Q2NEB6 (PURL_METST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	Msp_1467

Organism

Methanosphaera stadtmanae (strain DSM 3091) [Complete proteome] [HAMAP]

Taxonomic identifier

339860 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanosphaera

Protein attributes

Sequence length	723 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL.
Subcellular location	Cytoplasm By similarity HAMAP MF_00420.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 723	723	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_0000236676
Regions
	Nucleotide binding	91 – 102	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q2NEB6 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 6E577DDCB2604F0F	FASTA	723	79,171
	10 20 30 40 50 60 MVLTDDELKY IEEVLGRKPN ELELGMMDVM FSEHCSYKSS KPILRNFPTD GPDVILGPGD 70 80 90 100 110 120 DAGIVSLTDE YALAIGMESH NHPSAVEPYG GAGTGIGGIV RDIISMGAKP VVLLDALRFG 130 140 150 160 170 180 HMSNQRSKYI FDYVVKGISD YGNRIGVPTV GGEIEFDDNF QYNPMVNVVC AGLVKKDEIV 190 200 210 220 230 240 YGSAPIVGDV YLLMGGTTGR DGIHGVTFAS EELTSNSEVE DRPAVQVADP FTKKRVMEAT 250 260 270 280 290 300 YELLETLDIH GVKDLGGGGL TCCLSEMADK GGNGSLIDLN KIPLREENMT PYEIMLSESQ 310 320 330 340 350 360 ERMVFAIDSS DVEKAFEICK KHDLAHAIIG NIIDKREFII KDDEEEICHA PNILFTEAPI 370 380 390 400 410 420 IEREAKEPEK IIEDVEIPEV DVEEALYKLL SSENITSKEW VYSQYDHEVQ LRTIVKPGDD 430 440 450 460 470 480 AAVIKVDDET SFTIGTDCNS THVLLNPYDG AASAVLESIN NAISMGARPI ALVDCLNFGN 490 500 510 520 530 540 PEKPEVFWQF KEAVSGMSDV AGKFNTPFIS GNVSFYNETE GVTVNPSPIV GTVGVVENTK 550 560 570 580 590 600 IKTMTLKDDG DVILVVGNTY PELDGSQYYK HIHNVVQGYP PKIRLEENYN VSTTIEKLIP 610 620 630 640 650 660 KYNDNITSVH DISKGGLIIA LALMSIKSKK GVNVNISNIP STTELSQDDK LYSESNSRFI 670 680 690 700 710 720 ITVKEDALTT IEEALSKNNV AYSVIGSVCS DGFKISNDND VLVDCSVEKI TKENTTTIEK QMA

References

[1]

"The genome sequence of Methanosphaera stadtmanae reveals why this human intestinal archaeon is restricted to methanol and H2 for methane formation and ATP synthesis." Fricke W.F., Seedorf H., Henne A., Kruer M., Liesegang H., Hedderich R., Gottschalk G., Thauer R.K. J. Bacteriol. 188:642-658(2006) [PubMed: 16385054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 3091.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000102 Genomic DNA. Translation: ABC57837.1.
RefSeq	YP_448480.1. NC_007681.1.
3D structure databases
ProteinModelPortal	Q2NEB6.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2NEB6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3856099.
GenomeReviews	Gene locus Msp_1467 in contig CP000102_GR.
KEGG	mst:Msp_1467.
NMPDR	fig|339860.6.peg.1402.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04101.
HOGENOM	HBG311214.
OMA	YGNSFGV.
PhylomeDB	Q2NEB6.
ProtClustDB	PRK01213.
Enzyme and pathway databases
BioCyc	MSTA339860:MSP_1467-MONOMER.
Family and domain databases
HAMAP	MF_00420. PurL_2. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_METST

Accession

Primary (citable) accession number: Q2NEB6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	February 7, 2006
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program