Q2NER5 (GUAAA_METST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP synthase [glutamine-hydrolyzing] subunit A
EC=6.3.5.2

Alternative name(s):
Glutamine amidotransferase

Gene names

Name:	guaAA
Ordered Locus Names:	Msp_1311

Organism

Methanosphaera stadtmanae (strain DSM 3091) [Complete proteome] [HAMAP]

Taxonomic identifier

339860 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanosphaera

Protein attributes

Sequence length	186 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_01510
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_01510
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_01510
Subunit structure	Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B) Potential.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 186	186	GMP synthase [glutamine-hydrolyzing] subunit A HAMAP MF_01510		PRO_0000292197
Regions
	Domain	2 – 186	185	Glutamine amidotransferase type-1
Sites
	Active site	76	1	Nucleophile By similarity
	Active site	163	1	By similarity
	Active site	165	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2NER5 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 608D3625C5D10B41	FASTA	186	20,948
	10 20 30 40 50 60 MSIVIINNFG QYNHRISRTL KYLNIENSLI PNTTSFEEIE QMNPKGIILG GGPSIERIGN 70 80 90 100 110 120 CKEIILNMDI PILGICLGHQ IIADVFGGET KSAEIESYAQ IELNILKENG LFKGIGDSLK 130 140 150 160 170 180 VWASHKDEVV TLPENFEILA NSDKCDIEAM KHEDKNIYGI QFHPEVQHTP RGGEIFENFN KICENY

References

[1]

"The genome sequence of Methanosphaera stadtmanae reveals why this human intestinal archaeon is restricted to methanol and H2 for methane formation and ATP synthesis." Fricke W.F., Seedorf H., Henne A., Kruer M., Liesegang H., Hedderich R., Gottschalk G., Thauer R.K. J. Bacteriol. 188:642-658(2006) [PubMed: 16385054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 3091.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000102 Genomic DNA. Translation: ABC57688.1.
RefSeq	YP_448331.1. NC_007681.1.
3D structure databases
ProteinModelPortal	Q2NER5.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2NER5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3855939.
GenomeReviews	Gene locus Msp_1311 in contig CP000102_GR.
KEGG	mst:Msp_1311.
NMPDR	fig|339860.6.peg.1257.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG05587.
HOGENOM	HBG292341.
OMA	GQYVHRI.
ProtClustDB	PRK00758.
Enzyme and pathway databases
BioCyc	MSTA339860:MSP_1311-MONOMER.
Family and domain databases
HAMAP	MF_01510. GMP_synthase_A. [Tree]
InterPro	IPR017926. GATASE_1. IPR004739. GMP_synth_N. IPR023686. GMP_synthase_A. [Graphical view]
KO	K01951.
Pfam	PF00117. GATase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00888. GuaA_Nterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAAA_METST

Accession

Primary (citable) accession number: Q2NER5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	February 7, 2006
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program