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Q2NEA8 (GLYA_METST) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Pyridoxal-phosphate-dependent serine hydroxymethyltransferase
Short name=SHMT
Short name=Serine methylase
EC=2.1.2.1
Gene names
Name:glyA
Ordered Locus Names:Msp_1475
OrganismMethanosphaera stadtmanae (strain DSM 3091) [Complete proteome] [HAMAP]
Taxonomic identifier339860 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanosphaera

Protein attributes

Sequence length422 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate serving as the one-carbon carrier By similarity.

Catalytic activity

5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. HAMAP MF_00051

Cofactor

Pyridoxal phosphate By similarity. HAMAP MF_00051

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_00051

Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1.

Subcellular location

Cytoplasm By similarity HAMAP MF_00051.

Sequence similarities

Belongs to the SHMT family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 422422Pyridoxal-phosphate-dependent serine hydroxymethyltransferase
PRO_0000235054

Regions

Region120 – 1223Substrate binding By similarity

Sites

Binding site301Pyridoxal phosphate By similarity
Binding site501Pyridoxal phosphate By similarity
Binding site521Substrate By similarity
Binding site601Pyridoxal phosphate By similarity
Binding site1711Pyridoxal phosphate By similarity
Binding site1991Pyridoxal phosphate By similarity
Binding site2251Pyridoxal phosphate By similarity
Binding site3591Pyridoxal phosphate By similarity

Amino acid modifications

Modified residue2261N6-(pyridoxal phosphate)lysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2NEA8 [UniParc].

Last modified February 7, 2006. Version 1.
Checksum: A741596C970F2876

FASTA42246,447
        10         20         30         40         50         60 
MSNLEQAERI KNLTKDHHNW MKNSLNLIAS ENITSRAVRE AVASDLSHRY AEGLPGERLY 

        70         80         90        100        110        120 
EGCDYIDAIE EETIALSKKL YDAEHVNVQP TSGVVANLAS FFALTKPGDL LMSINVPEGG 

       130        140        150        160        170        180 
HISHASVSAA GIRGLKISSV PMDDSIMNVD IDKTLSKIRE KEPKAIVLGG SLFLFPQPVS 

       190        200        210        220        230        240 
EVADVAKEVG AKIIYDAAHV LGLIAGKRFQ DPVKEGADIV TGSTHKTFPG PQGGIILCKE 

       250        260        270        280        290        300 
EIGRKVDNCV FPGVVSNHHL HHMAALGVAT AEMLEFGKDY ANQTISNAKA LAQALYERGF 

       310        320        330        340        350        360 
NVLCEDQGFT ESHQVAMDVA KLGDVSKMAK TLQYNNIILN KNLLPWDDVN DSDNPSGIRM 

       370        380        390        400        410        420 
GTQELTHRGF KEDEMDQVAE FIKQVVMDKK DVKEDVTEFM QDYTTVHYAF DEGCEGYDYI 


EF

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References

[1]"The genome sequence of Methanosphaera stadtmanae reveals why this human intestinal archaeon is restricted to methanol and H2 for methane formation and ATP synthesis."
Fricke W.F., Seedorf H., Henne A., Kruer M., Liesegang H., Hedderich R., Gottschalk G., Thauer R.K.
J. Bacteriol. 188:642-658(2006) [PubMed: 16385054] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 3091.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000102 Genomic DNA. Translation: ABC57845.1.
RefSeqYP_448488.1. NC_007681.1.

3D structure databases

ProteinModelPortalQ2NEA8.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ2NEA8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3856107.
GenomeReviewsGene locus Msp_1475 in contig CP000102_GR.
KEGGmst:Msp_1475.
NMPDRfig|339860.6.peg.1409.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGarNOG04649.
HOGENOMHBG301263.
OMAMILTNHE.
ProtClustDBPRK00011.

Enzyme and pathway databases

BioCycMSTA339860:MSP_1475-MONOMER.

Family and domain databases

HAMAPMF_00051. SHMT.
[Tree]
InterProIPR015424. PyrdxlP-dep_Trfase_major_dom.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR001085. Ser_HO-MeTrfase.
IPR019798. Ser_HO-MeTrfase_PLP_BS.
[Graphical view]
Gene3DG3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
KOK00600.
PANTHERPTHR11680. Gly_HO-Metrfase. 1 hit.
PfamPF00464. SHMT. 1 hit.
[Graphical view]
PIRSFPIRSF000412. SHMT. 1 hit.
SUPFAMSSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITEPS00096. SHMT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLYA_METST
AccessionPrimary (citable) accession number: Q2NEA8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: February 7, 2006
Last modified: January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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