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Q2NEP6

- NADK_METST

UniProt

Q2NEP6 - NADK_METST

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Protein

NAD kinase

Gene

nadK

Organism
Methanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.UniRule annotation

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei66 – 661Proton acceptorUniRule annotation
Binding sitei71 – 711NADUniRule annotation
Binding sitei146 – 1461NADUniRule annotation
Binding sitei163 – 1631NADUniRule annotation
Binding sitei165 – 1651NADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi66 – 672NADUniRule annotation
Nucleotide bindingi135 – 1362NADUniRule annotation
Nucleotide bindingi176 – 1816NADUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-HAMAP
  3. NAD+ kinase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. NAD metabolic process Source: InterPro
  2. NADP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciMSTA339860:GJEZ-1331-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinaseUniRule annotation (EC:2.7.1.23UniRule annotation)
Alternative name(s):
ATP-dependent NAD kinaseUniRule annotation
Gene namesi
Name:nadKUniRule annotation
Ordered Locus Names:Msp_1330
OrganismiMethanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3)
Taxonomic identifieri339860 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanosphaera
ProteomesiUP000001931: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 275275NAD kinasePRO_1000059881Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi339860.Msp_1330.

Structurei

3D structure databases

ProteinModelPortaliQ2NEP6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000227222.
KOiK00858.
OMAiGPILHES.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2NEP6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRIGIISRTD KEEAIELDDT IIKYLFENNI EVELDSQLTK KLPQYSENSV
60 70 80 90 100
DITKMNADIV LCVGGDGTVL HAQRYLSPKK IPILSINMGT VGFLTEVDPE
110 120 130 140 150
DIFECLDKLL SYDFFIEERL QLDVLIDSQW HTVLNELVLM TSQPAKMLDL
160 170 180 190 200
RVSVDEEIVD EVRADGLIIS TPSGSTAYAM SAGGPIVDPR VDAAIIIPIC
210 220 230 240 250
PFKLNTRPKI VPADSIITVK FLKEGKKGVA VLDGIVNKEF DYLGEIKLKK
260 270
SENSAYFVRF KKNFYNSVNN KLIVG
Length:275
Mass (Da):30,748
Last modified:February 7, 2006 - v1
Checksum:iF4338F5D7F6B56B8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000102 Genomic DNA. Translation: ABC57707.1.
RefSeqiYP_448350.1. NC_007681.1.

Genome annotation databases

EnsemblBacteriaiABC57707; ABC57707; Msp_1330.
GeneIDi3855653.
KEGGimst:Msp_1330.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000102 Genomic DNA. Translation: ABC57707.1 .
RefSeqi YP_448350.1. NC_007681.1.

3D structure databases

ProteinModelPortali Q2NEP6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 339860.Msp_1330.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABC57707 ; ABC57707 ; Msp_1330 .
GeneIDi 3855653.
KEGGi mst:Msp_1330.

Phylogenomic databases

eggNOGi COG0061.
HOGENOMi HOG000227222.
KOi K00858.
OMAi GPILHES.

Enzyme and pathway databases

BioCyci MSTA339860:GJEZ-1331-MONOMER.

Family and domain databases

Gene3Di 2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPi MF_00361. NAD_kinase.
InterProi IPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view ]
PANTHERi PTHR20275. PTHR20275. 1 hit.
Pfami PF01513. NAD_kinase. 1 hit.
[Graphical view ]
SUPFAMi SSF111331. SSF111331. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of Methanosphaera stadtmanae reveals why this human intestinal archaeon is restricted to methanol and H2 for methane formation and ATP synthesis."
    Fricke W.F., Seedorf H., Henne A., Kruer M., Liesegang H., Hedderich R., Gottschalk G., Thauer R.K.
    J. Bacteriol. 188:642-658(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3.

Entry informationi

Entry nameiNADK_METST
AccessioniPrimary (citable) accession number: Q2NEP6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: February 7, 2006
Last modified: November 26, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3