	Cytoplasm By similarity HAMAP MF_00036_A.
Domain	Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036_A
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alanine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 903	903	Alanine--tRNA ligase HAMAP MF_00036_A		PRO_0000347888
Sites
	Metal binding	591	1	Zinc By similarity
	Metal binding	595	1	Zinc By similarity
	Metal binding	695	1	Zinc By similarity
	Metal binding	699	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2NEW4 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 832CC37BC029CE3D	FASTA	903	102,117
	10 20 30 40 50 60 MTYELEQLGY KKQVCQKCGN TFWSIRERAT CGDAPCDEYE FIGNPVTDKQ YDLMGIQKKF 70 80 90 100 110 120 KGFFKDHGHT PINRYPVLAK RWRNDVFLVG ASIYDFQPWV TSGMVRPPAN PLVVAQPSIR 130 140 150 160 170 180 LNDVDNVGRT GRHMTCFTMG AHHAFNTDET PIYWKNETLR YCHEFLVSIG INPEEITYIE 190 200 210 220 230 240 SWWKGGGNEG PSFEICAHGV ELATLVFIQY ATTKDGLKEI PLKIVDTGYG LERIAWVSQG 250 260 270 280 290 300 TPTAYDATFG SVIDKLTDIS GVELNTEILS ENARIAGMMD IEDISDLKLL RKKVADKLSL 310 320 330 340 350 360 DPDMLKKTTA PMEAIYIVAD HTRCLSFMLA DGIIPSNVKE GYLARLVLRR TVKYMNELGL 370 380 390 400 410 420 NESLSDIMKM QVDYLSKTYP EIKDNKDHII NITDLEEERY HTTLTKGKNL VKRSIKTLKK 430 440 450 460 470 480 QNKKSFPTDM LINFYDSHGI PPETVEAISK ENAFDANIPD NFYTQIAAAH EEEEEEEIEE 490 500 510 520 530 540 MELNFPKTKL SFYDDLKQRT FTAKVLGVVD SNKIILNQTI YYPEGGGQPS DIGTITRVNG 550 560 570 580 590 600 EVLNITYAQK VDGIVLHHVA KEDESKLDNI VGEEITGEID SKRRDLLTRN HTATHLVIAS 610 620 630 640 650 660 AREVLGKHIW QAGAQKGLDK TRIDLSHYKR ISHEEVQEIE RLANKRVQEN HPVNIQWYDR 670 680 690 700 710 720 TDAEVKYGFK LYQGGIVPGK NIRVVEIPGI DVQACAGTHC EKTGDIGVIK LLRTERVQDG 730 740 750 760 770 780 VERLEYAVSD SGIKKIQDDD DIIRNSSDVF GVDAEQLPRT CKRFFNEWKE QQKRIKSLEK 790 800 810 820 830 840 QLAQVKIFSL ENEIANINGY NVITEVLDVD NNQLREIAIN LVEKEEVADI AILINNNGNI 850 860 870 880 890 900 VASSNNKILE KGMKMGDVVN DIGKFLGGRG GGKPTLAQGA KMTDLSRKDE AFESVKEQIR SWN

References

[1]

"The genome sequence of Methanosphaera stadtmanae reveals why this human intestinal archaeon is restricted to methanol and H2 for methane formation and ATP synthesis." Fricke W.F., Seedorf H., Henne A., Kruer M., Liesegang H., Hedderich R., Gottschalk G., Thauer R.K. J. Bacteriol. 188:642-658(2006) [PubMed: 16385054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 3091.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000102 Genomic DNA. Translation: ABC57639.1.
RefSeq	YP_448282.1. NC_007681.1.
3D structure databases
ProteinModelPortal	Q2NEW4.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2NEW4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3855828.
GenomeReviews	Gene locus Msp_1262 in contig CP000102_GR.
KEGG	mst:Msp_1262.
NMPDR	fig|339860.6.peg.1209.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04130.
HOGENOM	HBG392147.
OMA	MFTNSGM.
PhylomeDB	Q2NEW4.
ProtClustDB	PRK13902.
Enzyme and pathway databases
BioCyc	MSTA339860:MSP_1262-MONOMER.
Family and domain databases
HAMAP	MF_00036_A. Ala_tRNA_synth_A. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR022429. Ala-tRNA_synth_arc. IPR003156. Pesterase_DHHA1. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
KO	K01872.
PANTHER	PTHR11777:SF6. PTHR11777:SF6. 1 hit.
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR03683. A-tRNA_syn_arch. 1 hit. TIGR00344. AlaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_METST

Accession

Primary (citable) accession number: Q2NEW4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	February 7, 2006
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program