Q2NE12 (SYR_METST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arginine--tRNA ligase
EC=6.1.1.19

Alternative name(s):
Arginyl-tRNA synthetase
Short name=ArgRS

Gene names

Name:	argS
Ordered Locus Names:	Msp_1574

Organism

Methanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3) [Complete proteome] [HAMAP]

Taxonomic identifier

339860 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanosphaera › Methanosphaera stadtmanae

Protein attributes

Sequence length	560 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg). HAMAP-Rule MF_00123
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00123.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	arginyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP arginine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 560	560	Arginine--tRNA ligase HAMAP-Rule MF_00123		PRO_0000242131
Regions
	Motif	122 – 132	11	"HIGH" region HAMAP-Rule MF_00123

Sequences

	Sequence		Length	Mass (Da)
	Q2NE12 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 23080817D7E97A69	FASTA	560	64,597
	10 20 30 40 50 60 MYSKLKTEIN ESIKEALTKL RIKYDDEIIL EEPPNPSMGD MSTNIAFSLA SKLKKSPVEI 70 80 90 100 110 120 AQEIKENIKL PLYFEKVETK GPYINFYINY TLFTTKVVNY IDKNYGELPE KDERILLEHT 130 140 150 160 170 180 SANPNGPLHV GHLRNAILGD SLKRILQHAG YKVEAQYYVN DMGRQIAIIV WGMDKFNYTV 190 200 210 220 230 240 DDDKKADHAI GEVYYKCNQQ LEANPEYNQE IDDILRKYEE GTDAALIDAF QGVVEYCIDG 250 260 270 280 290 300 IKETLKDLNI KMNLFKWEST FLRNGSVDDV LEKLQPFTIQ KDILYLPLER YNVDKELVLR 310 320 330 340 350 360 RSNGTSLYAT RDLAYHQYKT KNSDISLDIL GADHKLAAKQ LGLALELSNN RAPEVVFYEF 370 380 390 400 410 420 IDLPEGSMST RKGVFISVDE FIEQSVEHAK EELIRRDLDL TEKQIEEVSK IVGVGSIRFY 430 440 450 460 470 480 INQISPEKPI TFKWEEALSF ERGCASIQYA HARACKLLAK SDYNEFEEVR CDYELDDEEK 490 500 510 520 530 540 DLIKTLSQFT EVICQSAQER RVHHLAQYTL SLSKAFNKFY KSKQVIGSEH EKLRLKLVDA 550 560 SRITLKNSLK LLGIKSPEFM

References

[1]

"The genome sequence of Methanosphaera stadtmanae reveals why this human intestinal archaeon is restricted to methanol and H2 for methane formation and ATP synthesis." Fricke W.F., Seedorf H., Henne A., Kruer M., Liesegang H., Hedderich R., Gottschalk G., Thauer R.K. J. Bacteriol. 188:642-658(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000102 Genomic DNA. Translation: ABC57941.1.
RefSeq	YP_448584.1. NC_007681.1.
3D structure databases
ProteinModelPortal	Q2NE12.
ModBase	Search...
Protein-protein interaction databases
STRING	339860.Msp_1574.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABC57941; ABC57941; Msp_1574.
GeneID	3855800.
KEGG	mst:Msp_1574.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0018.
HOGENOM	HOG000247213.
KO	K01887.
OMA	HTSANPN.
ProtClustDB	PRK01611.
Enzyme and pathway databases
BioCyc	MSTA339860:GJEZ-1577-MONOMER.
Family and domain databases
Gene3D	3.30.1360.70. 1 hit. 3.40.50.620. 1 hit.
HAMAP	MF_00123. Arg_tRNA_synth.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR001278. Arg-tRNA-ligase_Ia. IPR015945. Arg-tRNA-synth_Ia_core. IPR005148. Arg-tRNA-synth_N. IPR008909. DALR_anticod-bd. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
PANTHER	PTHR11956. PTHR11956. 1 hit.
Pfam	PF03485. Arg_tRNA_synt_N. 1 hit. PF05746. DALR_1. 1 hit. PF00750. tRNA-synt_1d. 1 hit. [Graphical view]
PRINTS	PR01038. TRNASYNTHARG.
SMART	SM01016. Arg_tRNA_synt_N. 1 hit. SM00836. DALR_1. 1 hit. [Graphical view]
SUPFAM	SSF55190. Arg-tRNA-synth_Ic_N. 1 hit. SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00456. argS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYR_METST

Accession

Primary (citable) accession number: Q2NE12

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	February 7, 2006
Last modified:	May 29, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program