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Q2NEP6

- NADK_METST

UniProt

Q2NEP6 - NADK_METST

Protein

NAD kinase

Gene

nadK

Organism
Methanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 55 (01 Oct 2014)
      Sequence version 1 (07 Feb 2006)
      Previous versions | rss
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    Functioni

    Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.UniRule annotation

    Catalytic activityi

    ATP + NAD+ = ADP + NADP+.UniRule annotation

    Cofactori

    Divalent metal ions.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei66 – 661Proton acceptorUniRule annotation
    Binding sitei71 – 711NADUniRule annotation
    Binding sitei146 – 1461NADUniRule annotation
    Binding sitei163 – 1631NADUniRule annotation
    Binding sitei165 – 1651NADUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi66 – 672NADUniRule annotation
    Nucleotide bindingi135 – 1362NADUniRule annotation
    Nucleotide bindingi176 – 1816NADUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-HAMAP
    3. NAD+ kinase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. NAD metabolic process Source: InterPro
    2. NADP biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, NAD, NADP, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMSTA339860:GJEZ-1331-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NAD kinaseUniRule annotation (EC:2.7.1.23UniRule annotation)
    Alternative name(s):
    ATP-dependent NAD kinaseUniRule annotation
    Gene namesi
    Name:nadKUniRule annotation
    Ordered Locus Names:Msp_1330
    OrganismiMethanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3)
    Taxonomic identifieri339860 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanosphaera
    ProteomesiUP000001931: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 275275NAD kinasePRO_1000059881Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi339860.Msp_1330.

    Structurei

    3D structure databases

    ProteinModelPortaliQ2NEP6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the NAD kinase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0061.
    HOGENOMiHOG000227222.
    KOiK00858.
    OMAiGPILHES.

    Family and domain databases

    Gene3Di2.60.200.30. 1 hit.
    3.40.50.10330. 1 hit.
    HAMAPiMF_00361. NAD_kinase.
    InterProiIPR017438. ATP-NAD_kinase_dom_1.
    IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
    IPR002504. PolyP/ATP_NADK.
    [Graphical view]
    PANTHERiPTHR20275. PTHR20275. 1 hit.
    PfamiPF01513. NAD_kinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF111331. SSF111331. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q2NEP6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRIGIISRTD KEEAIELDDT IIKYLFENNI EVELDSQLTK KLPQYSENSV    50
    DITKMNADIV LCVGGDGTVL HAQRYLSPKK IPILSINMGT VGFLTEVDPE 100
    DIFECLDKLL SYDFFIEERL QLDVLIDSQW HTVLNELVLM TSQPAKMLDL 150
    RVSVDEEIVD EVRADGLIIS TPSGSTAYAM SAGGPIVDPR VDAAIIIPIC 200
    PFKLNTRPKI VPADSIITVK FLKEGKKGVA VLDGIVNKEF DYLGEIKLKK 250
    SENSAYFVRF KKNFYNSVNN KLIVG 275
    Length:275
    Mass (Da):30,748
    Last modified:February 7, 2006 - v1
    Checksum:iF4338F5D7F6B56B8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000102 Genomic DNA. Translation: ABC57707.1.
    RefSeqiWP_011406906.1. NC_007681.1.
    YP_448350.1. NC_007681.1.

    Genome annotation databases

    EnsemblBacteriaiABC57707; ABC57707; Msp_1330.
    GeneIDi3855653.
    KEGGimst:Msp_1330.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000102 Genomic DNA. Translation: ABC57707.1 .
    RefSeqi WP_011406906.1. NC_007681.1.
    YP_448350.1. NC_007681.1.

    3D structure databases

    ProteinModelPortali Q2NEP6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 339860.Msp_1330.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABC57707 ; ABC57707 ; Msp_1330 .
    GeneIDi 3855653.
    KEGGi mst:Msp_1330.

    Phylogenomic databases

    eggNOGi COG0061.
    HOGENOMi HOG000227222.
    KOi K00858.
    OMAi GPILHES.

    Enzyme and pathway databases

    BioCyci MSTA339860:GJEZ-1331-MONOMER.

    Family and domain databases

    Gene3Di 2.60.200.30. 1 hit.
    3.40.50.10330. 1 hit.
    HAMAPi MF_00361. NAD_kinase.
    InterProi IPR017438. ATP-NAD_kinase_dom_1.
    IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
    IPR002504. PolyP/ATP_NADK.
    [Graphical view ]
    PANTHERi PTHR20275. PTHR20275. 1 hit.
    Pfami PF01513. NAD_kinase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF111331. SSF111331. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Methanosphaera stadtmanae reveals why this human intestinal archaeon is restricted to methanol and H2 for methane formation and ATP synthesis."
      Fricke W.F., Seedorf H., Henne A., Kruer M., Liesegang H., Hedderich R., Gottschalk G., Thauer R.K.
      J. Bacteriol. 188:642-658(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3.

    Entry informationi

    Entry nameiNADK_METST
    AccessioniPrimary (citable) accession number: Q2NEP6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: February 7, 2006
    Last modified: October 1, 2014
    This is version 55 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3